Aim:The aim was to evaluate the effect of multiple oral administration of bisphenol A (BPA) for 28 days on seminal characteristic on mammal using Wistar rat as a model.Materials and Methods:Rats were randomly divided into five different groups having 6 male rats in each group. The doses chosen were 50, 200, and 600 mg/kg body weight for Groups III, IV and V, respectively, based on preliminary dose range finding study and Group II served as vehicle control and Group I was negative control.Results:Reproductive study in the BPA-treated rats on day 28 revealed that there was significant (p≤0.05) reduction in the epididymal sperm count of rats of Group IV and significant (p≤0.01) decrease in Group V. Sperm motility percentage, dead count percentage, head and tail abnormality percentage were found to be significantly (p≤0.01) increased in rats of BPA-treated groups as compared to rats of control groups. Testes showed necrosis of germinal layer and spermatogonial cells in the seminiferous tubules. Hematological examination revealed significant (p≤0.01) decrease in the mean values of total erythrocyte count (TEC), total leukocyte count (TLC), hemoglobin, packed cell volume, and there was also significant (p≤0.05) lymphocytopenia in treated animals.Conclusion:It can be concluded from this study that subacute toxicity of BPA caused a reduction in the epididymal sperm count, sperm motility, dead count, head and tail abnormality, as well as hematological indices such as TLC, TEC etc. Hence, it appears that BPA affects the germ cells leading to impairment in the spermatogenesis, and thus having its property as reproductive toxicant and it also suppresses bone marrow functioning, which leads to normocytic hypochromic anemia in rats.

To see the toxicopathological changes after multiple exposure to acetamiprid (ACP) and also to obtain more information regarding the manner in which ACP acts at cellular level. Materials and Methods:A subacute toxicity study of ACP was undertaken in 72 female Wistar rats in four groups (18 each). Three different concentrations of ACP (25, 100 and 200 mg/kg of body weight) were administered orally to rats. Untreated rats served as control. Different plasma enzyme and analytes were measured. Gross and histopathological observations were noted in this experiment.Result: There was a significant increase in the plasma enzymes tested in this experiment. There was a significant decrease in plasma glucose, cholesterol and low-density lipid. Necrotic and degenerative changes were observed in vital organs. Conclusion:It is observed that ACP has the toxic potential (on liver, kidney, heart, ovary and brain) at sub lethal doses.

No abstract