Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens

Mazaheri, A.; Prusas, C.; Voß, Matthias; Heß, Michael

doi:10.1080/03079459808419335

Cited by 124 publications

(85 citation statements)

References 19 publications

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“…However, the presence of FAdV-2 and FAdV-5 serotypes has not been reported previously. Earlier, only serotypes FAdV-4 and -8 were considered to be involved in cases of HPS and IBH, respectively [11]. Identification of serotypes other than FAdV-4 and FAdV-8 in this study suggests their possible role in causation of the disease.…”

Section: Sequence Analysismentioning

confidence: 83%

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Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens

et al. 2013

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The present study was carried out for detection and molecular characterization of fowl adenoviruses (FAdVs) associated with hydropericardium syndrome or inclusion body hepatitis in commercial broiler chickens. The FAdVs were detected in liver samples from 33 commercial broiler chicken flocks by polymerase chain reaction (PCR) using hexon gene specific primers. The restriction enzyme analysis using StyI, BsiWI, MluI, AspI, BglI and ScaI enzymes of all the 33 FAdV-positive samples revealed FAdV-4 in 10 samples, FAdV-8 in five samples, FAdV-2 and FAdV-12 in two samples each, and FAdV-5 and FAdV-6 in one sample each. Twelve samples revealed the digestion pattern for more than one serotypes with FAdV-8 and FAdV-5, FAdV-8 and FAdV-7, FAdV-8 and FAdV-6, FAdV-8 and FAdV-12 being the predominant combinations indicating mixed infection. The serotypes FAdV-2 and FAdV-5 have not been detected previously in the country. The purified PCR products of FAdVs of four samples (HR 1, HR 2, HR 3 and HR 4) were cloned and sequenced. Phylogenetic analysis revealed that FAdVs of all four samples clustered in separate groups consistent with the REA pattern. In conclusion, this study reveals the presence of FAdV-2, FAdV-4, in broiler chickens affected with hydropericardium syndrome or inclusion body hepatitis in India.

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Section: Sequence Analysismentioning

confidence: 83%

“…All 12 serotypes have been incriminated in IBH [2], whereas serotype 4 has been implicated in HPS [9,11].…”

Section: Introductionmentioning

confidence: 99%

Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens

et al. 2013

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“…The ® rst isolation of an adenovirus from ® eld cases was reported by Voû (1989). Recently, we described the successful reproduction of the disease in different aged speci® c pathogen free (SPF) chicks using fowl adenovirus (FAV) serotype 4 strains isolated from Pakistan and Ecuador (Mazaheri et al, 1998). In these investigations, genomic differences were found between the isolates from Pakistan and Ecuador.…”

Section: Introductionmentioning

confidence: 98%

Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium

1999

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Serology, restriction enzyme analysis and polymerase chain reactions were used to classify a total of 12 fowl adenoviruses (FAV) isolated from clinical cases of infectious hydropericardium from ® eld outbreaks in seven countries in Asia and America. All isolates belonged to FAV serotype 4. Two isolates were contaminated with avian adeno-associated virus and one of them also contained FAV1. Minor differences were observed in the BamHI restriction pro® les. More variability was seen with SmaI, BglII and PstI restriction pro® les. However, more than 80% of the fragments were identical in size in the ® ve different PstI pro® les, indicating the close genomic relationship between the isolates. Polymerase chain reaction assays supported the classi® cation of the isolates as FAV4 strains. All isolates could be detected using H1/H2 or H3/H4 primer pairs. Restriction enzyme analysis of the H1/H2 polymerase chain reaction (PCR) products allowed no differentiation between the isolates, whereas the three isolates from India and Pakistan could be separated from all others after HpaII digestion of the H3/H4 PCR products. Although strain variation was demonstrated, it could be shown that all adenoviruses isolated from various ® eld cases of infectious hydropericardium (Angara Disease) in several countries belong to fowl adenovirus serotype 4.

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“…After 24 h incubation at 378C with 5% CO 2 , inoculated monolayers were washed with PBS and then fixed with acetone. To each cover slip, 100 ml specific antiserum was added*which was prepared in our institute in specific pathogen free chickens according to the method described (Mazaheri et al, 1998) using FAdV-1 (CELO virus) antigen*and it was directed against group I avian adenoviruses. The cover slips were incubated for 60 min at room temperature and then washed three times with PBS.…”

Section: Methodsmentioning

confidence: 99%

Adenovirus of psittacine birds: investigations on isolation and development of a real-time polymerase chain reaction for specific detection

et al. 2007

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Liver samples of psittacine birds with a histological suspicion of an adenovirus infection, confirmed by electron microscopy examination, were subjected to virus isolation attempts using a heterologous cell culture system and a homologous cell culture system in the form of chicken embryo liver cells and psittacine embryo fibroblasts, respectively. Whereas isolation in chicken embryo liver cells failed, virus was isolated successfully in the psittacine embryo fibroblasts cell culture system. Molecular investigations identified the virus as a specific psittacine adenovirus (PsAdV). Additionally, on the basis of the hexon gene sequence data obtained, a real-time polymerase chain reaction (PCR) for specific detection of PsAdV was developed. To ensure an exclusive hybridization with PsAdV, selected primers were located within the variable L1 region of the hexon gene. Furthermore, the specificity of the real-time PCR was confirmed by investigation of a panel of different avian adenoviruses and unrelated DNA viruses. Using this PCR, the threshold cycle values obtained support the propagation of PsAdV in the homologous cell culture system in comparison with the chicken cell culture system. Moreover, the developed PCR represents a reliable method for specific and sensitive detection of PsAdV in clinical samples.

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Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens

Cited by 124 publications

References 19 publications

Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens

Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens

Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium

Adenovirus of psittacine birds: investigations on isolation and development of a real-time polymerase chain reaction for specific detection

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