Spectrophotometric determination of cephalexin in dosage forms with imidazole reagent

Al–Warthan, Abdulrahman; Fattah, S. Abd El; Zahran, N M

doi:10.1016/0039-9140(92)80085-r

Cited by 21 publications

(8 citation statements)

References 8 publications

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“…The potency of antibiotics can be determined by chemical and biological methods. Chemical methods such as capillary electrophoresis, ultraviolet spectrophotometry, high performance liquid chromatography and high performance thin layer chromatography have been used for the quantitative determination of Cephalexin in formulations as well as in human urine, and serum 11 . However, the microbiological assay for determination of potency of Cephalexin has not yet been reported in any pharmacopoeia.…”

Section: Figure 1: Cephalexin Structurementioning

confidence: 99%

Development and Validation of Microbial Bioassay for Quantification of Cephalexin in Pharmaceutical Preparations

Reddy¹,

Dagar²,

Ramesh³

2017

Int. Res. J. Pharm.

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The aim of this study was to develop and validate a simple, sensitive, precise and cost-effective one-level agar diffusion (5+1) bioassay for estimation of potency and bioactivity of Cephalexin in pharmaceutical preparation which has not yet been reported in any pharmacopoeia. Escherichia coli MTCC-443 was selected as the most significant strain against Cephalexin. Bioassay was optimized by investigating several factors such as buffer pH, inoculums concentration and reference standard concentration. Identification of Cephalexin in commercial sample Cephadex tablet was done by FTIR spectroscopy. Mean potency recovery value for Cephalexin in Cephalexin tablet was estimated as 100.2%. A validated bioassay method showed linearity (r 2 =0.999), precision (Intraday RSD=1.09%, and Interday RSD=0.94%) and accuracy (99.53%, RSD=0.306%). Bioassay was correlated wi th HPLC using same sample and estimated potencies were 100.2% and 99.25%, respectively. Results show that bioassay is a suitable method for estimation of potency and bioactivity of Cephalexin pharmaceutical preparations.

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Section: Figure 1: Cephalexin Structurementioning

confidence: 99%

Development and Validation of Microbial Bioassay for Quantification of Cephalexin in Pharmaceutical Preparations

Reddy¹,

Dagar²,

Ramesh³

2017

Int. Res. J. Pharm.

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“…According to literature surveys, there are different analytical methods reported for the determination of CF, CT. It includes UV-Visible spectroscopy [3][4][5][6][7][8][9][10][11][12], chemiluminescence [13], near infrared spectroscopy [14], potentiometry [15], polarography [16,17], HPLC [18][19][20][21][22][23][24][25][26], gel filtration chromatography [27], HPLC [28], capillary zone electrophoresis [29], LC-MS [30,31], and MS [32] methods. But no analytical methods are reported for the determination of CF, CT in dry syrup formulation using the mobile phase Triethylamine: Methanol: Acetonitrile: Ultra Pure Water (2: 10: 20: 68 v/v%) by HPLC (High Performance liquid chromatography).…”

Section: Cefixime Trihydrate (Ct): (6r7r)-7-{[(2r)-2-amino-2-phenylmentioning

confidence: 99%

Determination of Cefuroxime Axetil and Cefixime Trihydrate in Pharmaceutical Dosage Forms by RP-HPLC Method

Elias¹,

Alfeen²

2016

Pharm Anal Chem

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A High Performance liquid chromatographic method was developed for the determination of Cephalosporins like as: Cefuroxime axetil, Cefixime trihydrate, in pharmaceutical dosage forms. Isocratic separation was performed on an Enable C18 column (250 mm x 4.6 mm, 5.0µm) using Triethylamine: Methanol: Acetonitrile: Ultra Pure Water (2: 10: 20: 68 v/v%) as the mobile phase at a flow rate of 1.0 ml/min. The PDA detection wavelength was set at 265nm. The linearity was observed over a concentration range of 0.1-80 µg/ml for HPLC method (correlation coefficient=0.999). The developed methods were validated according to ICH guidelines. The relative standard deviation values for the method precision studies were < 2%, and the accuracy was > 99%. The developed method was used successfully for the determination of Cefuroxime axetil, Cefixime trihydrate, in Capsule, Tablet and dry syrup formulations.

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“…Several methods have been reported for the determination of imidazole, 4-methylimidazole, and 2-methylimidazole that include spectrophotometry (Garfinkel and Edsall 1958;Alwarthan, Fattah, and Zahran 1992), gas chromatography (Wilks et al 1973;Fuchs and Sundell 1975;Guo et al 2005), high performance liquid chromatography (Wilks, Johnson, and Shingler 1977;Karangwa, Mitchell, and Tucker 1990), gas chromatography-mass spectrometry(Moore-Testa, Saint-Jalm, and Testa 1984; Charlton and Jones 2007), and liquid chromatography tandem mass spectrometry (Yamaguchi and Masuda 2011;Schlee et al 2013). …”

Section: Introductionmentioning

confidence: 98%

Determination of Imidazole, 4-Methylimidazole, and 2-Methylimidazole in Cigarette Additives by Ultra-High Performance Liquid Chromatography

et al. 2015

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Ultra-high performance liquid chromatography was employed for the determination of imidazole, 4-methylimidazole, and 2-methylimidazole in cigarette additives. Following solid phase extraction and filtration, the analytes were separated using isocratic elution with 5 mmol/L acetonitrileammonium formate (80:20, v/v) at 0.5 mL/min. The quantification of these analytes was achieved with the external standard method on a diode-array detector at 215 nm. Baseline separation was achieved within 1.3 min. The linear dynamic ranges for imidazole, 4-methylimidazole, and 2-methylimidazole were between 0.0375 and 18.0300 mg/kg. The limits of detection for the analytes were 0.0094 mg/kg. The recoveries and the relative standard deviations at fortification levels of 0.1322 mg/kg to 1.6220 mg/kg were 95.20% to 101.93% and 0.55% to 2.54%, respectively. The method offers easy operation, rapid analysis, and accurate results, and is suitable for the determination of imidazole, 4-methylimidazole and 2-methylimidazole in cigarette additives.

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Spectrophotometric determination of cephalexin in dosage forms with imidazole reagent

Cited by 21 publications

References 8 publications

Development and Validation of Microbial Bioassay for Quantification of Cephalexin in Pharmaceutical Preparations

Development and Validation of Microbial Bioassay for Quantification of Cephalexin in Pharmaceutical Preparations

Determination of Cefuroxime Axetil and Cefixime Trihydrate in Pharmaceutical Dosage Forms by RP-HPLC Method

Determination of Imidazole, 4-Methylimidazole, and 2-Methylimidazole in Cigarette Additives by Ultra-High Performance Liquid Chromatography

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