A High Performance liquid chromatographic method was developed for the determination of Cephalosporins like as: Cefuroxime axetil, Cefixime trihydrate, in pharmaceutical dosage forms. Isocratic separation was performed on an Enable C18 column (250 mm x 4.6 mm, 5.0µm) using Triethylamine: Methanol: Acetonitrile: Ultra Pure Water (2: 10: 20: 68 v/v%) as the mobile phase at a flow rate of 1.0 ml/min. The PDA detection wavelength was set at 265nm. The linearity was observed over a concentration range of 0.1-80 µg/ml for HPLC method (correlation coefficient=0.999). The developed methods were validated according to ICH guidelines. The relative standard deviation values for the method precision studies were < 2%, and the accuracy was > 99%. The developed method was used successfully for the determination of Cefuroxime axetil, Cefixime trihydrate, in Capsule, Tablet and dry syrup formulations.
This study presents a chromatographic analysis method for the determination of mefenamic acid in pharmaceutical preparations and treated wastewater from the pharmaceutical industry in East Asia and specifically (Syria). An isocratic RP-HPLC method has been developed for determination of Mefenamic acid on a Grace, alltima C18 column (250 x 4.6 mm, 5.0 μm) using a mobile phase consisting of (1%) Triethylamine aqueous buffer adjust pH = 2 by H3PO4 (85%): Methanol: Acetonitrile); (35: 20: 45 v\v\v %) at a flow rate of 2 mL/min. Detection was carried out at 220 nm. Retention time of Mefenamic acid was 7.85 ( ± 0.36) mins. The method was validated with respect to specificity, linearity, accuracy, precision, ruggedness, and robustness. The proposed method is simple, precise, sensitive, and reproducible and is applicable for quantification of Mefenamic acid in Tablets formulations and Wastewater developed and formulated in our laboratory.
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