The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices

Abstract

Biomembranes based on an organosilica sol–gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The use of scanning electron microscopy (SEM) and the low-temperature nitrogen adsorption method (BET) showed that the sol–gel matrix forms a capsule around microorganisms that does not prevent the exchange of substrates and waste products of bacteria to the cells. The use of DEDMS as part of the matrix made it possible to increase the sensitivity coefficient of the biosensor for determining BOD by two orders of magnitude compared to a biosensor based on methyltriethoxysilane (MTES). Additionally, the long-term stability of the bioreceptor increased to 68 days. The use of such a matrix neutralized the effect of heavy metal ions on the microorganisms’ catalytic activity in the biosensor. The developed biosensor was used to analyze water samples from water sources in the Tula region (Russia).

“3-in-1” Hybrid Biocatalysts: Association of Yeast Cells Immobilized in a Sol–Gel Matrix for Determining Sewage Pollution

“…hansenii VKM Y-2482 was chosen as a suitable biomaterial for BOD biosensors due to its ability to oxidize a wide range of substrates. The utilization of sol–gel technology allows for the formation of organosilicon matrices around microorganism cells, providing protective properties against metal ions and fluctuations in acidity. ,, These inherent advantages enable the development of a biohybrid material characterized by broad substrate specificity, stable responses, and enhanced resistance to environmental stressors when compared to biomaterials composed of a single microorganism strain.…”

“…To the best of our knowledge, the immobilization of artificial associations of microorganisms in a sol–gel matrix has not been reported thus far. Numerous studies have focused on the immobilization of either individual cultures of microorganisms in organosilicate shells ,, or associations of microorganisms in other materials. , In this study, we aimed to immobilize an association of yeast cells comprising Ogataea polymorpha VKM Y-2559, Blastobotrys adeninivorans VKM Y-2677, and Debaryomyces hansenii VKM Y-2482 within organosilicate matrices of varying compositions. The goal was to create a hybrid material capable of serving as a receptor element in a biosensor for assessing the degree of sewage pollution.…”

Biohybrid silicon-organic materials architecture obtained using various structure-affecting agents

“…Cultivation of yeast cells Ogataea polymorpha VKM Y-2559 was carried out similarly to the article [35]. 50 µl of a suspension of yeast cells (1.3 ± 0.1 10 9 CFU/ml) in a phosphate buffer solution (20 mmol/l, pH 6.8) was added to 20 µl of a structure-controlling agent (1% chitosan, 5% polyvinyl alcohol or 5% polyethylene glycol) and stirred for 5 minutes (Elmi CM-70M07, Poland) to form biohybrid materials.…”

Immobilization of Microbial Cells in a Sol-Gel Matrix Using a Precursor with Two Non-Hydrolyzed Bonds

“…The formation of the bioreceptor element and the measurement of the catalytic activity of the cells were carried out according to an earlier article [20].…”

“…The resulting material was studied by the method of low-temperature oxygen adsorption (BET method) according to the description given in the article [20].…”