Determination of Cefuroxime Axetil and Cefixime Trihydrate in Pharmaceutical Dosage Forms by RP-HPLC Method

Abstract

A High Performance liquid chromatographic method was developed for the determination of Cephalosporins like as: Cefuroxime axetil, Cefixime trihydrate, in pharmaceutical dosage forms. Isocratic separation was performed on an Enable C18 column (250 mm x 4.6 mm, 5.0µm) using Triethylamine: Methanol: Acetonitrile: Ultra Pure Water (2: 10: 20: 68 v/v%) as the mobile phase at a flow rate of 1.0 ml/min. The PDA detection wavelength was set at 265nm. The linearity was observed over a concentration range of 0.1-80 µg/ml for HPLC method (correlation coefficient=0.999). The developed methods were validated according to ICH guidelines. The relative standard deviation values for the method precision studies were < 2%, and the accuracy was > 99%. The developed method was used successfully for the determination of Cefuroxime axetil, Cefixime trihydrate, in Capsule, Tablet and dry syrup formulations.

Evaluation of Different Brands of Cefixime Available in Pakistan by Newly Developed Spectrophotometric Method

“…[2,3]. CFX is thought to be used for drug resistant bacteria [4]. Due to the frequent prescribing of CFX, it is necessitated to analyze from dosage form and from biological fluids as well.…”

Determination of cefixime in pure form, in pharmaceutical products and biological samples through fluorescence quenching of eosin Y

“…Recent and comparative literature studies have been described for assay of CFX such as HPTLC, [ 9,10 ] spectrophotometry, [ 11–14 ] RP‐HPLC, [ 15,16 ] HPLC–MS, [ 8 ] HPLC‐UV, [ 17 ] and electrocatalytic oxidation. [ 18 ] An LC–MS/MS technique has been reported that quantifies CFX in human plasma utilizing cefetamet as the internal standard.…”

Direct Spectrophotometric Determination of Cefuroxime Axetil in Pure Form and Pharmaceutical Dosage Forms.

“…Various spectrophotometric [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22], High Performance Liquid Chromatographic (HPLC), Reversed-phase high performance liquid chromatography (RP-HPLC), high performance thin layer chromatographic (HPTLC), micellar liquid chromatographic (MLC) [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] and voltammetric [38,39] methods have been reported for the determination of cefuroxime.…”