Spectrofluorimetric analysis of certain macrolide antibiotics in bulk and pharmaceutical formulations

Khashaba, Pakinaz Y.

doi:10.1016/s0731-7085(01)00609-4

Cited by 58 publications

(26 citation statements)

References 26 publications

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“…AZI has been analyzed by spectrophotometric methods [3][4][5], high-perfor-mance liquid chromatography using fluorescence [6][7][8], electro-chemical using amperometric detection [9][10] and coulometric detection [11][12][13] and mass spectrometry detector [14][15][16][17] for quantification in bulk material and pharmaceutical dosage forms. Fluorescence detection requires complicated sample pretreatment involving pre-column derivatization of the analyte.…”

Section: Trideoxy-3-(dimethylamino)-β-d-xylo-]oxy}-1-oxa-6-azacyclopementioning

confidence: 99%

A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma

Younes

Elkady

2014

Eur. J. Chem.

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A simple, rapid and sensitive LC-MS/MS method was developed and validated for the quantification of azithromycin (AZI) in human plasma. Sildenafil citrate was used as an internal standard. The analytes were extracted from human plasma samples by liquid-liquid extraction technique. The reconstituted samples were chromatographed on a reversed -phase column C18, 50 × 2.1 mm, 5 µm from Waters, by using a 80:20 (v:v) mixture of acetonitrile and 0.1% formic acid in water as the mobile phase at a flow rate of 0.5 mL/min. The calibration curve was linear (r 2 > 0.99) over the concentration range of 5.0-1500.0 ng/mL. The results of the intra-and inter-day precision and accuracy studies were within the acceptable limits. A run time of 0.5 min for each sample made it possible to analyze more than 400 plasma samples per day.

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Section: Trideoxy-3-(dimethylamino)-β-d-xylo-]oxy}-1-oxa-6-azacyclopementioning

confidence: 99%

A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma

Younes

Elkady

2014

Eur. J. Chem.

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“…Other analytical techniques also employed for azithromycin determination were voltammetry, 16 amperometry, 17 spectrofluorimetry, 18 chemiluminescence 19 and spectrophotometry. [20][21][22][23][24] Although the low cost and simplicity of molecular absorption spectrophotometry only few papers related the use of this technique for azithromycin determination.…”

Section: Introductionmentioning

confidence: 99%

Novel spectrophotometric method for the determination of azithromycin in pharmaceutical formulations based on its charge transfer reaction with quinalizarin

Paula¹,

Almeida²,

Cassella³

2010

J. Braz. Chem. Soc.

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Neste trabalho é proposto um novo método para a determinação espectrofotométrica simples e rápida de azitromicina em formulações farmacêuticas. O método está baseado na reação de transferência de carga entre a azitromicina e a quinalizarina em meio de metanol. O efeito de diversas variáveis foi avaliado com o intuito de alcançar máxima sensibilidade analítica. A reação de transferência de carga foi caracterizada, sendo determinadas a estequiometria da reação, a absortividade molar aparente e a constante de associação. Melhores resultados foram obtidos em meio de metanol e com uma concentração de quinalizarina de 50 mg L -1 . Nestas condições, o ânion radicalar (produto da reação de transferência de carga) foi formado imediatamente após a mistura dos reagentes, apresentando máxima absorção em 564 nm. O método apresentou um limite de detecção de 0,35 mg L -1 e um limite de quantificação de 1,2 mg L -1 , sendo aplicado com sucesso na determinação de azitromicina em três amostras de medicamentos comerciais contendo azitromicina. This paper proposes a new method for simple and fast spectrophotometric determination of azithromycin in pharmaceutical formulations. The method is based on the charge transfer reaction between the azithromycin and quinalizarin in methanol medium. In order to achieve maximum sensitivity the effect of some chemical variables such as the type of solvent, reagent concentration and reaction time were evaluated. The reaction was characterized in terms of stability of the product formed and its stoichiometry, and the apparent molar absorptivity and association constant were derived. Best conditions for the analytical determination of azithromycin were observed in methanol medium with a quinalizarin concentration of 50 mg L -1 . At these conditions, the radical anion (absorbing specie) was formed in the medium immediately after mixing of the reagents and showed maximum absorption at 564 nm. The method presented a limit of detection of 0.35 mg L -1 and a limit of quantification of 1.2 mg L -1 . It was successfully applied in the determination of azithromycin in three commercial pharmaceutical formulations of azithromycin and no matrix interferences were observed.

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“…1,2 It is used in respiratory tract infections, like pharyngitis, pneumonia, chronic bronchitis, bronchopneumonia, skin and soft tissue infections and some sexually transmitted diseases. [2][3][4][5] Several methods have been described in the literature for the determination of AZ in pharmaceutical formulations and biological fluids, including spectrophotometry, 2,6-10 spectrofluorometry, 11 voltammetry, 4,5,12,13 high-performance liquid chromatography using electrochemical, 14,15 amperometric, 16 fluorescence, 17 UV, 18,19 and electrospray ionization mass spectrometry, 20 detection and bioassay. 21,22 Some of these methods display good detection limits and accuracy.…”

Section: Introductionmentioning

confidence: 99%

“…21,22 Some of these methods display good detection limits and accuracy. 5,[11][12][13][14][15][16][17][18]20,22 However, most of them are time-consuming 2,4-9,11 -13,15,17,21,22 and in some cases require expensive equipment. 11,[14][15][16][17][18][19][20] The United States Pharmacopeia 23 proposes a chromatographic method with amperometric detection that needs the use of a specific "Gamma-alumina" column, which is expensive and difficult to obtain commercially.…”

Section: Introductionmentioning

confidence: 99%

Flow-Injection Spectrophotometric Determination of Azithromycin in Pharmaceutical Formulations Using p-Chloranil in the Presence of Hydrogen Peroxide

Rufino

Pezza

2008

ANAL. SCI.

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A flow-injection (FI) spectrophotometric procedure exploiting merging zones is proposed for the determination of azithromycin in pharmaceutical formulations. The method is based on the reaction of azithromycin with tetrachloro-pbenzoquinone (p-chloranil) accelerated by hydrogen peroxide and conducted in a methanol medium, producing a purplered color compound (lmax = 540 nm). The FI system and the experimental conditions were optimized using a multivariate method. Beer's law is obeyed in a concentration range of 50 -1600 mg mL -1 with an excellent correlation coefficient (r = 0.9998). The detection limit and the quantification limit were 6.6 and 22.1 mg mL -1 , respectively. No interference was observed from the common excipients, and the recoveries were within 98.6 to 100.4%. The procedure was applied to the determination of azithromycin in pharmaceuticals with a high sampling rate (65 samples h -1 ). The results obtained by the proposed method were in good agreement with those obtained by the comparative method at 95% confidence level.

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Spectrofluorimetric analysis of certain macrolide antibiotics in bulk and pharmaceutical formulations

Cited by 58 publications

References 26 publications

A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma

A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma

Novel spectrophotometric method for the determination of azithromycin in pharmaceutical formulations based on its charge transfer reaction with quinalizarin

Flow-Injection Spectrophotometric Determination of Azithromycin in Pharmaceutical Formulations Using p-Chloranil in the Presence of Hydrogen Peroxide

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