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Pituitary adenylate cyclase-activating polypeptide (PACAP-38), a 38 amino acid peptide, was recently isolated from the ovine hypo-thalamus11). Immunoreactivity of PACAP has been identified in the hypothalamus, including both the external and internal zones of the median eminence10). Specific PACAP-binding sites are present on rat anterior pituitary cell membranes5) and in the hypothalamus of cows12). The measurement of intracellular free calcium has shown that the somatotrophs in the rat pituitary are the direct targets of PACAP2). Growth hormone (GH)-releasing Institute of Bioscience and Human Technology, Japan for providing hpGRF (1-29)-NH2, and

The effect of angiotensin II (A II) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF). A II significantly stimulated GH release added in combined treatment with the GHRH and SRIF, the inhibitory effect of the SRIF was significantly less than that of SRIF with the GHRH alone or with the A II alone (P<0.05). These results suggest that A II could have interacted with GHRH and SRIF to modulate GH secretion in vitro in cattle.

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