Comparison of Particle Characteristics and Cytopathology of Citrus Tristeza Virus and Other Morphologically Similar Viruses

Complex Citrus tristeza virus (CTV) populations composed of mixtures of different strains of the virus are commonly found in citrus trees in the field. At present, little is known about how these populations are formed, maintained, and how they are structured within a host. Here we used a novel in situ hybridization approach allowing simultaneous visualization of two different RNA targets with high sensitivity and specificity to examine the distribution of two isolates, T36 and T68-1, representing phylogenetically distinct strains of CTV, in a citrus host in single and mixed infections. Remarkably, in doubly inoculated plants the two virus variants appeared to be well mixed within the infected tissue and showed no spatial segregation. In addition, both CTV variants were often found occupying the same cells. Possible mechanisms involved in shaping CTV populations and the biological significance of the observed lack of structural separation of the individual components are discussed.

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Comparison of Particle Characteristics and Cytopathology of Citrus Tristeza Virus and Other Morphologically Similar Viruses

Abstract: Threadlike particles (TLP) about 2000 x 10-1 2 nm in size were found by Katajima et al. (1974) to be associated with tristeza virus (CTV) infection. Although completion of Koch's postulates with TLP has not yet been achieved, several lines of evidence (

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References 19 publications

Interspecific Periclinal Chimeras as a Strategy for Cultivar Development

Interspecific Periclinal Chimeras as a Strategy for Cultivar Development

Cytopathology

Simultaneous visualization of two Citrus tristeza virus genotypes provides new insights into the structure of multi-component virus populations in a host

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