Hedgerow hawthorn (<i>Crataegus</i> spp.) and blackthorn (<i>Prunus spinosa</i>) as hosts of fruit tree viruses in Britain

Sweet, Jeremy

doi:10.1111/j.1744-7348.1980.tb03899.x

Cited by 19 publications

(5 citation statements)

References 17 publications

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“…Apple chlorotic leaf spot virus (ACLSV), the type member of the genus Trichovirus , is an important plant pathogen due to its worldwide distribution and broad host range, which includes cultivated, ornamental and wild species within the family Rosaceae (Al Rwahnih et al ., ). Even though it is often latent in cultivated pome fruit species, ACLSV causes serious diseases in stone fruit trees, while its presence has also been established in several wild and ornamental species (Peña‐Iglesias & Ayuso, ; Sweet, , ,b; Rana et al ., ). The complete genome sequence of several ACLSV isolates has been determined (German‐Retana et al ., ; Marini et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Host‐range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species

et al. 2013

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A large-scale survey was carried out to study the host range and genetic diversity of Apple chlorotic leaf spot virus (ACLSV) in various Rosaceae species, with a special emphasis on ornamentals and wild shrubs. Samples were tested by DAS-ELISA using two different antisera, and RT-PCR amplification of part of the CP gene. There was generally a poor correlation between the results obtained with the two sets of serological reagents and between serological and molecular detection assays. Using a nested RT-PCR assay developed here, ACLSV was found to be widespread among cultivated, ornamental and wild species of the Rosaceae. The virus was detected for the first time in plum, wild cherry, Crataegus monogyna, Prunus spinosa and Prunus cerasifera in Greece. Sequences of a part of the CP encoding gene and the 3′ untranslated region from ACLSV isolates originating from various wild species and ornamentals were compared to those of isolates from cultivated hosts, showing similar divergence levels. Further phylogenetic analysis using the sequenced region indicated that the isolates from wild or ornamental hosts were not more closely related to each other than to isolates from cultivated hosts. The possible role of different factors in the spread of ACLSV on cultivated, ornamental and wild species is discussed.

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Section: Introductionmentioning

confidence: 99%

Host‐range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species

et al. 2013

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“…1). This is the first characterization of ACLSV infecting pear plants in Taiwan. ACLSV infections by mechanical inoculation have been reported in apple (Lister et al, 1965;Saksena and Mink, 1969), pear (Nemec, 1967;Pfaeltzer, 1962), plum and hawthorn (Sweet, 1980), cherry (Everett et al, 1993;Rana et al, 2008b), almond (Spiegel et al, 2005), and quince (Mathioudakis et al, 2007;Rana et al, 2007). It seems that the host range of ACLSV LTS1 is different from that of other reported ACLSV isolates.…”

Section: Discussionmentioning

confidence: 97%

Biological and Molecular Characterization of Apple chlorotic leaf spot virus Causing Chlorotic Leaf Spot on Pear (Pyrus pyrifolia) in Taiwan

Wu¹,

Ku²,

Chen³

et al. 2010

horts

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Pear plants (Pyrus pyrifolia var. Hengshen) showing symptoms of chlorotic spots on leaves were observed in orchards in central Taiwan in 2004. The sap of diseased leaves reacted positively to Apple chlorotic leaf spot virus (ACLSV) antiserum. A purified virus isolate (LTS1) from pear was characterized by host range, electron microscopy, phylogenetic analyses, serological property, and back-inoculation experiments to pear. Fifteen of 28 species of tested plants were susceptible to this virus after mechanical inoculation. Pathogenicity of ACLSV isolate LTS1 was verified by back-inoculating to pear seedlings. Filamentous virions of ≈12 × 750 nm were observed in the preparations of purified virus. Virus particles accumulated in the cytoplasm were observed in the ultrathin sections of LTS1-infected pear leaf tissue. Sequence analyses of the coat protein (CP) gene of LTS1 and the CP gene of LTS2, which originated from a distinct symptomatic pear sample, shared 81.4% to 92.6% nucleotide and 87.6% to 98.4% amino acid identities with those of the CP of 35 ACLSV isolates available in GenBank. ACLSV isolates were grouped into four clusters, i.e., Asia I, II, III, and Europe, and isolates LTS1 and LTS2 were classified as members of cluster Asia II and Asia I, respectively, based on phylogenetic data. Moreover, the variability of amino acid sequences of the CP gene of 37 ACLSV isolates showed geographically associated clustering in the phylogenetic tree. To our knowledge, this is the first study on the characterization of ACLSV causing the leaf chlorotic spot disease of pear in Taiwan. This study also provides the phylogenetic relationships among ACLSV populations based on amino acid sequences of CPs, which are correlated with their geographic origins.

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“…Previously, ACLSV has been detected in apple and quince seedlings (Posnette & Cropley, 1964). ACLSV has also been detected in hedgerow‐grown hawthorn and blackthorn seedlings in the United Kingdom (Sweet, 1980). Mazeikiene et al (2018) reported that ACLSV occurs in old apple seedling trees.…”

Section: Discussionmentioning

confidence: 99%

“…ACLSV has been detected in hedges of Prunus spinosa and hawthorn seedlings ( Crataegus spp.) (Sweet, 1980). The only experimentally proven transmission pathway is through vegetative propagation of infected planting material (Šutić et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and phylogenetic relationships of apple chlorotic leaf spot virus isolates from Malus, Pyrus and Prunus hosts in Latvia

2023

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Apple chlorotic leaf spot virus (ACLSV, genus Trichovirus, subfamily Trivirinae, family Betaflexiviridae) is a single-stranded, positive-sense RNA virus, 7474-7561 nucleotides (nt) long, excluding the poly-A tail at the 3′ end (Yoshikawa & Takahashi, 1988). The virus genome consists of three overlapping open reading frames (ORFs): ORF1 encodes the large replication-associated proteins, which are directly expressed from genomic RNA, whereas ORF2 and ORF3 are expressed via subgenomic RNAs (sgRNAs) and encode the movement protein (MP) and the viral coat protein (CP), respectively (German et al., 1990;Sato et al., 1993). At first, ACLSV was described as the common latent virus of Malus spp. (Mink & Shay, 1959). Later, it was found to occur in commercial pome and stone fruit species, as well as in ornamental and wild Rosaceae plants (Cropley et al., 1963;Katsiani et al., 2014). The natural host range of ACLSV exceeds more than 35 species (Katsiani et al., 2014;Šutić et al., 1999). Most of them are commercially grown hosts: apples, apricots, cherries, peaches, plums and pears. Other ornamental and fruit crop plant species of the Rosaceae family: almond (Prunus dulcis), serviceberry (Amelanchier spp.), hawthorn (Crataegus spp.), loquat (Eriobotrya japonica), medlar (Mespilus germanica), mountain ash (Sorbus aucuparia), quince (Cydonia oblonga) and Japanese quince (Chaenomeles

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Hedgerow hawthorn (Crataegus spp.) and blackthorn (Prunus spinosa) as hosts of fruit tree viruses in Britain

Cited by 19 publications

References 17 publications

Host‐range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species

Host‐range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species

Biological and Molecular Characterization of Apple chlorotic leaf spot virus Causing Chlorotic Leaf Spot on Pear (Pyrus pyrifolia) in Taiwan

Genetic diversity and phylogenetic relationships of apple chlorotic leaf spot virus isolates from Malus, Pyrus and Prunus hosts in Latvia

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