Sperm impairments in adult vesper mice (Calomys laucha) caused byin uteroexposure to bisphenol A

Abstract

This study aimed to evaluate the effects of in utero administration of bisphenol A (BPA) on semen parameters of vesper mice. Sixty female Calomys laucha were divided into six groups and received by gavage during gestation the following substances: Water (negative control), Olive Oil (vehicle control), Diethylstilbestrol (DES - positive control - 6.5 μg kg(-1) bw) and BPA (40, 80 and 200 μg kg(-1) bw). Male offspring were euthanised at 70 days of age, and sperm parameters were analysed. BPA reduced normal sperm morphology (water = 96.1 ± 0.65; BPA200 = 96.8 ± 2.3%), sperm membrane integrity (water = 88.8 ± 1,65; BPA200 = 70.6 ± 4,15%), sperm motility (water = 87.5 ± 1.71; BPA200 = 51.3 ±9.9%) and in vitro penetration rates (water = 55.0 ± 7.14; BPA200 = 7.47 ±2.96%), but it did not affect body weight, anogenital distance, sperm DNA integrity and acrosome integrity. In conclusion, in utero exposure to BPA caused a reduction in sperm parameters of adult C. laucha. Natural mating studies should be conducted to verify the effects of BPA on fertility of the animals.

Bisphenol-A Affects Male Fertility via Fertility-related Proteins in Spermatozoa

“…1A, B ). Similar effects of BPA on sperm motility have been shown in rat 4 and mice 9 in vivo ; however, in vitro exposure data on mammalian species have not been reported. Accordingly, decreased motility after exposure to 100 μM BPA was associated with decreased ATP levels in spermatozoa ( Fig.…”

“…Most studies on BPA in the context of male reproduction/fertility have been conducted in vivo , and these studies have consistently reported its developmental toxicity to spermatozoa via DNA damage 4 , DNA methylation 5 , oxidative stress 6 , and lipid peroxidation 7 , among other mechanisms. While few in vivo studies have shown an association between BPA and spermatozoa functional parameters, BPA has been linked to changes in motility 8 , morphology 9 , and intracellular ATP content 6 .…”

Evaluation of subacute bisphenol – A toxicity on male reproductive system

“…Motility is a necessary function for sperm transport through the male and female reproductive tract. In this study, motility was significantly reduced in the BPA-treated groups compared with control groups, suggesting that BPA might have altered the mitochondrion, producing delay in motility and in some cases, eventually leading to sperm death resulting in reduced total sperm count [ 26 , 27 ]. The explanation given for the reduced total sperm count and reduced sperm motility were again possible reasons for increased dead sperm counts due to BPA toxicity in the present study.…”

“…Studies focusing on exposure to environmental factors (pesticides, phthalates, PCBs, air pollution, trihalomethanes, mobile phones) on semen quality by some worker revealed low sperm concentration, sperm motility, and also abnormal sperm morphology [ 25 , 26 ].…”

Endocrine disruptive compounds and male reproduction

“…The groups with the highest concentrations also showed decreased motility of sperm cells. In all the groups of males exposed to BPA, fertilizing ability in the in vitro system was significantly decreased [17].…”