Soluble antigens associated with infection with apple chlorotic leaf spot virus

Chairez, Ruben; Lister, R. M.

doi:10.1016/0042-6822(73)90160-8

Cited by 14 publications

(4 citation statements)

References 11 publications

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“…Such antigens have also been reported for other elongate viruses, e.g. for apple chlorotic leaf spot virus (CLSV) by Chairez and Lister (1973). These authors also found NaCl (0.85~) essential for serology in agar gel.…”

Section: Discussionmentioning

confidence: 84%

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Further characterization of celery latent virus

Bos¹,

Díaz-Ruíz²,

Maat³

1978

Netherlands Journal of Plant Pathology

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Celery latent virus has been isolated from plants of celeriac (Apium graveolens var. rapaceum). Chenopodium amaranticolor and C. quinoa are good assay plants. Celery (A. graveolens var. dulce) and celeriac (13 cultivars tested) did not react with visible symptoms.Fourteen new artificial hosts were found. New systemic symptomless hosts are Anthriscus eerefolium, Nicoliana megalosiphon, Pisum sativum, Spinacia oleracea and Trifolium incarnatum.Systemic symptoms were caused in the pea cultivars Cicero and Dark Skin Perfection.Five aphid species, including Cavariella aegopodii, four of which not tested with the virus before, were unable to transmit the virus.Seed transmission was confirmed in celeriac (up to 34~o) and in C. quinoa (up to 67~) and detected for the first time in Amaranthus caudatus. Detection is easier in seedlings and germinated seeds than in dry seeds.Results of purification were erratic but best at high pH (8 or 9). Sedimentation coefficient was 161 S.An antiserum reacted with purified virus in micro-precipitin tests (titer 256) but, especially at high salt concentrations also in agar gel (titer 64), presumably because of easy degradation of virus protein. Reactions in agar gel also occurred with crude extracts from virus-infected C. quinoa and celeriac.With the electron microscope flexuous virus particles were found in low concentration in crude sap and in high concentration in purified preparations. Particle measurements revealed an average length of 885 nm (in one preparation 940 nm).Light microscopy did not show inclusion bodies in epidermal strips nor did electron microscopy of ultrathin sections reveal pinwheels and other structures typical of the potyvirus group. The virus evidently belongs to a new morphological group, possibly together with some other viruses hitherto insufficiently studied.The virus seems of potential economic importance only, but it is advised to use virus-free mother plants of celery for seed production.

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Section: Discussionmentioning

confidence: 84%

“…For CLSV also the best culture host was C. quinoa, however with similar problems in purification and low virus yields (cf. Chairez and Lister, 1973). In other respects CLSV differs from CeLV, such as in shorter particles (ca.…”

Section: Discussionmentioning

confidence: 93%

Further characterization of celery latent virus

Bos¹,

Díaz-Ruíz²,

Maat³

1978

Netherlands Journal of Plant Pathology

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“…CLSV isolates in C. quinoa sap formed single precipitin lines with antisera to CLSV-A, an apple isolate described by Lister & Hadidi (1971) and CLSV-S, an antiserum prepared from the soluble antigen of CLSV-A (Chairez & Lister, 1973). The precipitates were continuous with those of CLSV isolates from apple and pear with no spur formation or crossing over of the precipitate lines.…”

Section: Apple Chlorotic Leclfspot Virusmentioning

confidence: 81%

Hedgerow hawthorn (Crataegus spp.) and blackthorn (Prunus spinosa) as hosts of fruit tree viruses in Britain

Sweet

1980

Annals of Applied Biology

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S U M M A R YApple chlorotic leafspot virus (CLSV) was detected in 27 of 109 hawthorn and three of 67 blackthorn plants sampled in various parts of Britain. The CLSV isolates possessed similar properties to those isolated from other rosaceous species but differed in the severity of symptoms they induced in woody indicators. No seed or aphid transmission of CLSV was detected.Prunus necrotic ringspot (PNRV) and prune dwarf (PDV) viruses were detected in four and three respectively of 67 blackthorn plants. The PNRV and PDV isolates were serologically closely related to isolates from cherry. Arabis mosaic virus was detected in one blackthorn plant, but plum pox virus was not found in any of the tested plants.

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“…Preliminary analysis indicated that B W protein has no tryptophan, about 5 tyrosine residues and about 5% cystine residues, and the estimated specific absorbance of BYV protein at 280 nm is about 0.356 OD per mg (Rees, Short, Bar-Joseph, and Hull, unpublished). The size of the coat protein of CTV (Bar-Joseph et al 1972), CLSV (Chairez and Lister, 1973), B W ( Bar-Joseph and Hull, 1974) and CYFV (Bar-Joseph and Smookler, 1976) as determined by sodium dodecyl sulphate (SDS) acrylamide gel electrophoresis, is summarized in table 3. The differences in weights are small and perhaps reflect different experimental conditions.…”

Section: Particle Characterization Tablementioning

confidence: 99%