Kinetics of continuous production of <scp>β</scp>‐carotene in an airlift bioreactor

Taavoni, Shoeib; Habibi, Alireza; Varmira, Kambiz; Alipour, Sajad

doi:10.1002/apj.2160

Cited by 9 publications

(3 citation statements)

References 40 publications

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“…2 where the specific carotenoids production rate has relatively similar trend to the specific cell growth rate and reaches to a highest value about 2.233 μg.gcell -1 h -1 at initial CSL concentration of 10 vv -1 %. This observation agreed with previous works showed a linear relationship between the RP and μ and it indicating carotenoids are associated growth products [22]. Further increase of initial CSL concentration from 10 vv -1 % to 40 vv -1 % resulted in decrease of RP from 2.233 μg gcell -1 h -1 to 0.524 μg gcell -1 h -1 due to the inhibitory effects of CSL.…”

Section: Shaken Flask Experimentssupporting

confidence: 92%

Batch and Fed-Batch Production of β-carotene by Rhodotorula Toruloides KP324973 Using Corn Steep Liquor As Sole Carbon Source

Habibi

Fallahi

Abbasi

et al. 2021

Preprint

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Application of agro-industrial waste in microbial fermentation is interesting in economic and environmental aspects. Carotenoids production by Rhodotorula toruloides KP324973 was investigated using corn steep liquor (CSL) as sole carbon source. Haldane model with constants µmax = 0.056 h-1, KS = 1.54 vv-1%, and KI = 58.58 vv-1% showed best describe of cell growth kinetics on CLS. A same maximum carotenoid production rate (Rp) about 2.23 μg gcell-1 h-1 was obtained at initial CSL concentration of 5 v/v% after 72 h and 21 h in batch cultivation in shaken flasks and bubble column reactor (BCR), respectively. Further improvement of carotenogenesis was followed by fed-batch cultivation in BCR where the optimal setting of factors at feed flow rate of 5 mL h-1, pH of 5.66, and temperature 14 ˚C gained a highest Rp = 8.686 μg gcell-1 h-1. Chromatographic analysis showed more than 94% of produced carotenes was β-carotene.

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Section: Shaken Flask Experimentssupporting

confidence: 92%

Batch and Fed-Batch Production of β-carotene by Rhodotorula Toruloides KP324973 Using Corn Steep Liquor As Sole Carbon Source

Habibi

Fallahi

Abbasi

et al. 2021

Preprint

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“…As recently reviewed by [40], yeast biofilm formation in food and food processing plants could be considered as a positive or negative phenotype. Here, the high biofilm forming ability coupled with the high carotenoid content of the strains in cluster 5 suggests their possible utilization for the continuous production of carotenoids in bioreactors where yeast cells are immobilized on inert surfaces [61]. In addition, the high biomass obtained by growing these strains on glycerol suggests the use of these strains for the valorization of such by-products, thus increasing the sustainability of carotenoid production [26].…”

Section: Discussionmentioning

confidence: 93%

Exploring the Biodiversity of Red Yeasts for In Vitro and In Vivo Phenotypes Relevant to Agri-Food-Related Processes

et al. 2020

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Red yeasts grow on food wastes, show antagonistic activity against food-spoilage microorganisms, produce food supplements, and may be utilized as feed-supplements themselves to positively modulate the quali-quantitative composition of intestinal microbiota. Therefore, they show a variety of possible biotechnological applications in agri-food-related processes. Here, to further explore the biotechnological potential of red yeasts, eleven strains ascribed to different species of the genera Rhodotorula and Sporobolomyces, differing for biomass and carotenoids production, were characterized in vitro for biofilm formation, invasive growth, and growth at the temperature range of 20–40 °C and in vivo for their antagonistic activity against the fungal pathogen and patulin producer Penicillium expansum. Most of them formed cellular MAT and showed invasive growth as well as adhesion to plastic materials. Four strains determined a significant reduction of fruit decay caused by P. expansum on apple fruit while the remaining seven showed different degrees of biocontrol activity. Finally, none of them grew at body temperature (>37 °C). Statistical analyses of both qualitative and quantitative phenotypic data, including biomass and carotenoids production, gathered further information on the most interesting strains for the biotechnological exploitation of red yeasts in agri-food-related process.

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“…When the airlift bioreactor was considered for Yarrowia lipolytica growth, the material balance could be calculated as follows (Equations ( 3)-( 5)) [24]:…”

Section: Kineticsmentioning

confidence: 99%

Screening the Lipid Production Potential of Oleaginous Yeast Yarrowia lipolytica under Wood Hydrolysates

2023

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Since Yarrowia lipolytica, an oily yeast, contains many valuable products as biomass, to increase its widespread use, it is very important to develop a sustainable production model. In this study, the production of high acid hydrolysate from sawdust biomass, which is produced in very high amounts in our environment, and the effect of these products on the growth efficiency of Yarrowia lipolytica were investigated. A preliminary statistical analysis was carried out to assess sugar production from sawdust by hydrolysis, which estimated 85% of total sugar recovery (TSR) using 96 g of sawdust, while TSR at the highest sawdust was as high as 79%. There was a higher loss in the recovery of C5 sugars with acid and time. This study revealed that Y. lipolytica could grow with high biomass yields in varying hydrolysate concentrations, approaching yields achieved in synthetic glucose media. The maximum biomass obtained was 13 and 26 g in batch and airlift operations, respectively, using organic nutrients. Biomass and lipid yields from kinetic modeling provided a close approximation to the experimental yields performed on an airlift reactor. It was found that Y. lipolytica biomass cultivated on sawdust hydrolysates had significant lipid and protein compositions, as high as 29 and 38%, respectively.

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Kinetics of continuous production of β‐carotene in an airlift bioreactor

Cited by 9 publications

References 40 publications

Batch and Fed-Batch Production of β-carotene by Rhodotorula Toruloides KP324973 Using Corn Steep Liquor As Sole Carbon Source

Batch and Fed-Batch Production of β-carotene by Rhodotorula Toruloides KP324973 Using Corn Steep Liquor As Sole Carbon Source

Exploring the Biodiversity of Red Yeasts for In Vitro and In Vivo Phenotypes Relevant to Agri-Food-Related Processes

Screening the Lipid Production Potential of Oleaginous Yeast Yarrowia lipolytica under Wood Hydrolysates

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