Efficacy of 2-Phenoxyethanol as an Anaesthetic for Adult Redline Torpedo Fish,<i>Sahyadria denisonii</i>(Day 1865)

We studied the simultaneous effect of sex and dose on anaesthesia efficacy to estimate, if possible, the lowest effective dose (LED) for clove oil, tricaine methanesulphonate (MS-222), 2-phenoxyethanol (2-PE) and propofol in mature guppies. LED is the lowest dose needed to reach A5 stage in a mean time of 3 min, with mean recovery (R5) time of 5 min. We used four doses/anaesthetic: 25, 50, 75 and 100 mg/L for clove oil; 120,140,160 and 180 mg/L for MS-222; 800, 1,000, 1,200 and 1,400 mg/L for 2-PE, and 7.50, 8.25, 10.00 and 11.25 mg/L for propofol. Each dose was tested on 10 females and 10 males. Morbidity, mortality and behavioural changes were checked on two control groups (10 males and 10 females/group). Sedation (A3), A5 and R5 times were recorded. Significant interactive effect dose*sex on A5 time was found for each anaesthetic agent (p dose*sex < .05). Except for MS-222 (p dose*sex = .284), significant interactive effect dose * sex on R5 time was found (p dose*sex < .05). A5 time in females tended to be greater than in males, but, in general, R5 times were longer in males. Body size differences between males and females could explain these differences in MS-222 on A5 time and for clove oil, 2-PE and propofol on R5 time. No dose simultaneous meet LED 0 s conditions relating to both A5 and R5 times; therefore the lowest doses inducing A5 in a mean time of 3 min could be a safe guideline for anaesthetic procedure in both male and females.

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The Efficacy of Clove Seed Extracts as an Anaesthetic Agent and Its Effect on Haematological Parameters of African Catfish (Clarias Gariepinus)

Akinrotimi¹

2015

Int J Aquac Fish Sci

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Background and Aim: The intensive nature of aquaculture has subjected fish to a number of stressors in the culture medium, anesthetics are widely used to minimize the issue of stress during farming operations and activities. Clove oil is a well known, established and acceptable anesthetics commonly used in aquaculture, this anesthetics is not readily available in developing countries, thus leaving the fish farmers with option of using the locally available plant extracts as anesthetic agent in fish culture. This study therefore assessed the efficacy of aqueous extracts of clove seed and its effect on hematological parameters of the fish.Methods: A total of 300 Clarias gariepinus comprises of 150 juveniles and 150 adult fish were exposed in triplicates to aqueous extracts of clove seed at concentrations of 5.0, 10.0, 15.0, 20.0 and 25.0mg/l at the rate of 10 fish per tank for both juveniles and adult fish. Induction time (Time taken for the fish to be completely anaesthetized) and recovery time (Time taken for the fish to resume normal swimming) was monitored using stop watch. Also, the effect of the extracts on water quality, survival and hematological parameters of the fish were evaluated using standard methods. Results:The result obtained revealed that the induction time indicated a size related response with the adult fish significantly (P<0.05) higher than the juveniles at all concentration of exposure. However, reverse was the case in recovery time, as the recover times in juveniles were higher than the adult fish. The survival was 100% in both sizes. Significant alterations were recorded in the haematological variables of the fish, which was more pronounced at higher concentrations of the extracts.

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Efficacy of 2-Phenoxyethanol as an Anaesthetic for Adult Redline Torpedo Fish,Sahyadria denisonii(Day 1865)

Cited by 3 publications

References 23 publications

Concentration effects of three common fish anesthetics on Pacific hagfish (Eptatretus stoutii)

Concentration effects of three common fish anesthetics on Pacific hagfish (Eptatretus stoutii)

Simultaneous effect of sex and dose on efficacy of clove oil, tricaine methanesulfonate, 2-phenoxyethanol and propofol as anaesthetics in guppies,Poecilia reticulata(Peters)

The Efficacy of Clove Seed Extracts as an Anaesthetic Agent and Its Effect on Haematological Parameters of African Catfish (Clarias Gariepinus)

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