Hemorheological follow‐up after splenectomy and spleen autotransplantation in mice

Miko, Iren; Németh, Norbert; Sipka, Sándor; Bráth, Endre; Pető, Károly; Gulyás, Attila; Furka, I.; Zhong, Robert

doi:10.1002/micr.20208

Cited by 15 publications

(16 citation statements)

References 9 publications

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“…The median fibrinogen concentration in the HS patients with prior splenectomy (335 mg/dl) was at a level which has previously been associated with a twofold increased incidence of vascular events compared to subjects with fibrinogen level in the lowest tertile [41]. Elevated fibrinogen concentrations following splenectomy have previously been reported in mice [42], although other studies in human subjects have failed to show a significant difference in fibrinogen concentrations pre- and post-splenectomy in various conditions [43-45]. To our knowledge no prior studies have specifically examined patients with HS.…”

Section: Discussionmentioning

confidence: 99%

Traditional laboratory measures of cardiovascular risk in hereditary spherocytosis

Crary

Troendle

Ahmad

et al. 2010

Pediatric Blood & Cancer

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Background Individuals who have undergone splenectomy may be at an increased risk of arterial and venous thrombosis. We sought to determine if splenectomy affects surrogate laboratory measures of cardiovascular risk in persons with hereditary spherocytosis (HS). Procedure We conducted a prospective cross-sectional study of 21 children and 36 adults with HS. Fasting blood samples were collected for complete blood count and plasma lipid panel, homocysteine, lipoprotein (a), C-reactive protein, and fibrinogen. The variables were compared between the groups with and without prior splenectomy by Mann–Whitney tests. Results Subjects with prior splenectomy had higher hemoglobin, white blood cell and platelet counts and lower reticulocyte counts and total serum bilirubin concentrations (P < 0.001). Subjects not having had splenectomy had lower than normal levels of total and LDL-cholesterol (LDL-C). Total and LDL-C values were significantly higher, as were fibrinogen and homocysteine concentrations, in the post-splenectomy subjects than in individuals with intact spleen. Conclusion Various lipid levels and other measures of cardiovascular risk are affected by splenectomy in persons with HS. Further investigations are indicated to more clearly define the balance of the potential benefits of hemolysis and anemia versus the deleterious effects of splenectomy in HS.

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Section: Discussionmentioning

confidence: 99%

Traditional laboratory measures of cardiovascular risk in hereditary spherocytosis

Crary

Troendle

Ahmad

et al. 2010

Pediatric Blood & Cancer

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“…Likewise, other studies have revealed that the presence of Howell-Jolly-bodies was clearly reduced with increased regeneration and splenosis rate of auto-transplantation splenic fragments (14,15). The OPSI is reported to be the most important and frequently fatal complication of total splenectomy.…”

Section: Discussionmentioning

confidence: 90%

Auto-Transplantation of Splenic Fragments After Total Splenectomy in Patients with Severe Splenic Trauma Lesions: A Clinical Study

Khorshidi¹,

Kasraianfard²,

Ghaderzadeh³

et al. 2017

Trauma Mon

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Background: The known early and late complications following splenectomy increase the tendency to preserve splenic tissue after splenic trauma. Objectives: This study was conducted to determine the safety and feasibility of auto-transplantation of splenic fragments in patients with severe splenic injury. Methods: Nineteen patients with severe splenic blunt trauma, who underwent total splenectomy and autotransplantation of splenic fragments at Besat hospital, Hamadan University of Medical Sciences, Iran during years 2015 and 2016, were enrolled in the study. Complete blood counts, blood smear for Howell-Jolly bodies, and phagocytic function tests were performed just before, at 3 months, and 6 months after surgery. Results: The means of red blood cell counts increased significantly at both 3 and 6 months after surgery (P = 0.01 and 0.049, respectively) and the means of hemoglobin, hematocrit, and the percentage of lymphocytes increased significantly at 3 months after surgery (P = 0.001, 0.046, and 0.01 respectively) while this increase was not significant 6 months after surgery (P = 0.52, 0.15, and 0.34, respectively). The Howell-Jolly bodies were present in 5 patients (26.3%) at 3 months after surgery, which was significantly reduced

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“…spleen chips group could be related to the fact that not all of the spleen transplants were found in these experimental animals. They were supposedly in "functional hyposplenic state" at that time 17,26 . In the animal autotransplanted with 10 spleen chips the scintigraphy showed activity in the region of the greater omentum, confirming the phagocytic function of the autotransplants.…”

Section: Diagnostic Laparoscopymentioning

confidence: 99%

Long-term following-up of viability of spleen autotransplants in the Beagle canine model

et al. 2012

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PURPOSE: To examine the possible late complications of splenectomy or spleen autotransplantation in large laboratory animal model, in which we need non-invasive or minimal-invasive methods for long-term monitoring of the experimental animals. METHODS: Experimental groups of beagle dogs were: non-operated control, sham-operated control, splenectomy, spleen autotransplantation with 5 or 10 spleen-chips taken into the greater omentum (Furka's technique). Prior to operations, on the 1st postoperative week, monthly till the 6th as well as in the 9th and 12th month, hemorheological examinations were performed. In postoperative 12th month colloid scintigraphy and diagnostic laparoscopy were carried out. At the end of the investigation comparative morphological examinations were performed, too. RESULTS: From the 4th-5th postoperative month filtration function of spleen-autotransplants showed particular restoration compared to splenectomy group. However, the functional results did not reach the values of the control or sham-operated groups. Sham-operated control's scintigraphy nicely showed activity in the spleen. In spleen autotransplantation-groups scintigraphy indicated well the activity of spleen-chips. During diagnostic laparoscopy spleen-chips with their blood supply were found. Histologically, the structure of spleenautotransplants was similar to normal splenic tissue. CONCLUSIONS: The autotransplants are regenerated, their functions have been partly restored, and thus spleen autotransplantation may prevent the possible complications of splenectomy. These parameters and the presented investigative protocol are suitable for long-term following-up of viability of the spleen-autotransplants.

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Hemorheological follow‐up after splenectomy and spleen autotransplantation in mice

Cited by 15 publications

References 9 publications

Traditional laboratory measures of cardiovascular risk in hereditary spherocytosis

Traditional laboratory measures of cardiovascular risk in hereditary spherocytosis

Auto-Transplantation of Splenic Fragments After Total Splenectomy in Patients with Severe Splenic Trauma Lesions: A Clinical Study

Long-term following-up of viability of spleen autotransplants in the Beagle canine model

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