Spectrophotometric Analysis of Valsartan and Hydrochlorothiazide

Abstract

Two new methods for the simultaneous determination of valsartan and hydrochlorothiazide in pharmaceutical dosage forms have been developed. The first method, based on compensation technique is presented for the derivative spectrophotometric determination of binary mixtures with overlapping spectra. By using ratios of the derivative maxima or the derivative minimum, the exact compensation of either component in the mixture can be achieved, followed by its determination. The second method, differential derivative spectrophotometry, comprised of measurement of the difference absorptivities derivatized in the first order (ÁD 1 ) of a tablet extract in 0.1 N NaOH relative to that of an equimolar solution in methanol at wavelengths of 227.8 and 276.5 nm, respectively. Neither sample pre-treatment nor separation were required. These methods showed good linearity, precision, and reproducibility. The results obtained were compared with the results of reversed phase HPLC. It was found that 283 ORDER REPRINTS the difference was not statistically important between these methods. The proposed methods were accurate, sensitive, precise, reproducible and could be applied directly and easily to the pharmaceutical preparations.

Furosemide Determination by First‐Derivative Spectrophotometric Method

“…In recent years, the literature has been showing an increase in the methods based on the derivative spectrophotometry. It allows enhancement of the resolution of overlapping spectra and selective discrimination of sharp bands over broad bands, thereby offering effective approaches to analysis of drug association and impurities (Erk and Gönüllü 2002;Malliou, Antoniou, and Koundourellis 2002;Erk 2002;Aboul-Eneim, Goger, and Turkalp 2002;Uslu and Ozkan 2002;Joseph-Charles et al 2003;Biryol and Erk 2003).…”

Spectrofluorimetric Assessment of Hydrochlorothiazide Using Optical Sensor Nano-Composite Terbium Ion Doped in Sol-Gel Matrix

“…These include electrochemical methods [1], Chemiluminescence methods [2], Capillary Zone Electrophoresis methods [3], Vierordt's method [4], spectrophotometric [4][5][6][7][8][9][10][11][12][13][14][15], HPLC [16][17][18][19][20][21], derivative spectroscopy methods [22][23][24]. High-Performance ThinLayer Chromatography [25], Liquid Chromatographic [25][26][27][28] and Spectral analysis methods [29][30][31][32][33].…”

Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form

“…There are some methods for simultaneous estimation of both drugs using HPTLC [8,9] and ion pair chromatography [10]. Some UV spectroscopic techniques are also reported in which simultaneous method, AUC method, and first derivative method were developed [11][12][13][14][15][16]. Simultaneous estimation of drug combination is generally done by separation using chromatographic methods like HPLC, GC and HPTLC, and so forth.…”