Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.

Bajpai, Anju; Kalim, Shahina; Chandra, Ramesh; Kamle, Madhu

doi:10.1590/1678-4324-2016150170

Cited by 12 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Titon, Xavier, Otoni, and Motoike (2007), picloram is used for the induction of calli or the maintenance of cultures in suspensions, as well as for inducing the formation of embryogenic calli. Most studies have indicated that calli are achieved primarily through the use of 2,4-D (Bajpai, Kalim, Chandra, & Kamle, 2016;Fehér, 2015;Rocha et al, 2016;Winkelmann et al, 2015), including in the induction of calogenesis in Campomanesia rufa leaf explants (Sant'Ana, Paiva, Reis, Silva, & Silva, 2018), which was not found in the present study. Satisfactory results have been achieved using picloram for embryogenic callus induction in Eucalyptus grandis x E. urophylla (Moura et al, 2017) and Acca sellowiana (Cangahuala-Inocente, Caprestano, & Pierre, 2007), both from the same family, Myrtaceae, as gabiroba.…”

Section: Discussioncontrasting

confidence: 70%

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Rossato

Schumacher

Netto

et al. 2019

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA, NAA or picloram. Cytochemical and SEM analyses revealed cellular characteristics of the formation of meristematic centers that indicated 4.14 μM of picloram to be the best treatment for induction of embryogenic calli, and demonstrating their embryogenic potential. The treatment was used to establish a callus growth curve, from which it was inferred that calli should be transferred to new culture media on the 28th day to maintain cell viability

show abstract

Section: Discussioncontrasting

confidence: 70%

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Rossato

Schumacher

Netto

et al. 2019

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…During the first phase of embryo maturation, the presence of 30 g L -1 PEG-4000 in the culture medium favored the maturation of SE until the torpedo stage. Similarly PEG-3350 (50 g L -1 ) stimulated the development and maturation of SEs of Myrciaria aureana, another Myrtaceae species (Motoike et al 2007). Bajpai et al (2016 used the same concentration for SE maturation of Psidium guajava and obtained 74.38% of cotyledonary embryos.…”

Section: Discussionmentioning

confidence: 99%

Embryogenic cultures and somatic embryos development from mature seeds of jabuticaba (Plinia cauliflora (Mart.) Kausel)

Oliveira

Sant’Anna-Santos

Fraga

et al. 2022

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Plinia cauliflora is an important Brazilian species that produces highly appreciated fruits, with a great potential of commercialization. However, the high cost of seedlings is a bottleneck for the expansion of commercial orchards. The present study aimed to investigate somatic embryogenesis as a propagation method for P. cauliflora using seeds as explants. To induce embryogenic mass (EM) and somatic embryo (SE) development we evaluated the supplementation of culture medium with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), combined or not with activated charcoal (AC). For the embryo maturation, we investigated the effects of AC, polyethylene glycol (PEG), Gelzan ® , 6-benzylaminopurine and gibberellin supplementation. For the EM induction, the best results were obtained in MS culture medium supplemented with 300 μM 2,4-D and 1 g L -1 AC. During the first maturation phase, the supplementation of 30 g L -1 PEG improved the somatic embryo formation at the torpedo and cotyledonary stages, whereas the maturation treatments did not result in the conversion of the embryos into plantlets. The anatomical analysis showed that the 2,4-D presence for 60 days may have been deleterious for embryonic development. These results represent the first report of P. cauliflora somatic embryogenesis and its feasibility for mass propagation.

show abstract

“…The authors found that higher concentrations (5 μM) of 2,4-D favored tissue differentiation and somatic embryo formation. It is observed in the literature that the use of 2,4 -D individually has greater success (Bajpai et al 2016, Kamle andBaek 2017). Luis and Scherwinski-Pereira (2014) efficiently induced embryogenic callus formation in Acrocomia aculeate by using low concentration of 2,4-D (1.5 mg L -1 ).…”

Section: Mcr Araújo Et Almentioning

confidence: 99%

Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Araújo

Chagas

Vendrame

et al. 2021

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g -1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L -1 ) and BAP (0, 0.25, 0.5 and 1 mg L -1 ). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L -1 , 2,4-D and 1 mg L -1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.

show abstract

Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.

Abstract: A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites

Cited by 12 publications

References 45 publications

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Embryogenic cultures and somatic embryos development from mature seeds of jabuticaba (Plinia cauliflora (Mart.) Kausel)

Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Contact Info

Product

Resources

About