Cytopathology

Lesemann, D.-E.

doi:10.1007/978-1-4684-7038-3_7

Cited by 48 publications

(33 citation statements)

References 145 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2a, b and c). These inclusion bodies were similar in appearance to the rod-like elements reportedly found in the cytoplasm of PVY-infected cells (Edwardson, 1974;Christie & Edwardson, 1977;Lesemann, 1988). The specificity of the labelling was shown by, first, the specific labelling of amorphous inclusions in PVYinfected cells by PVY HC antibodies (Fig.…”

supporting

confidence: 73%

“…In this paper, using immunogold techniques, we report that the HC protein of PVY is localized in the rod-shaped cytoplasmic inclusions (Edwardson, 1974;Christie & Edwardson, 1977) produced by this virus in infected cells. These types of inclusions are categorized as 'amorphous inclusions' (Lesemann, 1988) although morphologically the rod-like inclusions found in PVY-infected plants are not similar to those found in other potyviruses such as papaya ringspot (MarteUi & 0000-9476 © 1990SGM Russo, 1976 or pepper mottle virus (Edwardson, 1974). Apparently, the amorphous inclusions produced by different potyviruses take on different morphological forms.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Potato virus Y helper component protein is associated with amorphous inclusions

Baunoch

Das

Hari

1990

Journal of General Virology

View full text Add to dashboard Cite

supporting

confidence: 73%

mentioning

confidence: 99%

Potato virus Y helper component protein is associated with amorphous inclusions

Baunoch

Das

Hari

1990

Journal of General Virology

View full text Add to dashboard Cite

“…Vesicular bodies of various sizes and shapes are cytopathological structures associated with infections of many different types of plant viruses, and in some cases these have been shown to contain viral dsRNAs and\or RNA-dependent RNA polymerases (Burgyan et al, 1996 ;Martelli et al, 1984 ;De Graaff & Jaspars, 1994 ;Lesemann, 1988). These observations and the fact that LIYV RNA 1 is alone competent for replication and primarily encodes proteins predicted to be involved in replication (Klaassen et al, 1995), further support the idea that the vesicles induced by LIYV are associated with LIYV RNA replication.…”

mentioning

confidence: 77%

“…Previous studies have shown that closterovirus infections induce characteristic inclusion bodies within phloemassociated cells, including phloem parenchyma and companion cells (Lesemann, 1988). These inclusion bodies include cytoplasmic vesiculated membranous areas within infected cells, referred to as BYV-type vesicles (beet yellows virus ; Francki et al, 1985), surrounded by lipid droplets.…”

mentioning

confidence: 99%

“…However, by comparing protoplasts infected with LIYV RNA 1 with those infected with LIYV RNAs 1 and 2, we found that infection by only LIYV RNA 1 is sufficient to induce formation of the typical BYV-type vesiculated inclusion bodies. These vesiculated inclusion bodies are recognized as characteristic of plant infections by closteroviruses (viruses in the family Closteroviridae) and have been identified in plants infected with aphid-, mealybug-and whitefly-transmitted closteroviruses (for a review see Lesemann, 1988). Their origins, whether derived from specific cellular organelles or generated de novo,…”

mentioning

confidence: 99%

See 1 more Smart Citation

Specific inclusion bodies are associated with replication of lettuce infectious yellows virus RNAs in Nicotiana benthamiana protoplasts.

Medina

Tian

Wierzchos

et al. 1998

Journal of General Virology

View full text Add to dashboard Cite

Nicotiana benthamiana mesophyll protoplasts, either mock-inoculated or inoculated using in vitro transcripts derived from lettuce infectious yellows virus (LIYV) RNA 1-and/or RNA 2-cloned cDNAs were analysed by transmission electron microscopy (TEM) and, in some cases, also by immunogold labelling. TEM revealed the main cytopathological effects of LIYV infections in N. benthamiana protoplasts infected with RNAs 1 and 2 : (a) typical closterovirus-induced (beet yellows virus-type) accumulations of vesiculated cytoplasmic membranes as inclusion bodies, sometimes with associated virions ; (b) scattered aggregations of virions within the cytoplasm ; and (c) electron-dense plasmalemma deposits. These were not seen in mock-inoculated protoplasts. Protoplasts inoculated only with LIYV RNA 1 contained vesiculated cytoplasmic inclusion bodies, but not virions or plasmalemma deposits. Thus, infection by only LIYV RNA 1 is sufficient to induce characteristic closterovirus vesiculated cytoplasmic inclusion bodies. However, both LIYV RNAs 1 and 2 are needed for production of virions and plasmalemma deposits.Lettuce infectious yellows virus (LIYV) is the type member of the genus Crinivirus of the family Closteroviridae. The LIYV genome is bipartite, divided among two ssRNAs of 8118 and 7193 nucleotides (RNAs 1 and 2 respectively), giving a total of 15 311 nucleotides (Klaassen et al., 1995). On LIYV RNA 2 is the ' hallmark closterovirus gene array ' including five genes encoding : a small hydrophobic protein of as yet unknown function (ORF 1) ; an HSP70 homologue (ORF 2) ; a protein of unknown function (p59, ORF 3) ; the capsid protein (CP,

show abstract

Potexviruses and Carlaviruses – Short Filamentous Viruses

Martelli¹,

Rubino²

2012

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Potexviruses and carlaviruses are plant‐infecting viruses belonging to the family Aphaflexiviridae and Betaflexiviridae , respectively. Both have slightly flexuous filamentous particles measuring 470–580×13 nm (potexviruses), and 610–700×12–15 nm (carlaviruses). Virions contain 94–95% protein and 5–6% positive sense, single‐stranded ribonucleic acid c . 5800–7000 nucleotides (nt) (potexviruses) and 7900–9000 nt (carlaviruses) in size. Both virus types have a genome with a similar structural organisation, comprising five (potexviruses) or six (carlaviruses) open reading frames, three of which, known as the ‘triple gene block’, encode polypeptides that facilitate virus movement within the host. Natural hosts of both virus types are herbaceous (weeds, ornamentals, vegetables) rather than woody plants. Many potexviruses are agents of severe diseases, whereas a high percentage of carlaviruses induces symptomless infections. Transmission of potexviruses in nature is, by and large, by mechanical contact, whereas carlaviruses are transmitted primarily by aphids with a nonpersistent modality. Key Concepts: Potexviruses and carlaviruses infect primarily herbaceous hosts after mechanical or vector‐driven transmission, respectively. Particles of both genera are filamentous and slightly flexuous and accumulate in aggregates in the cytoplasm of parenchyma cells. Demarcation of species within the genera takes into account molecular and biological criteria. Both genera are characterised by the presence of the so‐called triple gene block that provides the support for virus movement in infected hosts. Potexviruses and carlaviruses constitute models for virus replication, movement and encapsidation studies. Control is based on preventive measures, such as sanitary selection, production of virus‐free seed lots and resistance through conventional breeding and genetic engineering.

show abstract

Cytopathology

Cited by 48 publications

References 145 publications

Potato virus Y helper component protein is associated with amorphous inclusions

Potato virus Y helper component protein is associated with amorphous inclusions

Specific inclusion bodies are associated with replication of lettuce infectious yellows virus RNAs in Nicotiana benthamiana protoplasts.

Potexviruses and Carlaviruses – Short Filamentous Viruses

Contact Info

Product

Resources

About