A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma

Younes, Kareem M.; Elkady, Ehab F.

doi:10.5155/eurjchem.5.2.241-246.1000

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Spectrometric methods include UV-VIS spectrophotometric [9] and spectrofluorimetric methods [10], NMR spectroscopy [11] and electrospray tandem mass spectrometry [12]. Examples of HPLC and gas chromatography comprise: liquid chromatography-electrospray-mass spectrometry (MS) [13], ultra-performance liquid chromatography with tandem MS [14][15][16], HPLC coupled with electrochemical detection [17], HPLC with UV detector [18], rapid resolution reversed-phase HPLC with diode array detector [19] and gas chromatography coupled with MS [20].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode

Lović¹,

Trišović

Antanasijević

et al. 2016

Journal of Electroanalytical Chemistry

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Nonmedical use of sildenafil citrate (SC) requires new methods for drug determination in human serum and in tablets. SC as standard and in Sildena ® tablets is determined by square wave voltammetry (SWV) in 0.1 M H 2 SO 4 (harmful for human serum) at gold electrode in a range: (110 -3 , 110 -2 , 0.1, 0.5, 1) μM and on cysteine (Cys) modified gold electrode (Au/Cys) in a range: (110 -3 , 110 -2 , 0.05, 0.1) μM. The presence of Cys causes two times larger peak currents and shifting of the incipient potential of the SC oxidation to 0.1 V in negative direction. A safe procedure for SC determination as standard and in Sildena ® tablets spiked with human serum is developed by SWV at gold electrode in 0.05 M NaHCO 3 (suitable for human liquids) in a range: (110 -3 , 110 -2 , 0.1, 0.2, 0.3) μM. Microscopic characterization of the surfaces morphology was also performed. The SC concentrations were checked by High Performance Liquid Chromatography-Ultraviolet spectroscopy (HPLC-UV) showing that electrochemical method is more sensitive and can be used for the measurements of very low concentrations of the analyte.

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Section: Introductionmentioning

confidence: 99%

Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode

Lović¹,

Trišović

Antanasijević

et al. 2016

Journal of Electroanalytical Chemistry

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“…In a previous study, LLOQ was obtained at 0.1 ng/ml with a solid phase extraction method using UPLC-MS/MS, and 1.66 ng/ml of Cmax lercanidipine was obtained from healthy subjects [5]. The objective of this research is to obtain an optimum and validated method for analyzing lercanidipine in plasma using Ultra Performance Liquid Chromatography of Tandem Mass Spectrometry (UPLC-MS/MS).…”

Section: Introductionmentioning

confidence: 99%

Method Development and Validation of Lercanidipine in Human Plasma by Liquid Chromatography Tandem-Mass Spectrometry

2018

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Objective: To obtain an optimum and validated method for analyzing lercanidipine in plasma using Ultra Performance Liquid Chromatography of Tandem Mass Spectrometry (UPLC-MS/MS). Methods:The separation was carried out using 1.7μm (2.1 x 100 mm) Waters Acquity TM UPLC C18 column, a mobile phase of the 0.1% formic acidmethanol mixture (20:80 v/v) with isocratic elution, 30 °C column temperature, 0.2 ml/min flow rate and amlodipine as an internal standard. Mass detection was performed with a positive XBL TQD type Electrospray Ionization (ESI) in Multiple Reaction Monitoring modes. Lercanidipine was detected at m/z value of 612.11>280.27 and amlodipine was detected at m/z value 409.1>238.15. The optimum sample preparation method was a liquid-liquid extraction using 5 ml of n-hexane-ethyl acetate (50:50 v/v), vortex mixed for 3 min, centrifuged at 4000 rpm for 20 min, evaporated with nitrogen at 50 °C for 30 min, and the residue was reconstituted with 100 μl of mobile phase. Results:The method was linear in the range of 0.025-10 ng/ml with r ≥ 0.9986. Accuracy and precis ion within-run and between-run met the requirements with %diff and %CV, not exceeding ± 15% and not more than ± 20% for Lower Limit of Quantification (LLOQ) concentration. Conclusion:It was concluded that the developed method met the requirements of selectivity, carry over, stability, the integrity of dilution, and matrix effects under the Guideline on Bioanalytical Method Validation by the European Medicines Agency in 2011.

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A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma

Cited by 2 publications

References 21 publications

Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode

Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode

Method Development and Validation of Lercanidipine in Human Plasma by Liquid Chromatography Tandem-Mass Spectrometry

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