ISSR marker‐based detection of genomic stability in Cassia occidentalis L. plantlets derived from somatic embryogenesis

Abstract

An efficient in vitro technology has been designed for mass multiplication of Cassia occidentalis (coffee senna) through somatic embryogenesis. Genetic stability of both regenerants and mother plant was evaluated. Embryogenic calli were produced on Murashige and Skoog (MS) medium supplemented with 20.0 μM 2,4‐dichlorophenoxy acetic acid (2,4‐D). Induction of somatic embryos occurred after transfer of calli to medium with reduced concentration of 2,4‐D (10.0 μM) fortified with 1.0 μM abscisic acid (ABA). Subculturing of these embryos onto the maturation medium (1.5 μM 6‐benzyladenine + 1.0 μM ABA + 0.3 μM α‐naphthalene acetic acid) favored progression of the embryos through torpedo, heart‐shaped, and cotyledonary stages; one‐half MS medium was considered the best for conversion of cotyledonary stage embryos to young plantlets. The plantlets were acclimatized to autoclaved soil rite, after which they were transferred to the green house. Among the survived plantlets, 10 plants for each primer were randomly selected for inter‐simple sequence repeat (ISSR) analysis. Of the 10 primers tested, 5 produced reproducible and monomorphic bands, 2 led to minor variation with the appearance of unique bands, and the remaining 3 did not show any banding pattern. The majority of the regenerants had similar characteristics to the donor plant, suggesting genetic homogeneity of in vitro raised plants.

Small RNA and Transcriptome Sequencing Reveal a Potential miRNA-Mediated Interaction Network That Functions during Somatic Embryogenesis in Lilium pumilum DC. Fisch.

“…SE has been reported in Arabidopsis thaliana (Gaj, 2001), Phoenix dactylifera L. (Naik and Al-Khayri, 2016), Theobroma cacao L. (Ajijah et al, 2016), Gladiolus hybridus (Wu J. et al, 2015), and Cassia occidentalis L. (Naz et al, 2015). However, the understanding of the mechanism underlying the formation of somatic embryos is still in its infancy, and SE remains the least understood pattern of regeneration (Fehér, 2015).…”

Genetic diversity among mulberry genotypes from seven countries

“…ISSRs are based on microsatellite DNAs, which have high levels of mutations in non-coding regions due to replication slippage, and, therefore, these mutations can accumulate and differ between varieties in different populations (Zietkiewicz et al 1994). Other than applications to genetic stability and gene variation (Qian et al 2001;Naz et al 2016), ISSRs have higher efficiencies in polymorphism analyses in plants (Nagaoka and Ogihara 1997;Devarumath et al 2002;Zargar et al 2016). However, AFLPs require known flanking sequence and advanced digestion, respectively.…”

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

“…Molecular analyses and flow cytometry have been used in many species to evaluate the trueness-to-type of plantlets regenerated via somatic embryogenesis. These species include olives (Brada€ ı et al, 2016), muskmelon (Raji et al, 2018), chrysanthemum (Lema-Rumi nska and Sliwi nska, 2015), ajowan (Niazian et al, 2017), Italian grapevine (Carra et al, 2016), Anthurium andraeanum (Bhattacharya et al, 2016), citrus species (Meziane et al, 2017), and Cassia occidentalis (Naz et al, 2016). In walnut, RFLP and isozyme analysis were used to determine the origin of SEs by Aly et al (1992).…”