Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium

Heß, Michael; Raue, R.; Prusas, C.

doi:10.1080/03079459994443

Cited by 81 publications

(46 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FAdV-4 is one of the newly emergent serotypes in many regions worldwide. It was reported in Angora Goth close to Karachi in Pakistan for the first time (4). Clinically, FAdV-4 causes several syndromes in the affected chicken populations such as inclusion body hepatitis and hepatitis-hydropericardium syndrome (5,6).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Hemida¹,

Al-Hammadi²

2017

Turk J Vet Anim Sci

View full text Add to dashboard Cite

IntroductionFowl adenoviruses are a diverse group of viruses affecting chickens. They belong to the genus Aviadenovirus in the family Adenoviridae (1). Currently, there are 12 known serotypes of fowl adenoviruses (2). Fowl adenovirus serotype 4 (FAdV-4) belongs to group I and is classified as a member of group C fowl adenoviruses according to restriction fragment length polymorphism (RFLP) (3). FAdV-4 is one of the newly emergent serotypes in many regions worldwide. It was reported in Angora Goth close to Karachi in Pakistan for the first time (4). Clinically, FAdV-4 causes several syndromes in the affected chicken populations such as inclusion body hepatitis and hepatitis-hydropericardium syndrome (5,6). The affected chickens show depression, ruffled feathers, and resting on the ground with their chest close to death (5). Necropsy examination reveals several changes in the liver, such as mottling and being large and pale. Sometimes ecchymotic hemorrhages and multifocal necrotic areas in the parenchyma of the liver are found (7). The clinical picture of FAdV-4-induced hydropericardium syndrome is characterized by the accumulation of jelly-like amber viscous fluid in the pericardial cavities of the affected birds. The infected hearts appear large, flappy, and rounded (7). It is not an easy task to diagnose FAdVs due to the existence of a large number of serotypes. This is in addition to the presence of a heterogeneous population of antibodies against different adenoviruses in the sera of apparently healthy birds. However, the combination of polymerase chain reaction (PCR) and RFLP patterns is one of the most recent trends for the identification of FAdV-4 (8). According to the International Committee of Taxonomy and Nomenclature of Viruses (ICTV), FAdVs were classified into 12 serotypes. Further classification based on RFLP digestion patterns classified them into five groups (A-E) (8,9). Several outbreaks were reported in many Asian countries such as Iraq, India, Japan, and many other countries (10-12). Despite the detection of FAdV-4 in Kuwait (10), nothing is known about its prevalence in the other Gulf countries, especially Saudi Arabia.Several breeder farms reported high morbidity and mortality rates in chickens around 15 weeks old. Birds in these farms showed typical hydropericardium syndrome. Necropsy examination showed that the affected hearts were Abstract: Fowl adenovirus serotype 4 (FAdV-4) is a new emerging viral disease of chickens worldwide. It causes inclusion body hepatitis and hepatitis-hydropericardium syndrome. Little is known about its prevalence in the Middle East. Here we report the prevalence of FAdV-4 in five chicken farms in eastern Saudi Arabia. High mortality rates were reported from birds from those five farms at 15 weeks of age. Gross examination revealed typical hydropericardium syndrome and accumulation of jelly-like materials in the pericardial cavities. We isolated FAdV-4 by using embryonated chicken egg inoculation. The inoculated embryos showed dwarfing, deformities, hemorrhage, an...

show abstract

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Hemida¹,

Al-Hammadi²

2017

Turk J Vet Anim Sci

View full text Add to dashboard Cite

show abstract

“…The gene encoding the hexon protein has been the focus of attention. Hexon gene-based PCRs have been used to confirm the presence of FAdV4 in field outbreaks of hydropericardium, followed by restriction fragment analysis to compare the isolates (Hess et al, 1999). Meulemans et al (2001b) compared the hexon proteins of three FAdV serotypes and designed two primers corresponding to sequences in conserved parts of the two pedestal regions adjacent to the L1 loop region of the hexon protein.…”

Section: Avian Adenovirusesmentioning

confidence: 99%

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Cavanagh¹

2001

Avian Pathology

View full text Add to dashboard Cite

Polymerase chain reaction (PCR)-based approaches to the detection, differentiation and characterization of avian pathogens continue to be developed and refined. The PCRs, or reverse transcriptasePCRs, may be general, designed to detect all or most variants of a pathogen, or to be serotype, genotype or pathotype specific. Progress is being made with respect to making nucleic acid approaches more suitable for use in diagnostic laboratories. Robotic workstations are now available for extraction of nucleic acid from many samples in a short time, for routine diagnosis. Following general PCR, the DNA products are commonly analyzed by restriction endonuclease mapping (restriction fragment length polymorphism), using a small number of restriction endonucleases, based on a large body of sequence data. Increasingly, however, nucleotide sequencing is being used to analyze the DNA product, in part due to the expanding use of non-radioactive sequencing methods that are safe and enable high throughout. In this review, I highlight some recent developments with many avian viruses: Newcastle disease virus; circoviruses in canary and pigeon; infectious bursal disease virus (Gumboro disease virus); avian adenoviruses, including Angara disease/infectious hydropericardium virus, haemorrhagic enteritis virus of turkeys, and egg drop syndrome virus; avian herpesviruses, including infectious laryngotracheitis virus, duck plague virus, psittacine herpesvirus (Pacheco's parrot disease virus), Marek's disease virus and herpesvirus of turkeys; avian leukosis virus (associated with lymphoid leukosis or myeloid leukosis, and egg transmission); avian pneumoviruses (turkey rhinotracheitis virus); avian coronaviruses, including infectious bronchitis virus, turkey coronavirus and pheasant coronavirus; astrovirus, in the context of poult enteritis and mortality syndrome, and avian nephritis virus; and avian encephalomyelitis virus, a picornavirus related to hepatitis A virus.

show abstract

“…Infections with adenovirus (Gassmann et al, 1981;Ramis et al, 1994;Capua et al, 1995;Weissenbö ck & Fuchs, 1995;Soike et al, 1998;Hess et al, 2000) or adenovirus-like particles (Scott et al, 1986;Pass, 1987;Mori et al, 1989;Gomezvillamandos et al, 1995;Mackie et al, 2003) have been also described in a variety of psittacine birds. Most infections were associated with necrotizing hepatitis and basophilic inclusion bodies in hepatocytes caused by different serotypes of FAdV.…”

Section: Introductionmentioning

confidence: 99%

Adenovirus of psittacine birds: investigations on isolation and development of a real-time polymerase chain reaction for specific detection

et al. 2007

Self Cite

View full text Add to dashboard Cite

Liver samples of psittacine birds with a histological suspicion of an adenovirus infection, confirmed by electron microscopy examination, were subjected to virus isolation attempts using a heterologous cell culture system and a homologous cell culture system in the form of chicken embryo liver cells and psittacine embryo fibroblasts, respectively. Whereas isolation in chicken embryo liver cells failed, virus was isolated successfully in the psittacine embryo fibroblasts cell culture system. Molecular investigations identified the virus as a specific psittacine adenovirus (PsAdV). Additionally, on the basis of the hexon gene sequence data obtained, a real-time polymerase chain reaction (PCR) for specific detection of PsAdV was developed. To ensure an exclusive hybridization with PsAdV, selected primers were located within the variable L1 region of the hexon gene. Furthermore, the specificity of the real-time PCR was confirmed by investigation of a panel of different avian adenoviruses and unrelated DNA viruses. Using this PCR, the threshold cycle values obtained support the propagation of PsAdV in the homologous cell culture system in comparison with the chicken cell culture system. Moreover, the developed PCR represents a reliable method for specific and sensitive detection of PsAdV in clinical samples.

show abstract

Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium

Cited by 81 publications

References 13 publications

Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Adenovirus of psittacine birds: investigations on isolation and development of a real-time polymerase chain reaction for specific detection

Contact Info

Product

Resources

About