Some problems of radioimmunoassay control

Závada, M; Safarcík, K; Topolčan, Ondřej

doi:10.1007/bf02519728

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…However, problems associated with this technique are well known. They include cross reactivity with similar analytes, standardisation issues between labs and sensitivity issues (7). It is now recognised that performance of some IA techniques may be suboptimal, this recently prompted a recommendation from the editors of Journal of Clinical Endocrinology and Metabolism to avoid using such assays and instead use MS for the measurement of sex steroids (8).…”

Section: -Franz Kafkamentioning

confidence: 99%

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Taylor¹,

Keevil²,

Huhtaniemi³

2015

European Journal of Endocrinology

249

157

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SummaryThe recent onslaught of mass spectrometry (MS) to measurements of steroid hormones, including demands that they should be the only acceptable method, has confused clinicians and scientists who have relied for more than 40 years on a variety of immunoassay (IA) methods in steroid hormone measurements. There is little doubt that MS methods with their superior specificity will be the future method of choice in many clinical and research applications of steroid hormone measurement. However, the majority of steroid measurements are currently, and will continue to be, carried out using various types of IAs for several reasons, including their technical ease, cost and availability of commercial reagents. Speedy replacement of all IAs with MS is an unrealistic and unnecessary goal, because the availability of MS measurements is limited by cost, need of expensive equipment, technical demands and lack of commercial applications. Furthermore, IAs have multiple well-known advantages that vindicate their continuing use. The purpose of this article is to elucidate the advantages and limitations of the MS and IA techniques from two angles, i.e. promotion of MS and defence of IA. The purpose of the text is to give the reader an unbiased view about the current state and future trends of steroid analysis and to help him/her choose the correct assay method to serve his/her diagnostic and research needs.

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Section: -Franz Kafkamentioning

confidence: 99%

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Taylor¹,

Keevil²,

Huhtaniemi³

2015

European Journal of Endocrinology

249

157

View full text Add to dashboard Cite

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“…The specificity of this technique is well documented, and the possibility of measuring multiple analytes simultaneously is an advantage [ 26 , 27 ]. Immunoassays such as ELISA are noted for their high-throughput-ness and their high sensitivity; however they are known to include unspecific binding of molecules with similar structures [ 28 ]. When performing the ELISA method, it is not possible to discern what percentage of the detected corticosteroid represents cortisol versus non-specifically bound cortisone, whereas the LC-MS method is capable of measuring concentrations of each independently because of its specificity in identifying these differently functioning molecules [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Diurnal Hypothalamic-Pituitary-Adrenal Axis Measures and Inflammatory Marker Correlates in Major Depressive Disorder

Doolin

Farrell

Tozzi

et al. 2017

IJMS

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Dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis and inflammatory systems is a consistent finding in patients with Major Depressive Disorder (MDD). Cortisol is often assessed by measurement of the cortisol awakening response (CAR) and/or diurnal cortisol levels. Some methods of cortisol measurement overestimate cortisol concentration due to detection of other glucocorticoids including the relatively inert cortisone, therefore this study aimed to assess the presence of both cortisol and cortisone, and the cortisol-cortisone catalyzing enzyme 11β-hydroxysteroiddehydrogenase type 1 (11β-HSD1), in depressed patients and controls. Because the HPA axis is known to regulate the body’s immune system, relationships between measures of cytokines and cortisol were also assessed. Saliva samples were collected from 57 MDD patients and 40 healthy controls at five post-wakening time points (0, +30, +60, +720 and +750 min). Glucocorticoid concentrations were measured by liquid chromatography mass spectrometry. Whole blood mRNA expression of several inflammatory markers was measured by quantitative polymerase chain reaction. This study replicated the common finding of elevated morning cortisol and reduced CAR reactivity in MDD and found no differences in cortisone or 11β-HSD1 mRNA measures. There was a negative association between interleukin 1-β (IL-1β) mRNA and morning cortisol reactivity within the depressed group, indicating that dysregulation of the HPA axis and immune system may be interconnected.

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“…IAs were developed in parallel to MS and were generally used for analysis in clinical works due to their sensitivity, simplicity, and cost. Nevertheless, this method has several disadvantages, such as cross-reactivity with similar analytes, standardization of steroid hormone measurements between laboratories, and sensitivity issues [9]. In recent years, LC-MS/MS has been usually chosen for estimating steroid hormones in the clinical research labs because of shorter sample preparation than for GC-MS and increased specificity and sensitivity in comparison with IAs [2].…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Simultaneous Measurement of Steroid Hormone in Human Serum by Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry

Khánh¹,

Hải²,

Thuy³

et al. 2022

MPS

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A high sensitive simultaneous determination method of nine steroid hormones including Estrone (E1), estradiol (E2), testosterone (T), dihydrotestosterone (DHT), 17-hydroxyprogesterone (17OH-P4), androstenedione (AN), progesterone (P4), dehydroepiandrosterone (DHEA), and cortisol by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was established in a positive mode using recently developed picolinyl derivatization. Steroid hormones were derived with picolinic acid and 2-methyl-6-nitrobenzoic anhydride, then purified by solid-phase extraction with InterSep SI cartridge. The LC-ESI-MS/MS method enhanced the specificity and sensitivity for E1, E2, T, DHT, DHEA, and cortisol. The method validation indicated that the limits of quantification for E1, E2, T, DHT, 17OH-P4, AN, P4, DHEA, and cortisol were 1, 1, 1, 2, 2, 2, 2, 20, and 100 pg/tube, respectively with acceptable accuracy and precision within ± 15%. The present method was applied to the measurement of nine steroid hormones in children’s serums with high reliability and reproducibility. Keywords: LC-ESI-MS/MS, steroid hormone, picolinyl derivatization, human serum, solid-phase extraction. References [1] A. R. Vasconcelos, J. V. C. Costa, C .H. Mazucanti, C. Scavone, E. M. Kawamoto, The Role of Steroid Hormones in the Modulation of Neuroinflammation by Dietary Interventions, Front Endocrinol (Lausanne), Vol. 7, 2016, pp. 1-14, https://doi.org/10.3389/fendo.2016.00009.[2] D. French, Advances in Bioanalytical Techniques to Measure Steroid Hormones in Serum, Bioanalysis, Vol. 8, No. 11, 2016, pp. 1203-1219, https://doi.org/10.4155/bio-2015-0025.[3] R. Schoenheimer, D. Rittenberg, Deuterium as an Indicator in the Study of Intermediary Metabolism, Science, Vol. 82, No. 2120, 1935, pp. 156-157, https://doi.org/ 10.1126/science.82.2120.156.[4] P. Eneroth, K. Hellstroem, R. Ryhage, Idenfication and Quatification of Neutral Fecal Steroids by Gas-liquid Chromatography and Mass Spectrometry: Studies of Human Excretion during Two Dietary Regimens, J Lipid Res, Vol. 5, 1964, pp. 245-262.[5] R. Rhyage, Use of a Mass Spectrometer as a Detector and Analyzer for Effluent Emerging from High Temperature Gas Liquid Chromatography Columns, Vol. 36, No.4, 1964, pp. 759-764, https://doi.org/10.1021/ac60210a019 36. [6] G. E. Abraham, Solid-phase Radioimmunoassay of Estradiol-17 Beta, J Clin Endocrinol Metab, Vol. 29, No. 6, 1969, pp. 866-870, https://doi.org/10.1210/jcem-29-6-866.[7] M. Zendjabil, Z. Chellouai, O. Abbou, Role of Mass Spectrometry in Steroid Assays, Ann Endocrinol (Paris), Vol. 77, No. 1, 2016, pp. 43-48, https://doi.org/10.1016/j.ando.2016.01.004.[8] C. Shackleton, Clinical Steroid Mass Spectrometry: a 45-year History Culminating in HPLC-MS/MS Becoming an Essential Tool for Patient Diagnosis, J Steroid Biochem Mol Biol, Vol. 121, No. 3-5, 2010, pp. 481-490, https://doi.org/10.1016/j.jsbmb.2010.02.017.[9] M. Závada, K. Šafarčik, O. Topolčan, Some Problems of Radioimmunoassay Control, Journal of Radioanalytical Chemistry, Vol. 46, No. 1, 1978, pp. 57-66.[10] M. Z. Nouri, K. J. Kroll, M. Webb, N. D. Denslow, Quantification of Steroid Hormones in Low Volume Plasma and Tissue Homogenates of Fish using LC-MS/MS, Gen Comp Endocrinol, Vol. 296, pp. 1-15, https://doi.org/10.1016/j.ygcen.2020.113543.[11] N. Kadian, K. S. R. Raju, M. Rashid, M. Y. Malik, I. Taneja, M. Wahajuddin, Comparative Assessment of Bioanalytical Method Validation Guidelines for Pharmaceutical Industry, J Pharm Biomed Anal, Vol. 126, 2016, pp. 83-97, https://doi.org/10.1016/j.jpba.2016.03.052.[12] Food and Drug Administration, Bioanalytical Method Validation Guidance for Industry, USA, 2018, pp. 1-44.[13] European Medicines Agency, Guideline on Bioanalytical Method Validation, Italy, 2012, pp. 1-23.[14] T. Higashi, S. Ogawa, Chemical Derivatization for Enhancing Sensitivity during LC/ESI-MS/MS Quantification of Steroids in Biological Samples: a Review, J Steroid Biochem Mol Biol, Vol. 162, 2016, pp. 57-69, https://doi.org/10.1016/j.jsbmb.2015.10.003.[15] L. Aksglaede, A. Juul, H. Leffers, N. E. Skakkebaek, A. M. Andersson, The Sensitivity of the Child to Sex Steroids: Possible Impact of Exogenous Estrogens, Hum Reprod Update, Vol. 12, No. 4, 2006, pp. 341-349, https://doi.org/10.1093/humupd/dml018.[16] E. Benyi, L. Sävendahl, The Physiology of Childhood Growth: Hormonal Regulation, Horm Res Paediatr, Vol. 88, No. 1, 2017, pp. 6-14, https://doi.org/10.1159/000471876.[17] J. Cui, Y. Shen, R. Li, Estrogen Synthesis and Signaling Pathways during Aging: From Periphery to Brain, Trends in Molecular Medicine, Vol. 19, No. 3, 2013, pp. 197-209, https://doi.org/10.1016/j.molmed.2012.12.007.[18] G. Jasienska, R. G. Bribiescas, A. S. Furberg, S. Helle, A. N. L. Mora, Human Reproduction and Health: an Evolutionary Perspective, Lancet, Vol. 390, No. 10093, 2017, pp. 510-520, https://doi.org/10.1016/S0140-6736(17)30573-1.[19] A. E. Kulle, F. G. Riepe, D. Melchior, O. Hiort, P. M. Holterhus, A Novel Ultrapressure Liquid Chromatography tandem Mass Spectrometry Method for the Simultaneous Determination of Androstenedione, Testosterone, and Dihydrotestosterone in Pediatric Blood Samples: Age- and Sex-specific Reference Data, J Clin Endocrinol Metab, Vol. 95, No. 5, 2010, pp. 2399-409, https://doi.org/10.1210/jc.2009-1670.[20] A. L. Southren, S. Tochimoto, N. C. Carmody, K. Isurugi, Plasma Production Rates of Testosterone in Normal Adult Men and Women and in Patients with the Syndrome of Feminizing Testes, The Journal of Clinical Endocrinology & Metabolism, Vol. 25, No. 11, 1965, pp. 1441-1450, https://doi.org/10.1210/jcem-25-11-1441.[21] R. S. Swerdloff, R. E. Dudley, S. T. Page, C. Wang, W. A. Salameh, Dihydrotestosterone: Biochemistry, Physiology, and Clinical Implications of Elevated Blood Levels, Endocr Rev, Vol. 38, No. 3, 2017, pp. 220-254, https://doi.org/10.1210/er.2016-1067.[22] J. P. Mulhall, L. W. Trost, R. E. Brannigan,E. G. Kurtz, J. B. Redmon, K. A. Chiles, D. J. Lightner, M. M. Miner, M. H. Murad, C. J. Nelson, E. A. Platz, L. V. Ramanathan, R. W. Lewis, Evaluation and Management of Testosterone Deficiency: AUA Guideline, J Urol, Vol. 200, No. 2, 2018, pp. 423-432, https://doi.org/10.1016/j.juro.2018.03.115.[23] H. Frederiksen, T. H. Johannsen, S. E. Andersen,J. Albrethsen, S. K. Landersoe, J. H. Petersen, A. N. Andersen, E. T. Vestergaard, M. E. Schorring, A. Linneberg, K. M. Main, A. M. Andersson, A. Juul, Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS, J Clin Endocrinol Metab, Vol. 105, No. 3, 2020, pp. 754-68, https://doi.org/10.1210/clinem/dgz196.[24] K. Yamashita, M. Okuyama, R. Nakagawa, S. Honma, F. Satoh, R. Morimoto, S. Ito, M. Takahashi, M. 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show abstract

Some problems of radioimmunoassay control

Cited by 5 publications

References 15 publications

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Diurnal Hypothalamic-Pituitary-Adrenal Axis Measures and Inflammatory Marker Correlates in Major Depressive Disorder

Highly Sensitive Simultaneous Measurement of Steroid Hormone in Human Serum by Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry

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