Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form

Abstract

Two UV-spectrophotometric methods have been developed and validated for simultaneous estimation of valsartan and hydrochlorothiazide in a tablet dosage form. The first method employed solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 249.4 nm and 272.6 nm, max for valsartan and hydrochlorothiazide, respectively. The second method was absorbance ratio method, which involves formation of Q-absorbance equation at 258.4 nm (isoabsorptive point) and also at 272.6 nm ( max of hydrochlorothiazide). The methods were found to be linear between the range of 5-30 g/mL for valsartan and 4-24 g/mL for hydrochlorothiazide using 0.1 N NaOH as solvent. The mean percentage recovery was found to be 100.20% and 100.19% for the simultaneous equation method and 98.56% and 97.96% for the absorbance ratio method, for valsartan and hydrochlorothiazide, respectively, at three different levels of standard additions. The precision (intraday, interday) of methods was found within limits (RSD < 2%). It could be concluded from the results obtained in the present investigation that the two methods for simultaneous estimation of valsartan and hydrochlorothiazide in tablet dosage form are simple, rapid, accurate, precise and economical and can be used, successfully, in the quality control of pharmaceutical formulations and other routine laboratory analysis.

Figure 15 from: Peleshok K, Bondar B, Kryskiw L, Kucher T, Poliak O, Logoyda L (2021) Development and Validation of Spectrophotometric Methods for Estimation of Valsartan in Tablet Dosage Form. Pharmacia 68(4): 852-858. https://doi.org/10.3897/pharmacia.68.e73559

“…The scientific literature describes methods for quantitative determination of valsartan by spectrophotometry -by intrinsic light absorption (Tatar and Sağlık 2002;Kumar et al 2011;Gupta et al 2010;Tajane 2018;Jadhav et al 2014;Lotfya et al 2015;Kamal et al 2020;Drapak et al 2019aDrapak et al , 2019b and by reaction products with different reagents (Ramachandran et al 2011;Omar et al 2011). Indian scientists have been developed spectrophotometric methods for the determination of valsartan and ezetimibe in drugs using sulfophthaleine dyes such as bromophenol blue and bromocresol green (Ramachandran et al 2011).…”

Figure 9 from: Peleshok K, Bondar B, Kryskiw L, Kucher T, Poliak O, Logoyda L (2021) Development and Validation of Spectrophotometric Methods for Estimation of Valsartan in Tablet Dosage Form. Pharmacia 68(4): 852-858. https://doi.org/10.3897/pharmacia.68.e73559

“…The scientific literature describes methods for quantitative determination of valsartan by spectrophotometry -by intrinsic light absorption (Tatar and Sağlık 2002;Kumar et al 2011;Gupta et al 2010;Tajane 2018;Jadhav et al 2014;Lotfya et al 2015;Kamal et al 2020;Peleshok et al 2021;Drapak et al 2019aDrapak et al , 2019b and by reaction products with different reagents (Ramachandran et al 2011;Omar et al 2011). Indian scientists have been developed spectrophotometric methods for the determination of valsartan and ezetimibe in drugs using sulfophthaleine dyes such as bromophenol blue and bromocresol green (Ramachandran et al 2011).…”

HPLC method transfer study for simultaneous determination of seven angiotensin II receptor blockers

“…Literature survey reveals that ARA‐II were determined either in mixtures or individually by different chromatographic techniques such as HPLC, LC‐MS/MS, CE, and derivative spectrophotometry [3–27].…”