Spleen autotransplantation in mice: A novel experimental model for immunology study

Miko, Iren; Bráth, Endre; Furka, I.; Kovács, Judit; Kelvin, David J.; Zhong, Robert

doi:10.1002/micr.1026

Cited by 21 publications

(29 citation statements)

References 8 publications

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“…The mouse model has certain advantages, including genetic homogeneity, which reduces individual differences, as well as decreased costs and fewer staffing requirements. 8,9 We previously reported that spleen autotransplantation restores most of the spleen's hematological and immunological functions. 9 As these studies were conducted 6 weeks after splenectomy and spleen transplantation, the long-term consequences of this procedure remain unknown.…”

Section: à9mentioning

confidence: 99%

Hemorheological follow‐up after splenectomy and spleen autotransplantation in mice

et al. 2006

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We previously reported on a spleen autotransplantation model in mice, with spleen function studies at 6 weeks. The present study was undertaken to investigate long-term hemorheological functions. A/J and BALB/c inbred mice were divided into four groups: control, sham surgery (SH), splenectomy (SE), and spleen autotransplantation (AU). Hematological and hemorheological parameters were determined. Leukocyte counts in the SE and AU groups were significantly higher than in controls, while hematocrit levels were markedly lower. Mean erythrocyte volume did not change significantly. Platelet counts in the AU group were significantly lower, and red blood cell deformability was significantly worse in the SE group. The AU group also had increased cell transit time, but it was less than that in the SE group. The SE group showed the highest fibrinogen levels. We conclude that there are some consistent differences in hematological parameters between splenectomy and spleen autotransplantation. These data suggest that spleen autotransplantation may partially restore hemorheological functions following splenectomy.

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Section: à9mentioning

confidence: 99%

Hemorheological follow‐up after splenectomy and spleen autotransplantation in mice

et al. 2006

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“…3,12 In this study autogenous spleen grafts were prepared in the retroperitoneum and the results compared with those at sites more commonly used (IO and FPS). All animals that had undergone splenic autotransplantation had gross evidence of viable splenic implants ( Fig.…”

Section: Autogenous Splenic Implantsmentioning

confidence: 99%

Antibody Response of Autogenous Splenic Tissue Implanted in the Abdominal Cavity of Mice

et al. 2005

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There is still controversy about the immunologic function of autotransplanted splenic tissue. In this study, splenic autotransplantation was performed in the abdominal cavity of mice, and the plaqueforming cell (PFC) assay was used to investigate the frequency of antibody-forming cells in response to sheep red blood cell (SRBC) immunization. BALB/c mice were divided into four groups according to the location of the autogenous graft: intraomental (IO), free peritoneal splenosis (FPS), retroperitoneal (RP), and nongrafted control (CT). Thirty days after surgery the mice were immunized intraperitoneally with SRBCs, and 4 days later splenic immunoglobulin M anti-SRBC-secreting cells were determined by counting the number of PFCs. All the immunized mice showed increased numbers of PFCs that were about 2 logs higher than those in the the nonimmunized controls (P < 0.005). The frequencies of anti-SRBC-producing cells in the tissues grafted in various sites of the abdominal cavity (IO, FPS, RP), in the normal spleen from nonoperated controls (CT), or in the sham-operated control group (SCT) were not notably different (5582 -2475 PFC/10 7 cells for IO; 4849 -1856 for FPS; 6604 -2903 for RP; 5940 -5029 for CT; and 6172 -2203 for SCT). Similar histology with small architectural variations was observed in all implants; less white pulp was involved, and there was more congestion in the red pulp, with extensive sinusoids and reticular fiber proliferation. This study shows that the T cell-dependent antibody response in implanted splenic tissues is as efficient as in the intact spleen, with no difference between the graft sites studied. This immune response does not depend on the slight architectural variations observed in the splenic implants.T he importance of the splenic autotransplant technique is justified by the high incidence of total splenectomy, the spleen being the most affected organ during blunt trauma as well as highly susceptible to iatrogenic incidents. 1,2 Since the nineteenth century splenectomy has been the treatment advised after splenic trauma, although current studies indicate that this procedure is not exempt from complications.

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“…9,10 Our study compared the multiple organ-chip model to a single liver, kidney, spleen, and intestine chip technique. The histological data revealed no difference: The spleen appeared to be the most preserved organ in our experimental model, as it had a recognizable structure without significant damage.…”

Section: Discussionmentioning

confidence: 99%

Multiorgan transplantation with a new organ‐chip technique in mice: Preliminary histological data

et al. 2003

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A simple model was developed for multiorgan liver-kidney-spleen-intestine transplantation on 108 inbred mice. Donor operations included hepatectomy, nephrectomy, splenectomy, and jejunum segment resection. Following removal of the organ, small slices or abdominal organ "chips" were prepared. During multiorgan recipient operations, chips from each of these organs were transplanted into the omentum; in the control single-organ groups, only 1 organ was transplanted. All animals survived. Biopsies were taken for histology after 6 weeks. All organs were found to have developed a blood supply. In the liver chips, hypertrophied cells could be detected. In the margin of the kidney tissue, both the glomeruli and tubules were preserved. Lymphoid zone and red pulp were intact in spleen chips. All layers of the intestinal chips were identifiable and contained intraluminal mucinous substances. This model is a simple surgical intervention with the possibility of the investigation of 4 organs.

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Spleen autotransplantation in mice: A novel experimental model for immunology study

Cited by 21 publications

References 8 publications

Hemorheological follow‐up after splenectomy and spleen autotransplantation in mice

Hemorheological follow‐up after splenectomy and spleen autotransplantation in mice

Antibody Response of Autogenous Splenic Tissue Implanted in the Abdominal Cavity of Mice

Multiorgan transplantation with a new organ‐chip technique in mice: Preliminary histological data

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