Staphylococcus Aureus in Nursing Professionals and the Microorganism’s Susceptibility Profile to Antimicrobials

Lopes, Letícia Pimenta; Pio, Daiana Patrícia Marchetti; Reinato, Lilian Andreia Fleck; Gaspar, Gilberto Gambero; Prado, Marinésia Aparecida do; Gir, Elucir

doi:10.1590/0104-07072017000400016

Cited by 4 publications

(9 citation statements)

References 19 publications

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“…A transmissão de microrganismos pode acontecer por meio do contato direto ou indireto com pacientes e suas imediações, podendo variar de acordo com a atividade realizada, uma vez que a pele é um potencial reservatório de microrganismos e possui a capacidade de transferi-los de uma superfície à outra. Cabe destacar que as técnicas de higiene das mãos padronizadas e recomendadas podem reduzir consideravelmente a carga microbiana das mãos dos profissionais de saúde e, ainda, especialmente da equipe de enfermagem, a qual é identificada como mais suscetível à infecção por múltiplos microrganismos resistentes em seu ambiente de trabalho, pelas características de suas atividades (2,8) .…”

Section: Discussionunclassified

“…As infecções relacionadas com a assistência à saúde são consideradas uma das maiores causas de mortes em todas as idades, além de gerar altos custos aos pacientes e suas famílias e sobrecarregar os sistemas de saúde. Entretanto, são evitáveis por meio de práticas simples e de baixo custo, como a higiene das mãos que se constitui a principal medida para reduzir essas infecções e a disseminação de patógenos com resistência antimicrobiana (8)(9) . Nesta pesquisa, observou-se que, mesmo após a higiene das mãos houve o crescimento bacteriano.…”

Section: Discussionunclassified

“…O crescimento bacteriano de MRSA nas mãos dos profissionais de enfermagem pode ser atribuído ao fato de que em sua atuação há o contato direto com pacientes e superfícies contaminadas, uma vez que o MRSA é transmitido por meio do contato, sendo descrito como o principal agente causador das infecções relacionadas com a assistência à saúde (8,17) .…”

Section: Discussionunclassified

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Bacterial growth on the hands of health care workers: implications for preventing nosocomial infections

Andrade¹,

Brun²,

Brandão³

et al. 2021

Rev Rene

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Objective: to analyze bacterial growth in samples collected from the hands of health professionals after hygiene with soap and water. Methods: cross-sectional analytical study, carried out with healthcare professionals by collecting samples (print) from the digital pulp of the dominant hand on plates containing chromogenic culture medium for microbiological analysis regarding the presence of colonies. Fisher’s and Chi-square tests were used. Results: 73 samples were collected and 67 (91.8%) showed bacterial growth. Methicillin-resistant Staphylococcus aureus was detected in 19 (26.0%). A significant association between colonies with multidrug-resistance profile and the time of performance (p=0.030) and profession (p=0.041) was highlighted. Conclusion: there was bacterial growth in samples after hand hygiene, with higher growth of multidrug-resistant bacteria among nursing professionals and those with longer time of work. These results may contribute to detect the gaps about the measures adopted for infection prevention.

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Bacterial growth on the hands of health care workers: implications for preventing nosocomial infections

Andrade¹,

Brun²,

Brandão³

et al. 2021

Rev Rene

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“…When there is a lack of adherence to standard precautions, healthcare workers, especially nursing professionals (who were associated in the majority of the cases) are certainly more susceptible to colonization by multi-resistant bacteria, and as a result may cause outbreaks [ 6 , 8 ]. However, the most common form of transmission, which favors the dissemination of these bacteria among patients and healthcare workers, is through contact that involves the hands [ 6 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon

et al. 2021

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Background Healthcare workers are susceptible to colonization by multiresistant bacteria, which can increase the risk of outbreaks. Methods Samples were collected from the nasopharynx, hands, and lab coats of healthcare workers. The phenotypic identification was carried out using a VITEK®2 rapid test system. PCR tests for the mecA gene and the sequencing of the amplicons were performed. Staphylococcus epidermidis and Staphylococcus aureus phylogenies were reconstructed using the Bayesian inference. Results A total of 225 healthcare workers participated in this study. Of these, 21.3% were male and 78.7% female. S. epidermidis and S.aureus showed high levels of resistance to penicillin, ampicillin, erythromycin, tetracycline and cefoxitin. The prevalence of methicillin resistant S. aureus was 3.16% and methicillin resistant S. epidermidis was 100%. Multilocus sequence typing identified 23 new S. epidermidis sequence types, and one new allele and sequence type for S. aureus. The frequency of methicillin-resistant S. epidermidis in nursing and hemotherapy technicians as a percentage of the total number of healthcare workers was 5.8–3.1%, while the frequency of methicillin resistant S. aureus in hemotherapy technicians and biomedics, as a percentage of the total number of healthcare workers was 4.2–8.9%%. Conclusions The healthcare workers at the city’s blood bank, even when taking the necessary care with their hands, body and clothes, harbour methicillin-resistant S. aureus and S. epidermidis sequence types, which, as a potential source of multidrug resistant bacteria, can contribute to nosocomial infections among hematological patients.

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“…9 Nursing staff, who have physical proximity to these patients are more susceptible to colonization by this microorganism. 10 Within the hospital environment, healthcare workers may contribute to the nosocomial transmission of S. aureus as reservoirs or vectors. 11 Once colonization occurs, nurses become potential spreaders of microorganisms in health services and may cause outbreaks of infection and compromise patient health.…”

Section: Introductionmentioning

confidence: 99%

Description and Interrelationship Analysis of the Phenotypic and Genotypic Characteristics of MSSA and MRSA Strains Isolated From Healthcare Workers in North-eastern Brazil

Oliveira¹,

Andrade²,

Souza³

et al. 2021

Preprint

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Purpose: This study therefore sought to analyse the interrelationship between the phenotypic and genotypic characteristics of methicillin-susceptible Staphylococcus aureus (MSSA) and MRSA strains isolated from healthcare workers in north-eastern Brazil.Patients and methods: Staphylococcus aureus strains were isolated from nasal mucosa of nursing professionals. They are identified by biochemical analysis and the sensibility drug test were carried out by agar diffusion method. Biofilm formation was detected on the polystyrene plastic surface. Molecular characterization was conducted by polymerase chain reaction (PCR). The Chi-squared or Fisher's exact tests were applied for the analysis of associations between phenotypic and genotypic characteristics.Results: A total of 118 workers were involved, 49.15% of whom presented S. aureus. The most frequently observed strain was resistance to penicillin (86.21%), while the inducible clindamycin resistance (iMLSB) phenotype was detected in 48.84% of isolates. MRSA was found in 41.37% of isolates, while SCCmec type I was seen in 75%. A strain with intermediate resistance to vancomycin was also found. In terms of virulence genes, eta, tst, pvl, spa, clfA, icaA, icaC and icaD genes were detected at least once. Biofilm formation was detected in 89.65% of the strains - 18.97% of these were classified as strongly adherent. No associations with ica genes were detected. Multivariate analyses of clusters demonstrated variability between the strains, with the notable formation of three main clusters, according to phenotypic and genotypic characteristics.Conclusion: High prevalence, multidrug resistance and presence of MRSA strains were all observed. Most isolates had significant virulence genes and the ability to form biofilms, increasing the severity of potential infections. It is therefore necessary to monitor the strains circulating in this hospital and to intensify precautionary measures in order to prevent their spread.

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Staphylococcus Aureus in Nursing Professionals and the Microorganism’s Susceptibility Profile to Antimicrobials

Cited by 4 publications

References 19 publications

Bacterial growth on the hands of health care workers: implications for preventing nosocomial infections

Bacterial growth on the hands of health care workers: implications for preventing nosocomial infections

Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon

Description and Interrelationship Analysis of the Phenotypic and Genotypic Characteristics of MSSA and MRSA Strains Isolated From Healthcare Workers in North-eastern Brazil

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