Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local

Manoj, K.; Akhtar, Nasim; Jaiswal, Vivek

doi:10.1016/j.scienta.2007.02.010

Cited by 66 publications

(47 citation statements)

References 13 publications

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“…Contrary to reports in which somatic embryogenic callus of guava was obtained (Vilchez et al, 2002;Chandra et al, 2004;Rai et al, 2007), the 2.4-D alone did not induce embryogenic calli in the Paluma cultivar. That differentiated response is probably related with the interaction between treatments and the genotype of the cultivar Paluma.…”

Section: Resultscontrasting

confidence: 89%

“…Rai et al (2007) confirmed that lower concentration of sucrose did not lead to somatic embryogenesis induction in guava zygotic embryos. The high concentration of sucrose and other polysaccharides in the medium induces an osmotic effect, creating stress that can lead to embryogenesis induction, and, besides, generates energy to the process (Agarwal et al, 2004).…”

Section: Resultssupporting

confidence: 66%

“…The frequency of embryogenic callus induction in the explants was 7%, similar to the one obtained by Vilchez et al (2002). Rai et al (2007) obtained from 7 to 10% of somatic embryogenesis when zygotic embryos of guava were submitted to constant exposition of 2.4-D.…”

Section: Resultsmentioning

confidence: 99%

“…Besides, the concentration of 2-iP was twice the concentration of 2.4-D. There are other examples of somatic embryogenesis induction in Myrtaceae species when cytokinins and auxins were used (Parra & Amo-Marco, 1998;Stefanello et al 2005). According to Gaj et al (2004), when cytokinin induces somatic embryogenesis, it is almost always combined with an auxin.…”

Section: Resultsmentioning

confidence: 99%

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Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma

Moura

Motoike

2009

Rev. Bras. Frutic.

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The biotechnological techniques may help solve many problems of guava culture, such as the high perishability of fruits. Somatic embryogenesis can generate highly multiplicative cell cultures and with high regenerative potential, serving as basis for genetic transformation. The aim of this work was to obtain somatic embryogenesis of guava (Psidium guajava L.) cv. Paluma. Immature seeds were used, and they were inoculated in MS environment containing 400 mg L-1 of L-glutamine, 100 mg L-1 myo-inositol, 60 g L-1 sucrose, 100 mg L-1 ascorbic acid and supplemented with different types and concentrations of growth regulators. Embryogenic callus appeared after 37 days of culture in an environment containing 1.0 mg L-1 2.4-D + 2.0 mg L-1 2-ip, in 7% of the explants. After 65 days of culture, the treatment containing 0.5 mg L-1 CPA showed 20% of explants with direct embryos, while the treatment with 1 mg L-1 had 14% of explants with direct embryos and 7% of explants with embryogenic callus. In 66.6% of embryos regenerated with 0.5 mg L¹ CPA there was the formation of secondary embryos. The use of IASP and BAP, aiming embryogenesis proliferation, led to an increase in the cellular proliferation, but calli apparently lost their embryogenic potential.

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Section: Resultscontrasting

confidence: 89%

Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma

Moura

Motoike

2009

Rev. Bras. Frutic.

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“…Sucrose is the most conjoint carbohydrate source used in the plant tissue culture and present dominantly in the phloem (Zimmerman and Zeigler, 1975 guava with less time supports our previous report by Chandra et al (2004) in mesocarp tissue of guava. However, at lower concentration (2%, 3%, and 4%) of sucrose not efficiently induce somatic embryogenesis (Rai et al, 2007). High carbohydrate content in culture medium found increased somatic embryogenesis in many plant species (Luo et al, 1996;Rai et.…”

Section: Effect Of Carrier Substrate In Acclimatization Of Plantletsmentioning

confidence: 99%

Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.

Bajpai

Kalim

Chandra

et al. 2016

Braz. arch. biol. technol.

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ISSR marker‐based detection of genomic stability in Cassia occidentalis L. plantlets derived from somatic embryogenesis

Naz

Anis

Alatar

2015

Engineering in Life Sciences

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An efficient in vitro technology has been designed for mass multiplication of Cassia occidentalis (coffee senna) through somatic embryogenesis. Genetic stability of both regenerants and mother plant was evaluated. Embryogenic calli were produced on Murashige and Skoog (MS) medium supplemented with 20.0 μM 2,4‐dichlorophenoxy acetic acid (2,4‐D). Induction of somatic embryos occurred after transfer of calli to medium with reduced concentration of 2,4‐D (10.0 μM) fortified with 1.0 μM abscisic acid (ABA). Subculturing of these embryos onto the maturation medium (1.5 μM 6‐benzyladenine + 1.0 μM ABA + 0.3 μM α‐naphthalene acetic acid) favored progression of the embryos through torpedo, heart‐shaped, and cotyledonary stages; one‐half MS medium was considered the best for conversion of cotyledonary stage embryos to young plantlets. The plantlets were acclimatized to autoclaved soil rite, after which they were transferred to the green house. Among the survived plantlets, 10 plants for each primer were randomly selected for inter‐simple sequence repeat (ISSR) analysis. Of the 10 primers tested, 5 produced reproducible and monomorphic bands, 2 led to minor variation with the appearance of unique bands, and the remaining 3 did not show any banding pattern. The majority of the regenerants had similar characteristics to the donor plant, suggesting genetic homogeneity of in vitro raised plants.

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Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local

Cited by 66 publications

References 13 publications

Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma

Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma

Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.

ISSR marker‐based detection of genomic stability in Cassia occidentalis L. plantlets derived from somatic embryogenesis

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