Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors

Gabrielson, Edward; Gerwin, Brenda I.; Harris, Curtis C.; Roberts, Anita B.; Sporn, Michael B.; Lechner, John F.

doi:10.1096/fasebj.2.11.3260881

Cited by 81 publications

(39 citation statements)

References 14 publications

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“…Although TGF-/?, in general, inhibits the proliferation of epithelial cells [7], TGF-/3 stimulates some epithelial cells, such as normal human mesothelial cells [8]. Moreover, TGF-/3 augments hyaluronic acid production by normal human mesothelial cells and fibroblasts [10,13].…”

Section: Discussionmentioning

confidence: 99%

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Transforming growth factor-beta 1 (TGF-β1)- and β2-like activities in malignant pleural effusions caused by malignant mesothelioma or primary lung cancer

Maeda

Ueki

Ohkawa

et al. 1994

Clinical and Experimental Immunology

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SUMMARYWe investigated the levels of TGF-/? in malignant pleural effusions (MPE) caused by malignant mesothelioma (MESO) or primary lung cancer. TGF-/? levels in MPE caused by MESO were 283-9 ± 219-2pM (mean is.d.) and were three to six times higher than those due to primary lung cancers (P < 0-01 or P < 0-05). We also evaluated TGF-/?1-and /32-like activities in MPE using specific polyclonal antibodies. Although TGF-/?l-like activity could be detected in all cases, TGF-^2-like activities were detected in five of seven in MESO and in a few cases with primary lung cancer. These results demonstrate that the levels of total TGF-/? and TGF-/?2-like activity may be clinically useful to differentiate MESO from primary lung cancer. Our data also suggest that TGF-/? may help further characterize the clinical features of MESO.Keywords TGF-jSl TGF-,32 malignant pleural effusion malignant mesothelioma primary lung cancer

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Section: Discussionmentioning

confidence: 99%

“…TGF-/? is unique in its ability to stimulate the proliferation of nonnal humati mesothelial cells, epithelial cells which originate from mesoderm [8]. Moreover, TGF-/?…”

Section: Introductionmentioning

confidence: 99%

Transforming growth factor-beta 1 (TGF-β1)- and β2-like activities in malignant pleural effusions caused by malignant mesothelioma or primary lung cancer

Maeda

Ueki

Ohkawa

et al. 1994

Clinical and Experimental Immunology

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“…In attempts to identify the factor responsible, we used blocking antibodies to well-known cytokines. MC are known to have receptors for PDGF, TNF-a, TGF-a and -,B (13)(14)(15), and when antibodies to these molecules were added to the rat MC in the presence of PLLF, no inhibition of the growth increase was observed. This is further evidence that the cytokine effect on MC cells is different from that which stimulates fibroblast growth.…”

Section: Discussionmentioning

confidence: 99%

“…In this study we examine differential responses of lung MC after long-or shortfiber deposition in the lung, to determine if This paper is based on a presentation at The Sixth International Meeting on the Toxicology of Natural and ManMade Fibrous and Non-Fibrous Particles held [15][16][17][18] September 1996 in Lake Placid, New York. Manuscript received at EHP27 March 1997; accepted 6 …”

Section: Introductionmentioning

confidence: 99%

Early mesothelial cell proliferation after asbestos exposure: in vivo and in vitro studies.

Adamson

1997

Environ Health Perspect

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There is some evidence that proliferation of pleural mesothelial cells (MC) occurs soon after deposition of asbestos fibers. To study this effect, we instilled a single dose of 0.1 mg crocidolite into the lungs of mice for 1 and 6 weeks and counted labeled nuclei after 3H-thymidine (3HTI) injection. Short fibers (<1 pm) induced little change in the lung; they were mostly phagocytized and had a minimal effect on MC labeling. Long fibers up to 20 pm damaged the bronchiolar epithelium and were incorporated into connective tissue. Increased 3HT uptake was seen in fibroblasts and epithelial cells and also in MC, which peaked at 2% labeled at 1 week compared to near 0% labeling in controls. No fibers were found in or near labeled MC, which suggested that a cytokine generated in the lung during the early response phase might induce MC proliferation. To look for a cytokine effect in vitro, we instilled asbestos into rat lungs and, after 1 and 6 weeks, bronchoalveolar and pleural lavage fluids as well as macrophages were collected. Alveolar macrophages contained fibers, but pleural macrophages (PM) did not. After short-term culture, macrophage supernatants and the lavage fluids were tested on rat lung MC in culture. At 1 week, PM secreted growth factor(s) for MC, and the mitogenic effect was more pronounced with lavage fluids. No effects on MC were found using material prepared 6 weeks after asbestos. The early MC growth increase was not blocked by antibodies to cytokines, such as plateletderived growth factor, fibroblast growth factors, or tumor necrosis factor, but was inhibited by anti-keratinocyte growth factor (anti-KGF). The results show that an early growth phase of MC after asbestos exposure appears unrelated to particle translocation to the pleura but is associated with cytokine release, most likely KGF, by lung cells.

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“…Mesothelial cells are capable of responding to a wide range of growth factors in monolayer (Gabrielson et al, 1988;Laveck et al, 1988) and it is quite likely that the growth factor content of the fluids is pleomorphic. Whilst TGF-.a may be implicated, other results exclude not only TGF-o, but also indicate that the growth factor is specific to ovarian cancer (Mills et al, 1988).…”

Section: Tgf-pmentioning

confidence: 99%

A comparison of the growth promoting properties of ascitic fluids, cyst fluids and peritoneal fluids from patients with ovarian tumours

Wilson

Fox²,

Scott³

et al. 1991

Br J Cancer

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Summary The growth promoting properties of ascitic fluids, cyst fluids and peritoneal fluids from patients with ovarian malignancy, benign ovarian tumours and non-tumour related gynaecological conditions have been investigated using an ovarian carcinoma cell line (OAW 42) (La Rocca & Rheinwald, 1985). Materials and methods Patient groupsAscitic fluids were collected from ovarian cancer patients (n = 42), non-ovarian cancer patients (n = 13), non-tumour patients (n = 4) and one patient with a benign ovarian tumour. Peritoneal fluids were collected from gynaecology patients undergoing laporotomy or laporoscopy for nontumour related conditions (n = 15), second-look laporotomies in ovarian cancer patients (n = 5), patients with benign ovarian tumours (n = 8) and with malignant ovarian tumours (n = 10). Cyst fluids were collected from borderline tumours (n = 3), benign tumours (n = 23) and malignant tumours (n = 11). All fluids were collected without heparin, centrifuged at 3,000 r.p.m. for 15 min to remove cells, aliquotted and frozen at -20°C.

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Stimulation of DNA synthesis in cultured primary human mesothelial cells by specific growth factors

Cited by 81 publications

References 14 publications

Transforming growth factor-beta 1 (TGF-β1)- and β2-like activities in malignant pleural effusions caused by malignant mesothelioma or primary lung cancer

Transforming growth factor-beta 1 (TGF-β1)- and β2-like activities in malignant pleural effusions caused by malignant mesothelioma or primary lung cancer

Early mesothelial cell proliferation after asbestos exposure: in vivo and in vitro studies.

A comparison of the growth promoting properties of ascitic fluids, cyst fluids and peritoneal fluids from patients with ovarian tumours

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