Sodium Nitroprusside and Gibberellin Effects on Seed Germination and Seedling Development of Grapevine (Vitis vinifera L.) Cvs. Ekşi Kara and Gök Üzüm

Kara, Zeki; Yazar, Kevser; Doğan, Osman; Vergili, Erdem

doi:10.1007/s10341-020-00497-8

Cited by 13 publications

(5 citation statements)

References 42 publications

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“…Initiation and growth of cell suspension cultures Nitric oxide (NO) is a bioactive molecule which can be generated through the breakdown of NO donor sodium nitroprusside molecules (SNP). Recently, SNP has been reported as an elicitor involved in regulating several signalling responses (Siddiqui et al, 2017;Khezerluo et al, 2018;Hao et al, 2020;Kara et al, 2020). In previous studies, many researchers have reported that SNP treatments are very effective in shoot regeneration of Chrysanthemum cultivars (Arun et al, 2017), Canscora diffusa (Subiramani et al, 2019), Antirrhinum majus (Rezaei Zafarghandi and Rahmati Joneidabad 2020) Valeriana jatamansi (Pandey et al, 2020) and callus induction in Dioscorea opposita (Xu et al, 2009), Hyoscyamus niger (Samsampour et al, 2018) Ficus religiosa (Hesami et al, 2019) Canscora diffusa (Subiramani et al, 2019) and Valeriana jatamansi (Pandey et al, 2020), Furthermore, SNP play important roles in increasing metabolites accumulation (Khezerluo et al, 2018;Pradhan et al, 2020;Tsolmon et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Mahendran

Kumar

Verma

et al. 2021

Plant Cell Tiss Organ Cult

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Gymnema sylvestre (Retz.) R.Br. ex Sm. is widely used as an e cient Ayurvedic traditional medicine for the treatment of diabetes. Phytochemical investigations of this plant showed gymnemic acids (a group of triterpenoid saponins) as the main active components. The present study aims to investigate the effectiveness of sodium nitroprusside (SNP) treatment for enhancement of cell suspension culture biomass and to evaluate their deacylgymnemic acid, gymnemagenin, gymnemic acid IV and gymnemic acid XVII contents of G. sylvestre. Callus was obtained from in vitro derived leaves of G. sylvestre on MS medium forti ed with 3.0 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 2.0 mg/L Kn (Kinetin), and the same were used further to produce cell suspension cultures. Cell suspensions were exposed to different concentrations of SNP (5, 10, 20 and 40 µM) and data were collected at 20, 30 and 40 days. Out of the tested concentrations, 20 µM SNP had the highest level of cell culture growth (398.94 ± 8.32 g/L FCW and 40.00 ± 0.75 g/L DCW) on 40-day as compared to control. High-performance liquid chromatography analysis showed that maximum accumulation of deacylgymnemic acid (5.51 mg/g DCW), gymnemagenin (2.80 mg/g DCW) and gymnemic acid XVII (2.08 mg/g DCW) in 20 µM SNP treatment which is (13.43, 13.86 and17.33 folds) higher than the respective control at 40 days exposure. This research suggests that G. sylvestre cell suspension culture with optimal SNP elicitation treatment could be used as a good strategy for the large-scale production of these secondary metabolites at the industrial level.

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Section: Resultsmentioning

confidence: 99%

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Mahendran

Kumar

Verma

et al. 2021

Plant Cell Tiss Organ Cult

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“…Seed germination is the first step to ensuring grain yield and quality ( 42 – 44 ). A sufficient germination index and postgermination growth promote subsequent cultivation ( 45 , 46 ). Moreover, bioactive GA promotes seed germination in a number of plant species ( 47 , 48 ).…”

Section: Discussionmentioning

confidence: 99%

Citric Acid in Rice Root Exudates Enhanced the Colonization and Plant Growth-Promoting Ability of Bacillus altitudinis LZP02

Jiao

et al. 2022

Microbiol Spectr

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“…Grape seeds have physical and physiological dormancy (Cadot;Miñana-Castelló;Chevalier, 2006;Val;Alvarenga;Cecon, 2010;Çelik, 2014;Generoso et al, 2019 Kara;Doğan;Vergili, 2020;Wang et al, 2022). By working with in vitro culture of mature zygotic embryos in grapevine, we were able to eliminate physical and physiological dormancy and obtain a high percentage of germination of normal seedlings, quickly and uniformly.…”

Section: In Vitro Germination Of Mature Zygotic Embryosmentioning

confidence: 97%

Overcoming germination barriers through in vitro culture of mature zygotic embryos of grapevine cultivars

Júnior

Carvalho

Generoso

et al. 2022

PCC&M

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Grape seeds have physical and physiological dormancy. Conventional germination methods are not very efficient. The in vitro culture of seeds and of mature zygotic embryos can be an alternative for overcoming germination barriers. The objective was to develop methodologies that increase seed germination and reduce the time to obtain seedlings. The following assays were developed: (1) conventional ex vitro germination, in a 2 × 3 factorial arrangement, of seeds with or without stratification for 90 days, from cultivars 'Niágara Rosada', 'Itália' and 'Red Globe'; (2) in vitro seed germination, in a 3 × 3 factorial arrangement, of the three cultivars and three explants (intact seeds, seeds sectioned in the micropyle region and seeds with a cross-section); and (3) in vitro germination of mature zygotic embryos, in a 4 × 2 factorial arrangement represented by four concentrations of mineral salts (MS, ½MS, WPM and ½WPM) and two cultivars ('Niágara Rosada' and 'Itália'). The conventional ex vitro germination assay resulted in a low seed germination for the three cultivars. The two types of section associated with in vitro culture provided an increase in seed germination. However, the highest germination rate (over 90%) in the shortest time (17 days) was achieved by the embryos grown in vitro. This study defines an efficient methodology to obtain high germination in grape seeds-over 85%-with uniformity and in a short time, using mature zygotic embryos grown in ½WPM culture medium. This methodology can accelerate grapevine breeding programs.

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Sodium Nitroprusside and Gibberellin Effects on Seed Germination and Seedling Development of Grapevine (Vitis vinifera L.) Cvs. Ekşi Kara and Gök Üzüm

Cited by 13 publications

References 42 publications

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Citric Acid in Rice Root Exudates Enhanced the Colonization and Plant Growth-Promoting Ability of Bacillus altitudinis LZP02

Overcoming germination barriers through in vitro culture of mature zygotic embryos of grapevine cultivars

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