Transforming growth factor-beta 1 (TGF-β1)- and β2-like activities in malignant pleural effusions caused by malignant mesothelioma or primary lung cancer

Abstract

SUMMARYWe investigated the levels of TGF-/? in malignant pleural effusions (MPE) caused by malignant mesothelioma (MESO) or primary lung cancer. TGF-/? levels in MPE caused by MESO were 283-9 ± 219-2pM (mean is.d.) and were three to six times higher than those due to primary lung cancers (P < 0-01 or P < 0-05). We also evaluated TGF-/?1-and /32-like activities in MPE using specific polyclonal antibodies. Although TGF-/?l-like activity could be detected in all cases, TGF-^2-like activities were detected in five of seven in MESO and in a few cases with primary lung cancer. These results demonstrate that the levels of total TGF-/? and TGF-/?2-like activity may be clinically useful to differentiate MESO from primary lung cancer. Our data also suggest that TGF-/? may help further characterize the clinical features of MESO.Keywords TGF-jSl TGF-,32 malignant pleural effusion malignant mesothelioma primary lung cancer

Latent TGF-β binding proteins (LTBPs) 1 and 3 differentially regulate transforming growth factor-β activity in malignant mesothelioma

“…2A). In agreement with previous studies [22,23], TGF-β1 was up-regulated in all 4 and TGF-β2 in 3 of 4 MM samples. TGF-βs are secreted as large latent complexes containing one of the TGF-β binding LTBPs, which participate in the activation process.…”

“…P-Smad2 immunoreactivity was high especially in the tumor tissue, which is in accordance with observations on increased TGF-β levels in MM [22,23]. To elucidate LTBP-mediated TGF-β activation, nontumorigenic Met5A cells and 2 mesothelioma cell lines (M14K and M38K) were selected for in vitro studies.…”

A Novel Small-Molecule Inhibitor of Transforming Growth Factor β Type I Receptor Kinase (SM16) Inhibits Murine Mesothelioma Tumor Growth In vivo and Prevents Tumor Recurrence after Surgical Resection

“…15). Pleural fluids from mesothelioma patients contain TGFh levels that are 3-to 6-fold elevated compared with primary lung cancers (19,20). Several mesothelioma cell lines also overexpress TGFh (reviewed in ref.…”

ERK1/2 and p38 MAP kinase control MMP‐2, MT1‐MMP, and TIMP action and affect cell migration: A comparison between mesothelioma and mesothelial cells

“…In the subsequently described study we focused on two mesothelioma associated growth factors, transforming growth factor (TGF-b 1 ) and platelet derived growth factor (PDGF)-BB which both are found in bronchoalveolar lavage fluids obtained from pleural malignant mesothelioma patients and in cell culture systems of pleural malignant mesothelioma (Gerwin et al, 1987;Overall et al, 1991;Garlepp et al, 1993;Maeda et al, 1994;Fitzpatrick et al, 1995;Langerak et al, 1996). TGF-b 1 and PDGF-BB has been shown to modulate the expression of gelatinolytic MMPs and TIMPs in several lung cell types, and both factors also regulate cell proliferation and cell migration, via MMPs (Manning et al, 1991;Overall et al, 1991;Papakonstantinou et al, 1998;Eickelberg et al, 1999).…”