Standardization of 2-phenoxyethanol as anesthetic for juvenile Brycon cephalus (Gunther, 1869): the use in field procedures

Inoue, L. A. K. A.; Neto, Cristiano dos Santos; Moraes, Gilberto

doi:10.1590/s0103-84782004000200035

Cited by 9 publications

(8 citation statements)

References 6 publications

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“…These results are consistent with previous studies using 2-phenoxyethanol in teleost fishes [7,[11][12][13][14]27]. The effective anaesthetic concentration of 2-phenoxyethanol in a number of fish species has been reported ranging between 200 and 600 lL −1 [7,10,11,13,27]. At 500 lL −1 of 2-phenoxyethanol, the time to reach stage IV of induction (173 ± 7 seconds) was significantly differentfrom all other concentrations at 331 ± 15 seconds in 400 lL −1 and 143 ± 24 seconds in 600 lL −1 (Table 2).…”

Section: Resultssupporting

confidence: 93%

Efficacy of 2-Phenoxyethanol as an Anaesthetic for Adult Redline Torpedo Fish,Sahyadria denisonii(Day 1865)

Sajeevan

2014

International Journal of Zoology

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Efficacy of 2-phenoxyethanol for redline torpedo fish exposed to five concentrations (200, 300, 400, 500, and 600 lL −1 ) was evaluated. The time periods necessary for each characteristic stage of induction and recovery were recorded. Results indicated that the induction time of the fish exposed to five anaesthetic concentrations significantly ( < 0.05) decreased with increasing concentration but recovery time was independent of concentration. Concentration of 500 lL −1 (induction time: 173 ± 7 and recovery time: 129 ± 41 seconds) was determined as the minimum effective concentration that induces anaesthesia in less than 3 minutes.

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Section: Resultssupporting

confidence: 93%

Efficacy of 2-Phenoxyethanol as an Anaesthetic for Adult Redline Torpedo Fish,Sahyadria denisonii(Day 1865)

Sajeevan

2014

International Journal of Zoology

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“…Although some researchers have already described techniques to minimize the stress effects on matrinxã Roubach et al 2001;Urbinati and Carneiro 2001;Inoue et al 2002;Inoue et al 2004;Urbinati et al 2004;Inoue et al 2005), their application can only minimize the stress responses during the stressor agent and, consequently, the mortality rates. Therefore, the adverse effects of the stress responses after stressor agents are still an issue and must be overcome.…”

Section: Introductionmentioning

confidence: 99%

LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus

et al. 2011

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In order to determine the lethal dose (96-h LD 50 ) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutions: T 1 -Control (0.9% NaCl saline solution); T 2 (4 x 10 11 cells/ mL); T 3 (5 x 10 11 cells/ mL); T 4 (1.36 x 10 12 cells/ mL) and T 5 (3.06 x 10 12 cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L -1 ), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD 50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T 1 = 0%; T 2 = 16.66%; T 3 = 44.44%; T 4 = 72.22% and T 5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD 50 value of A. hydrophila to matrinxã is 6.66 x 10 11 cells/ mL. Keywords: Aeromonas hydrophila, 96-h LD 50 , matrinxã, tropical fishes DL 5o da bactéria Aeromonas hydrophila para o matrinxã, Brycon amazonicus resumo Para determinar a dose letal (DL 50 96-h) da bactéria Aeromonas hydrophila para o matrinxã, Brycon amazonicus, com aplicabilidade para testes de desafio, foram utilizados 90 peixes (63,23 ± 6,39 g), divididos em cinco tratamentos, com diferentes soluções bacterianas: T 1 -Controle (solução salina 0,9% NaCl); T 2 (4 x 10 11 células/ mL); T 3 (5 x 10 11 células/ mL -1 ); T 4 (1,36 x 10 12 células/mL -1 ) e T 5 (3,06 x 10 12 células/ mL -1 ). Os peixes foram previamente anestesiados com benzocaína (60 mg L -1 ), inoculados na cavidade peritoneal com as suspensões bacterianas e distribuídos em 15 aquários de vidro de 80 L de capacidade, com aeração constante. O experimento teve duração de 96 h, no qual foram monitoradas a mortalidade e a qualidade da água. O delineamento experimental foi inteiramente casualisado com três réplicas e a DL 50 96-h foi estimada de acordo com o método Spearman-Karber. Durante o experimento os parâmetros físico-químicos da água permaneceram dentro das condições consideradas adequadas para o desenvolvimento e saúde dos organismos aquáticos. A mortalidade dos peixes aumentou nas concentrações crescentes de A. hydrophila (T 1 = 0%; T 2 = 16,66%; T 3 = 44,44%; T 4 = 72,22% e T 5 = 100%), contudo, as primeiras mortalidades ocorreram em 57 h após a inoculação das concentrações bacterianas, sendo observados os primeiros sinais de infecção em 24 h após a inoculação. Os resultados indicam que o valor da DL 50 96-h da bactéria A. hydrophila para o matrinxã foi igual a 6,66 x 10 11 células/mL de solução salina. PAlAvrAs-chAve: Aeromonas hydrophila, DL 50 96-h, matrinxã...

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“…A major drawback of 2-phenoxyethanol is that it requires rather high anaesthetic doses in fi shes. The range of effective anaesthetic doses of 2-phenoxyethanol in most fi sh species are from about 0.20 to 0.60 ml·l -1 (Inoue et al 2004). 2-phenoxyethanol, which despite of some secondary negative effects (Ortuño et al 2002), its questionable usefulness (Kaiser and Vine 1998) and potential hazard to the 490 Groups with different alphabetic superscripts differ signifi cantly at p < 0.05 (ANOVA).…”

mentioning

confidence: 99%

Effects of 2-phenoxyethanol Anaesthesia on Haematological Profile on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss)

2007

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Velíšek J., Z. Svobodová, V. Piačková: Effects of 2-Phenoxyethanol Anaesthesia on Haematological Profi le on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss). Acta Vet. Brno 2007, 76: 487-492.The aim of this study was to assess changes in haematological profi le of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) after the exposure to anaesthetic 2-phenoxyethanol. The haematological profi le was assessed before, immediately after 10 min of anaesthesia and 24 h after the anaesthesia with recommended concentration of 0.30 ml·l -1 2-phenoxyethanol. The 10-min exposure to 2-phenoxyethanol of common carp caused the signifi cant increase (p < 0.01) in the haematocrit value and relative and actual count of monocytes immediately after the anaesthesia. These values returned back to normal within 24 hours. In rainbow trout, 2-phenoxyethanol anaesthesia had no effect on the haematological profi le. Results of the examinations suggest that the use of 2-phenoxyethanol at the concentration of 0.30 ml·l -1 does not cause irreversible damage of the blood in common carp and rainbow trout.Anaesthetic, erythrocyte profi le, leukocyte profi le, fi sh

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Standardization of 2-phenoxyethanol as anesthetic for juvenile Brycon cephalus (Gunther, 1869): the use in field procedures

Abstract: Hyper motility is a negative factor in fish handling

Cited by 9 publications

References 6 publications

Efficacy of 2-Phenoxyethanol as an Anaesthetic for Adult Redline Torpedo Fish,Sahyadria denisonii(Day 1865)

Efficacy of 2-Phenoxyethanol as an Anaesthetic for Adult Redline Torpedo Fish,Sahyadria denisonii(Day 1865)

LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus

Effects of 2-phenoxyethanol Anaesthesia on Haematological Profile on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss)

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