Solving Cell Infiltration Limitations of Electrospun Nanofiber Meshes for Tissue Engineering Applications

Guimarães, Alexandre Caixeta; Martins, Albino; Pinho, Eva; Faria, Susana; Reis, Rui L.; Neves, Nuno M.

doi:10.2217/nnm.10.31

Cited by 76 publications

(62 citation statements)

References 54 publications

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“…Due to their unique properties, such nanofibers can be used as drug delivery systems or tissue scaffolds or both. Anyway, their design should be governed by the final characteristics required for specific applications (Guimaraes et al, 2010).…”

Section: Nanofibers For Biomedical Usementioning

confidence: 99%

Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

Pelipenko

Kocbek

Kristl

2015

International Journal of Pharmaceutics

186

125

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Section: Nanofibers For Biomedical Usementioning

confidence: 99%

Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

Pelipenko

Kocbek

Kristl

2015

International Journal of Pharmaceutics

186

125

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“…The major criticism against the use of electrospun polymeric nanofibers in tissue engineering is their small pore size and thereby the fish-net effect, which restricts the infiltration of cells and vasculature. 25,26 However, wafers of functional cells in microscale thickness (50-100 mm, the stretchable length of a cell) can be successfully developed using biodegradable nanofibrous wafer discs and appropriate cell seeding strategy. Similarly, porous microfibrous and ceramic discs can be developed in microscale thickness by appropriate methods and can be used as suitable platform for tissue/vessel ingrowth after appropriate bioengineering.…”

Section: Introductionmentioning

confidence: 99%

Bone Tissue Engineering with Multilayered Scaffolds—Part I: An Approach for Vascularizing Engineered Constructs In Vivo

Sathy

Mony

Menon

et al. 2015

Tissue Engineering Part A

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Obtaining functional capillaries through the bulk has been identified as a major challenge in tissue engineering, particularly for critical-sized defects. In the present study, a multilayered scaffold system was developed for bone tissue regeneration, designed for through-the-thickness vascularization of the construct. The basic principle of this approach was to alternately layer mesenchymal stem cell-seeded nanofibers (osteogenic layer) with microfibers or porous ceramics (osteoconductive layer), with an intercalating angiogenic zone between the two and with each individual layer in the microscale dimension (100-400 mm). Such a design can create a scaffold system potentially capable of spatially distributed vascularization in the overall bulk tissue. In the cellular approach, the angiogenic zone consisted of collagen/fibronectin gel with endothelial cells and pericytes, while in the acellular approach, cells were omitted from the zone without altering the gel composition. The cells incorporated into the construct were analyzed for viability, distribution, and organization of cells on the layers and vessel development in vitro. Furthermore, the layered constructs were implanted in the subcutaneous space of nude mice and the processes of vascularization and bone tissue regeneration were followed by histological and energy-dispersive X-ray spectroscopy (EDS) analysis. The results indicated that the microenvironment in the angiogenic zone, microscale size of the layers, and the continuously channeled architecture at the interface were adequate for infiltrating host vessels through the bulk and vascularizing the construct. Through-thethickness vascularization and mineralization were accomplished in the construct, suggesting that a suitably bioengineered layered construct may be a useful design for regeneration of large bone defects.

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“…More fascinating is the nanofibrous network that is considered to mimic the native ECM fibers [185][186][187]. However, the line-ofsight process creates a very thin membrane structure, which is thus considered 'pseudo-3D' (rather than 'exactly 3D') structure, holding some limitations in completely mimicking the native 3D architecture and in progressive cellular penetration [176][177][178][179]188]. Even so, the electrospun nanofibers have been potentially used to cultivate stem cells to provide ECM mimic matrix and to interpret the cellular interactions with the underlying ECM-like nanostructure [185,189,190].…”

Section: D Geometrical Scaffoldsmentioning

confidence: 99%

Biomaterials control of pluripotent stem cell fate for regenerative therapy

Pérez

Choi

Han

et al. 2016

Progress in Materials Science

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Solving Cell Infiltration Limitations of Electrospun Nanofiber Meshes for Tissue Engineering Applications

Abstract: An electrospun mesh was created with sufficient pore size to allow cell infiltration into its structure, thus resulting in a fully populated construct appropriate for 3D tissue engineering applications.

Cited by 76 publications

References 54 publications

Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration

Bone Tissue Engineering with Multilayered Scaffolds—Part I: An Approach for Vascularizing Engineered Constructs In Vivo

Biomaterials control of pluripotent stem cell fate for regenerative therapy

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