Statistical optimization of airlift photobioreactor for high concentration production of torularhodin pigment

Varmira, Kambiz; Habibi, Alireza; Moradi, Samaneh; Bahramian, Elham

doi:10.1016/j.bcab.2016.09.013

Cited by 15 publications

(15 citation statements)

References 15 publications

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“…In fact, the cultivation of R. pallida on glycerol containing medium resulted in the highest total carotenoid concentration (87 µg g -1 of cell) on the third day. The decrease of concentration of total carotenoids over a more extended time was already reported by Varmira et al (2016) and Varzakakou et al (2011) for the growth of fungi in bioreactors. According to these authors the decrease in pigment concentration with time is due to cell membrane autolysis and chemical oxidation reactions of pigments.…”

Section: Selection Of Rhodotorula Strain For Carotenoids Productionsupporting

confidence: 74%

“…β-Carotene is considered the most interesting carotenoid type, because it is the main safe dietary source of vitamin A and its antioxidant activity (Kot et al, 2016). As reported in the literature, the oxidation of carotenes to torulene and torularhodin is promoted by microbial cultures under oxidative conditions (Varmira et al, 2016;Zoz et al, 2015). Currently, torulene and torularhodin are not commercially used.…”

Section: Assessing Low-cost Materials As Carbon and Nitrogen Sources mentioning

confidence: 99%

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Valorization of Solid Wastes From the Brewery and Biodiesel Industries for the Bioproduction of Natural Dyes

Rodrigues

Schueler

Silva

et al. 2019

Braz. J. Chem. Eng.

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This study aims to assess a new approach for concomitant valorization of two industrial wastes -raw glycerin and spent brewer's yeast -for the bioproduction of valuable carotenoids. Microbial pigments have numerous applications in the food and cosmetic industries. First, four cultures of yeasts were screened using pure glycerol and either (NH 4 ) 2 SO 4 or urea as carbon and nitrogen sources, respectively. The ability of the best performing culture to accumulate carotenoids was investigated in a medium in which only wastes were supplemented as carbon and nitrogen sources. All the fermentations were carried out in 500 mL-Erlenmeyer flasks containing 150 mL of the medium. Microbial culture was incubated at 30 °C and 150 rpm for 120 h. Particularly, R. marina was the strain with the biggest potential to produce total carotenoids (up to 420 μg g -1 ). The four major carotenoid pigments identified by LCAPCI-MS were β-carotene, γ-carotene, torulene, and torularhodin. All fermentation assays and all the determinations were performed in triplicate. Results show that the bioprocess proposed in this work is technically and environmentally feasible, and sustainable. The simultaneous use of raw glycerin and spent brewer's yeast for the production of carotenoids by R. marina is reported for the first time.

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Section: Selection Of Rhodotorula Strain For Carotenoids Productionsupporting

confidence: 74%

Section: Assessing Low-cost Materials As Carbon and Nitrogen Sources mentioning

confidence: 99%

Valorization of Solid Wastes From the Brewery and Biodiesel Industries for the Bioproduction of Natural Dyes

Rodrigues

Schueler

Silva

et al. 2019

Braz. J. Chem. Eng.

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“…The light irradiation intensity was adjusted at 3000 Lux using a control system . On the basis of the previous works, the cultivation temperature was set at 30 ± 2 °C using a thermostat chamber and the initial pH of the medium was adjusted at 6.5 using 2 N HCl solution . In order to prevent of foam formation during fermentation in the reactor, soya oil (1%v) as an antifoam agent was added to the cultivation medium.…”

Section: Methodsmentioning

confidence: 99%

“…Most of the previous research on the fermentation process has been concerned with practical aspects of improving the overall carotenoid yield from producing cells by studying the effect of some variables as temperature, pH, nutrient concentration, mineral type, agitation speed, aeration, and light irradiation intensity . From an engineering point of view, the rate of the reaction in industrial processes is a principal factor affecting the cost effectiveness.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of continuous production of β‐carotene in an airlift bioreactor

Taavoni

Habibi

Varmira

et al. 2017

Asia-Pacific J Chem Eng

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The focus in the present work was on the continuous β-carotene production from free fatty acids supplied by the soap stock as a sole carbon source for Rhodotorula rubra cells in an airlift bioreactor. The effects of dilution rate on the cell growth and β-carotene production were studied by both freely suspended-and immobilized-cell systems in the chemostat operations. The results showed that the immobilization of the cell on natural sponge of loofah significantly increased the optimum and maximum dilution rates from 0.0375 to 0.05 hr −1 and 0.055 to 0.150 hr −1 , respectively. The immobilization process has practical role in balancing of cell proliferation in the matrix and wash out the grown cells containing β-carotene from the bioreactor where β-carotene productivity was enhanced from 5.4 to 23.4 mg L −1 hr −1 using the immobilized cell technology.

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“…KF-104 YG medium 1.74 1.92 [ 42 ] Rhodotorula glutinis mutant 32 Glucose 5 3 [ 43 ] Rhodotorula glutinis var. glutinis Wort 0.53 0.16 [ 44 ] Rhodotorula rubra ICCF 209 Glucose + 0.1% oleic acid – 0.31 [ 45 ] MS3 medium – 0.71 Fructose – 0.40 Rhodotorula rubra GED8 Glucose 0.87 1.22 [ 46 ] Rhodotorula rubra PTCC 5255 Shaken culture 5.09 7.80 [ 47 ] Cultivation in a biofermentor, irradiation at 1780 lx 4.48 35.59 Rhodotorula mucilaginosa RCL-11 Control 0.82 mg/L 2.24 mg/L [ 48 ] + CuSO 4 2.33 mg/L 5.37 mg/L + H 2 O 2 8.21 mg/L 5.22 mg/L + CuSO 4 + H 2 O 2 5.60 mg/...…”

Section: Structure and Sources Of Torulene And Torularhodinmentioning

confidence: 99%

Torulene and torularhodin: “new” fungal carotenoids for industry?

et al. 2018

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Torulene and torularhodin represent the group of carotenoids and are synthesized by yeasts and fungi. The most important producers of these two compounds include yeasts of Rhodotorula and Sporobolomyces genera. The first reports confirming the presence of torulene and torularhodin in the cells of microorganisms date to the 1930s and 1940s; however, only in the past few years, the number of works describing the properties of these compounds increased. These compounds have strong anti-oxidative and anti-microbial properties, and thus may be successfully used as food, feedstock, and cosmetics additives. In addition, tests performed on rats and mice showed that both torulene and torularhodin have anti-cancerous properties. In order to commercialize the production of these two carotenoids, it is necessary to obtain highly efficient yeast strains, for example, via mutagenization and optimization of cultivation conditions. Further studies on the activity of torulene and torularhodin on the human body are also needed.

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Statistical optimization of airlift photobioreactor for high concentration production of torularhodin pigment

Cited by 15 publications

References 15 publications

Valorization of Solid Wastes From the Brewery and Biodiesel Industries for the Bioproduction of Natural Dyes

Valorization of Solid Wastes From the Brewery and Biodiesel Industries for the Bioproduction of Natural Dyes

Kinetics of continuous production of β‐carotene in an airlift bioreactor

Torulene and torularhodin: “new” fungal carotenoids for industry?

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