Sol–gel encapsulated glucose oxidase arrays based on a pH sensitive fluorescent dye

Yildirim, Nimet; Demirkol, Dilek Odaci; Ozturk, Gulsiye; Alp, Serap; Ergün, Yavuz; Dornbusch, Kay; Feller, K.‐H.; Tımur, Suna

doi:10.1016/j.dyepig.2010.10.003

Cited by 14 publications

(5 citation statements)

References 18 publications

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“…The measuring principle is the same as in every color measurement system 38, 39. The light which is reflected or transmitted (in our case) passes through the filters and is transformed into a corresponding current by the wideband photo diodes.…”

Section: Methodsmentioning

confidence: 99%

Microfluidic devices and true‐color sensor as platform for glucose oxidase and laccase assays

Demirkol

Dornbusch²,

Feller³

et al. 2011

Engineering in Life Sciences

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The commonly used assay for measuring glucose oxidase (GOx) and laccase (Lac) activity, based on spectrophotometric test using 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), has been adapted to measure these enzyme activities via microfluidic devices. The effects of flow rate and concentration of substrates were examined. The developed assays allowed analyzing GOx and Lac activities in the range of 0.8–8.0 Unit (0.1–1.0 mg/mL) and 0.9–17.7 Unit (0.1–2.0 mg/mL), respectively. Moreover, repeatability tests were obtained for both enzyme assays.

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Section: Methodsmentioning

confidence: 99%

Microfluidic devices and true‐color sensor as platform for glucose oxidase and laccase assays

Demirkol

Dornbusch²,

Feller³

et al. 2011

Engineering in Life Sciences

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“…Methyltriethoxysilane (MTES) and diethoxydimethylsilane (DEDMS) are common alkyl-substituted ethoxysilane monomers, which are often used to incorporate the silicon–carbon bond into the organo-inorganic silica structure, which leads to the preparation of new materials [ 15 , 16 , 17 ]. All previous studies of our research group were performed using a compound with one non-hydrolysable bond, methyltriethoxysilane, as a hydrophobic additive to tetraethoxysilane.…”

Section: Introductionmentioning

confidence: 99%

“…It can be expected that when DEDMS is used as a precursor, a less rigid but strong shell will be formed around the cells of microorganisms. It is known [ 12 , 13 , 14 , 15 ] that the gelation time also increases upon passing from tetraethoxysilane (TEOS) to DEDMS, which may be due to the influence of the steric effect of precursors on the gelation rate.…”

Section: Introductionmentioning

confidence: 99%

The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices

et al. 2022

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Biomembranes based on an organosilica sol–gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The use of scanning electron microscopy (SEM) and the low-temperature nitrogen adsorption method (BET) showed that the sol–gel matrix forms a capsule around microorganisms that does not prevent the exchange of substrates and waste products of bacteria to the cells. The use of DEDMS as part of the matrix made it possible to increase the sensitivity coefficient of the biosensor for determining BOD by two orders of magnitude compared to a biosensor based on methyltriethoxysilane (MTES). Additionally, the long-term stability of the bioreceptor increased to 68 days. The use of such a matrix neutralized the effect of heavy metal ions on the microorganisms’ catalytic activity in the biosensor. The developed biosensor was used to analyze water samples from water sources in the Tula region (Russia).

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“…[9] Optical arrays combined with pHsensitive fluorescent dyes have also been used for the detection of glucose in conjunction with a fluorescence reader and luminescence spectrometer. [10] Since pH-sensitive fluorophores are usually restricted to a narrow pH range, it has been proposed that the combination of two or more dyes could provide an extended range of the pH measurements. [11] The long-known ability of universal indicator to report pH changes over a broad range of pHs [12,13] makes it suitable for monitoring a great variety of enzymatic reactions thus making it an interesting candidate for the development of multisensors.…”

Section: Introductionmentioning

confidence: 99%

Optical biosensors based on universal pH indicator as a reporter for quantification of clinically relevant compounds

Piletska

Chianella

Turner

et al. 2014

Journal of the Chinese Advanced Materials Society

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It was shown that universal indicator, a blend of pH-sensitive dyes which changes colour from red to purple over broad pH value range, in combination with a disposable optical sensor chip, could be used for the monitoring of physiologically important compounds. Changes in the optical properties of pH-sensitive dyes in the presence of enzyme catalysed reactions allowed quick and sensitive evaluation of the concentration of clinically important analytes such as glucose and urea. It was shown that the proposed optical biosensor could provide a sensitive and inexpensive method for the quantification of urea in plasma over a concentration range of 5À50 mM and glucose in the concentration range 1À25 mM. The system provides a generic platform that can be combined with other appropriate enzymes for the quantification of a range of physiological compounds to furnish versatile and portable clinical multisensors.

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Sol–gel encapsulated glucose oxidase arrays based on a pH sensitive fluorescent dye

Cited by 14 publications

References 18 publications

Microfluidic devices and true‐color sensor as platform for glucose oxidase and laccase assays

Microfluidic devices and true‐color sensor as platform for glucose oxidase and laccase assays

The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices

Optical biosensors based on universal pH indicator as a reporter for quantification of clinically relevant compounds

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