Biomaterials control of pluripotent stem cell fate for regenerative therapy

Pérez, Román A.; Choi, Seong–Jun; Han, Cheol-Min; Kim, Jung‐Ju; Shim, Hosup; Leong, Kam W.; Kim, Hae‐Won

doi:10.1016/j.pmatsci.2016.05.003

Cited by 39 publications

(21 citation statements)

References 274 publications

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“…Conventional 2D coculture system could not replicate the 3D environment in vivo, and many approaches for 3D coculture were developed (27). The Transwell method provided a simple model to culture different types of cells in a noncontact way for routine use.…”

Section: Discussionmentioning

confidence: 99%

3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

et al. 2020

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The integration of structure and function for tissue engineering scaffolds is of great importance in mimicking native bone tissue. However, the complexity of hierarchical structures, the requirement for mechanical properties, and the diversity of bone resident cells are the major challenges in constructing biomimetic bone tissue engineering scaffolds. Herein, a Haversian bone–mimicking scaffold with integrated hierarchical Haversian bone structure was successfully prepared via digital laser processing (DLP)–based 3D printing. The compressive strength and porosity of scaffolds could be well controlled by altering the parameters of the Haversian bone–mimicking structure. The Haversian bone–mimicking scaffolds showed great potential for multicellular delivery by inducing osteogenic, angiogenic, and neurogenic differentiation in vitro and accelerated the ingrowth of blood vessels and new bone formation in vivo. The work offers a new strategy for designing structured and functionalized biomaterials through mimicking native complex bone tissue for tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

et al. 2020

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“…A). However, in addition to the expensive and time‐consuming in vitro cell expansion procedures, several other technical hurdles must be addressed before the clinical utility of such stem cell therapies for combating human diseases can be realized . As an alternative to cell transplantation, tissue regeneration can also be achieved using a cell‐free approach that obviates the need for delivering stem cells from an exogenous source, thereby qualifying this technique for broader applications (Fig.…”

Section: Steering Endogenous Cell Populations For Therapeuticsmentioning

confidence: 99%

Administration of signalling molecules dictates stem cell homing for in situ regeneration

Yin

et al. 2017

J Cellular Molecular Medi

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Ex vivo‐expanded stem cells have long been a cornerstone of biotherapeutics and have attracted increasing attention for treating intractable diseases and improving tissue regeneration. However, using exogenous cellular materials to develop restorative treatments for large numbers of patients has become a major concern for both economic and safety reasons. Advances in cell biological research over the past two decades have expanded the potential for using endogenous stem cells during wound healing processes, and in particular, recent insight into stem cell movement and homing has prompted regenerative research and therapy based on recruiting endogenous cells. Inspired by the natural healing process, artificial administration of specific chemokines as signals systemically or at the injury site, typically using biomaterials as vehicles, is a state‐of‐the‐art strategy that potentiates stem cell homing and recreates an anti‐inflammatory and immunomodulatory microenvironment to enhance in situ tissue regeneration. However, pharmacologically coaxing endogenous stem cells to act as therapeutics in the field of biomedicine remains in the early stages; its efficacy is limited by the lack of innovative methodologies for chemokine presentation and release. This review describes how to direct the homing of endogenous stem cells via the administration of specific signals, with a particular emphasis on targeted signalling molecules that regulate this homing process, to enhance in situ tissue regeneration. We also provide an outlook on and critical considerations for future investigations to enhance stem cell recruitment and harness the reparative potential of these recruited cells as a clinically relevant cell therapy.

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“…Novel strategies involve the continuous adaptation of the inserted matrices to trigger a series of biological processes that may ultimately lead to bone regeneration (Pérez et al, 2013 ). These processes will mainly stimulate cells, through specific intrinsic features provided by the biomaterial and/or the release of extrinsic factors allocated within the substrate, such as molecules with biological activity (Perez et al, 2016 ). Among the different steps, the most critical are mitigation of the possible bacterial infection, the control of the initial inflammatory response, the attraction of cells to the site of defect through the formation of blood vessels, and the final maturation of the bone tissue (Perez et al, 2015b ).…”

Section: Introductionmentioning

confidence: 99%

Biological Roles and Delivery Strategies for Ions to Promote Osteogenic Induction

Bosch-Rué

Díez-Tercero

Giordano-Kelhoffer

et al. 2021

Front. Cell Dev. Biol.

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Bone is the most studied tissue in the field of tissue regeneration. Even though it has intrinsic capability to regenerate upon injury, several pathologies and injuries could hamper the highly orchestrated bone formation and resorption process. Bone tissue engineering seeks to mimic the extracellular matrix of the tissue and the different biochemical pathways that lead to successful regeneration. For many years, the use of extrinsic factors (i.e., growth factors and drugs) to modulate these biological processes have been the preferred choice in the field. Even though it has been successful in some instances, this approach presents several drawbacks, such as safety-concerns, short release profile and half-time life of the compounds. On the other hand, the use of inorganic ions has attracted significant attention due to their therapeutic effects, stability and lower biological risks. Biomaterials play a key role in such strategies where they serve as a substrate for the incorporation and release of the ions. In this review, the methodologies used to incorporate ions in biomaterials is presented, highlighting the osteogenic properties of such ions and the roles of biomaterials in controlling their release.

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Biomaterials control of pluripotent stem cell fate for regenerative therapy

Cited by 39 publications

References 274 publications

3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

Administration of signalling molecules dictates stem cell homing for in situ regeneration

Biological Roles and Delivery Strategies for Ions to Promote Osteogenic Induction

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