Further characterization of celery latent virus

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Parsley latent virus, a hitherto undescribed virus, was isolated from 38 out of 54 samples of seed of parsley (Petroselinum crispum) of 17 out of 24 cultivars and from all five European countries tested, but not from some samples from the USA. It could easily be detected in seedlings and also in seeds germinated on moist filter paper, but not in dry seeds or in seeds soaked in water. Strawberry latent ringspot virus was detected in five samples. The parsley virus is symptomless in parsley and caused latent systemic infection in Gomphrena globosa, three cultivars of Spinacia oleracea and weak and often transient systemic symptoms in Chenopodium amaranticolor, C. giganteum, C. glaucum and C. quinoa, but did not infect any other species out of all 32 species of seven plant families tested in total.The virus could easily be transmitted mechanically but not by seven aphid species in the non-persistent manner. Dilution end-point was between 100 and 1000, thermal inactivation between 55 and 60~ and ageing in vitro between 7 and 10 days.Purification yielded a single infectious component. The particles were spherical, ca. 27 nm in diameter, with a sedimentation coefficient of 127.5 S, a buoyant density of 1.449 g/ml, an RNA content of 36% and one type of protein with a relative molecular mass of 22 x 103. Purification without Triton and urea resulted in preparations with aggregates each consisting of 12 particles in icosahedral array.The virus differs from all viruses described so far and did not show clear serological affinity with antisera to any of 34 widely differing viruses tested. It does not seem of direct practical importance and may be easily overlooked.

Section: Introductionmentioning

confidence: 97%

Parsley latent virus, a new and prevalent seed-transmitted, but possibly harmless virus of Petroselinum crispum

Bos¹,

Huttinga²,

Maat³

1979

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“…After back-inoculation the virus did not produce symptoms in celery or in 13 cultivars of celeriac. It was later identified as the ungrouped seedtransmitted celery latent virus and it was found not to enhance the symptoms of celery mosaic virus in celery (Bos et al, 1978).…”

Section: Time Of Infectionmentioning

confidence: 98%

“…Preliminary reports on celery mosaic potyvirus (CeMV) as the causal virus and on its incidence and distribution were by Bos (1973) and Mandersloot et al (1973), respectively. The seed-borne celery latent virus (CeLV), also found in celeriac, has been further characterized (Bos et al, 1978), and data on 18 viruses other than CeMV found in umbelliferous crops and wild species in the Netherlands, when seeking infection sources of CeMV, are in press (Van Dijk and Bos, 1989).…”

Section: Introductionmentioning

confidence: 99%

An epidemic of celery mosaic potyvirus in celeriac (Apium graveolens var. rapaceum) in the Netherlands

Bos¹,

Mandersloot²,

Vader³

et al. 1989

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An annually recurring virus epidemic has caused severe damage and sometimes total yield loss in crops of celeriac in the south-west of the Netherlands for several years since 1969. Celery latent virus, cucumber mosaic cucumovirus and tobacco rattle tobravirus were isolated from diseased plants, but a potyvirus was the most prevalent virus present. It did not cause local lesions in Chenopodium amaranticolor or C. quinoa and did not infect other non-Umbelliferae.The virus was identified as celery mosaic potyvirus and confirmed to be the causal agent.Field surveys from 1971 to 1977 showed a rapid increase of disease incidence in consecutive years, soon leading to near-abandonment of the crop in the original centre of cultivation and its northward translocation, gradually followed by the disease. Incidence in fields often increased from zero by the end of July, when symptoms first appeared, to 100% early during September. In 1976 final incidence was 75 to 100% in 41~ of the fields inspected. Temporary protection with aphid-proof cages showed that first infection occurred about three weeks before symptoms appear and not before the beginning of July, and that the virus may still spread after late September.When testing samples from other umbelliferous crops and wild species near infested crops, several viruses were detected but not celery mosaic virus. The virus may be introduced from yet unknown distant sources, as also suggested by the pattern of spread in crops of celeriac.Celeriac cultivars differ considerably in resistance to the virus, but resistant cultivars generally are of poorer quality for consumption and processing than the original highly sensitive cultivar. In recent years cultivation has recovered considerably with the advent of new cultivars.

“…CeLV has been found in the seventies in seeds and crops of celeriac (Bos, 1973;Bos et al, 1978), celery and leaf celery. Samples tested recently only incidentally contained the virus.…”

Section: Celery Latent Virusmentioning

confidence: 98%

“…The seed-borne celery latent virus (CeLV) (Bos et al, 1978) and parsley latent virus (PILV) (Bos et al, 1979) were accidentally detected in plants of celeriac and parsley, respectively, in which they do not seem to cause economic damage.…”

Section: Introductionmentioning

confidence: 99%

Survey and biological differentiation of viruses of wild and cultivated umbelliferae in the Netherlands

Dijk¹,

Bos²

1989

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