Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens

Abstract

The present study was carried out for detection and molecular characterization of fowl adenoviruses (FAdVs) associated with hydropericardium syndrome or inclusion body hepatitis in commercial broiler chickens. The FAdVs were detected in liver samples from 33 commercial broiler chicken flocks by polymerase chain reaction (PCR) using hexon gene specific primers. The restriction enzyme analysis using StyI, BsiWI, MluI, AspI, BglI and ScaI enzymes of all the 33 FAdV-positive samples revealed FAdV-4 in 10 samples, FAdV-8 in five samples, FAdV-2 and FAdV-12 in two samples each, and FAdV-5 and FAdV-6 in one sample each. Twelve samples revealed the digestion pattern for more than one serotypes with FAdV-8 and FAdV-5, FAdV-8 and FAdV-7, FAdV-8 and FAdV-6, FAdV-8 and FAdV-12 being the predominant combinations indicating mixed infection. The serotypes FAdV-2 and FAdV-5 have not been detected previously in the country. The purified PCR products of FAdVs of four samples (HR 1, HR 2, HR 3 and HR 4) were cloned and sequenced. Phylogenetic analysis revealed that FAdVs of all four samples clustered in separate groups consistent with the REA pattern. In conclusion, this study reveals the presence of FAdV-2, FAdV-4, in broiler chickens affected with hydropericardium syndrome or inclusion body hepatitis in India.

Epidemiological investigation of outbreaks of fowl adenovirus infections in commercial chickens in China

“…Recently, IBH and HPS cases have been reported and confirmed in Asia and Europe (Asthana, Chandra, & Kumar, ; Choi et al., ; Mittal, Jindal, Tiwari, & Khokhar, ; Ojkic et al., ; Shahzad et al., ), and FAdV strains have been isolated and serologically typed. The genome sequences of these viruses have also been analysed phylogenetically (Kaján, Kecskeméti, Harrach, & BenkÅ, ; Tae‐Hyun et al., ; Toro & Hess, ).…”

Molecular epidemiology of hydropericardium syndrome outbreak-associated serotype 4 fowl adenovirus isolates in central China

“…However, since July 2015, outbreaks of HPS occurred with sudden high mortality rates in broilers in many small and middle chicken forms in central China, even the duck farms, leading to tremendous economic losses. Recent years, the frequency of HPS have also been increasing in many countries, such as India (2014) [18], Canada (2011) [19], Hungary (2013) [20], Korea (2012) [21], Japan (2012) [22], and Poland (2016) [10]. The ON1 and JSJ13 strains in local regions in China didn’t lead to severe clinical symptoms.…”

Identification of four serotypes of fowl adenovirus in clinically affected commercial poultry co‐infected with chicken infectious anaemia virus in Trinidad and Tobago

“…A neighbour joining tree (Figure 1 identity for the four strains that aligned with FAdV-11. and high rates of mortality in the absence of co-infecting viruses (Gomis et al, 2006;Mittal et al, 2014). Virulent strains belonging mainly to serotypes FAdV-8a, FAdV-8b and FAdV-11 were implicated in causing severe outbreaks of IBH in several countries including Canada and Australia (Dar et al, 2012;Gomis et al, 2006;Steer et al, 2015;Steer-Cope, 2015).…”