Some modifications in transplantation of autologus non -cultured melanocytes-keratinocytes suspension in treatment of segmental and focal vitiligo (Egyptian Experience in Alexandria University)

Sobhy, Nagat; Atia, A.E.; El-Ramly, M. S.

doi:10.7241/ourd.20131.01

Cited by 6 publications

(9 citation statements)

References 13 publications

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“…Munish's 4 study revealed excellent color matching in 79% patients. Color match was same as that of the surrounding normally pigmented skin in majority of patients, 90.0%, in a study by Sobhy et al 11 conducted among 20 stable vitiligo patients. In a study done by Huggins et al 12 with 29 treated lesions, good color match was noted in 62% of cases.…”

Section: Jmedsmentioning

confidence: 59%

A Study on Effectiveness of Platelet-poor Plasma as a Media in Autologous Noncultured Epidermal Cell Suspension in the Treatment of Stable Vitiligo

Nagaraju¹,

Abhilasha²

2018

The Journal of Medical Sciences

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Introduction: Replenishing melanocytes selectively in vitiliginous macules by autologous noncultured epidermal cell suspension (NECS) is a promising approach. Limitation of this surgery is its expensive reagents. Platelet-poor (PPP) plasma may replace these expensive reagents.Objective: To evaluate the surgical results when two reagents, namely trypsin inhibitor and Dulbecco's modified Eagle's medium (DMEM), are replaced by patient's PPP as media in NECS.Materials and methods: Descriptive and inferential statistical analysis has been carried out in a clinical study that enrolled 60 stable vitiligo lesions from 60 patients who formed the study group. Patients received melanocytes suspended in their own PPP.Results: Results show that 96.7% repigmentation was achieved in 29 of 30 lesions. Additionally, 3.3% lesions achieved poor repigmentation. Statistical analysis revealed no significant differences with respect to the repigmentation achieved or adverse effects observed.Discussion: Trypsin inhibitor can be replaced by blood plasma as plasma contains alpha 1 antitrypsin, which covalently binds and inactivates action of trypsin. Effective results with blood plasma was possibly due to growth factors in serum which may enhance activity of melanocytes. It also enhances the viscosity of suspension. Conclusion:Platelet-poor plasma can be one of the novel surgical modalities. It is cost-effective and beneficial for our vitiligo patients.

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Section: Jmedsmentioning

confidence: 59%

A Study on Effectiveness of Platelet-poor Plasma as a Media in Autologous Noncultured Epidermal Cell Suspension in the Treatment of Stable Vitiligo

Nagaraju¹,

Abhilasha²

2018

The Journal of Medical Sciences

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“…Sobhy et al [11], also reported earlier repigmentation and better prognosis in age group<15 years than in older patients, probably due to younger cell that grows and multiplies rapidly.…”

Section: Age At Presentation Versus Extent Of Pigmentation At 6 Monthsmentioning

confidence: 91%

“…Verma et al [12], in their study, non-cultured melanocyte technique group included 47.4% (9) females and 52.6% (10) males while Sobhy et al [11] study showed 13 female and 7 male patients.…”

Section: Sexmentioning

confidence: 92%

“…Sobhy et al [11], reported excellent repigmentation 35% test sites, good repigmentation in 55% sites, fair repigmentation in 10% sites and did not report poor repigmentation at any site.…”

Section: Extent Of Pigmentationmentioning

confidence: 93%

“…Sobhy et al [11], study showed the age of 20 patients varied between 13 and 33 years, highest proportion of studied patients was 60% in age group<15 years, and lowest was 40% in age group 15-33 years.…”

Section: Agementioning

confidence: 95%

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A Study of Autologous Epidermal Non-cultured Cell Suspension in Stable Vitiligo Patients

Thejaswi¹,

M²

2019

J Clin Exp Dermatol Res

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Introduction To study the extent of repigmentation after autologous epidermal non-cultured cell suspension in stable vitiligo patients. Patients and Methods A prospective, longitudinal study was conducted to study the extent of repigmentation after autologous epidermal non-cultured cell suspension in 30 stable vitiligo patients Results By subjective assessment, 10% (3) patients showed poor response, 30% (9) showed good response and 60% (18) patients showed excellent response after follow-up at 6 months with good patient satisfaction. By objective assessment 10% (3) patients showed poor response, 13.3% (4) showed fair response and 23.3% (7) patients showed good response, 53.3% (16) patients showed excellent response after follow-up at 6 months. Extent of repigmentation was evaluated as-10% (3) showed poor response, 10% (3) showed fair response and 20% (6) patients showed good response, 60% (18) patients showed excellent response after follow-up at 6 months. Conclusion Autologous non-cultured epidermal cell suspension in stable vitiligo is a safe, effective, simple method, with superior quality of repigmentation.

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Combination of epidermal keratinocyte–melanocyte cells suspension and microneedling: Safe surgical approach in vitiligo

Nilforoushzadeh

Nouri

Alavi

et al. 2022

J of Cosmetic Dermatology

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Introduction Vitiligo is a skin disease that is associated with impaired skin immune systems and pigment degradation in skin melanocyte cells. Despite the significant impact of the disease on the quality of life of patients, treatment of the disease using an effective method such as the transplantation of uncultivated melanocytes was considered by researchers around the world. The goal of this research was to use microneedling to transplant epidermal keratinocyte–melanocyte cells suspension for the treatment of vitiligo patients. Methods In this study, 15 male and female vitiligo in face region patients aged 18–45 years were studied. In this study, melanocyte–keratinocyte cells suspension was sprayed. Patients underwent microneedling treatment after spraying the cells. Before and after transplantation, patients were biometrically examined, and the quantity of pigmentation and changes in the transplanted region were documented. Statistical software was used to examine the results. Results The color difference between the lesion area and normal skin in one, two, and six months after treatment with cell suspension was significantly reduced compared with before treatment (by 48.95%). Moreover, the amount of melanin was significantly increased 6 months after treatment compared with before treatment (129.8 ± 4.16 vs. 195.2 ± 3.54, p = 0.000). A significant decrease in skin brightness in the skin of the lesion area was observed compared with normal skin, 6 months after treatment compared with before treatment (43.7 ± 1.44 vs. 27.9 ± 1.24, p = 0.000). Conclusion Epidermal keratinocyte–melanocyte cells suspension in combination with microneedling could be considered as an effective treatment of vitiligo.

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Some modifications in transplantation of autologus non -cultured melanocytes-keratinocytes suspension in treatment of segmental and focal vitiligo (Egyptian Experience in Alexandria University)

Cited by 6 publications

References 13 publications

A Study on Effectiveness of Platelet-poor Plasma as a Media in Autologous Noncultured Epidermal Cell Suspension in the Treatment of Stable Vitiligo

A Study on Effectiveness of Platelet-poor Plasma as a Media in Autologous Noncultured Epidermal Cell Suspension in the Treatment of Stable Vitiligo

A Study of Autologous Epidermal Non-cultured Cell Suspension in Stable Vitiligo Patients

Combination of epidermal keratinocyte–melanocyte cells suspension and microneedling: Safe surgical approach in vitiligo

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