Sobrevivência e diferenciação de protocormos de Oncidium flexuosum submetidos a tratamento com ácido peracético e colchicina

Unemoto, Lilian Keiko; Faria, Ricardo Tadeu de; Destro, Deonisio; Barbosa, Cristiane Muniz; Lone, Alessandro Borini

doi:10.4025/actasciagron.v31i3.697

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…Results confirm that chromosome doubling varies according to the type, species, genotype, exposure time, age, and application technique [8,39]. This fact was also verified in Dendrobium nobile [37,38], Oncidium flexuosum [35], and Dendrobium scabrilingue [30].…”

Section: Resultsmentioning

confidence: 58%

“…Oryzalin is another antimitotic that has been successfully used for the polyploidization of ornamental plants, despite being little explored in orchids [26]. Polyploids induction with colchicine and oryzalin has been used in several plant species, such as Cattleya intermedia [1,31], Dendrobium nobile [37,38], Oncidium flexuosum [35], Dendrobium scabrilingue [30], Dendrobium chrysotoxum [2] and Dendrobium, Cymbidium, Epidendrum, Odontioda, Phalaenopsis [26]. However, no studies have reported the application of these antimitotic agents for in vitro polyploids induction in C. tigrina.…”

Section: Introductionmentioning

confidence: 99%

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Chromosome doubling in Cattleya tigrina A. Rich

et al. 2019

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Chromosome doubling induction in orchids may benefit their production for resulting in flowers of higher commercial value, larger size and higher content of substances that intensify the color and fragrance when compared with diploid orchids. This work aimed to induce and confirm artificial polyploidization, using flow cytometry and stomatal analysis. Explants were treated with colchicine at concentrations of 0, 2.5, 7.5, and 12.5 mM, for 24 and 48 hours and with oryzalin, at concentrations of 0, 10, 30, and 50 μM, for three and six days. For the flow cytometric analysis, a sample of leaf tissue was removed from each plant, crushed to release the nuclei and stained with propidium iodide. In addition to flow cytometry, the ploidy of the antimitotic treated plants was evaluated by stomata analysis. Young leaves were used where the density, functionality and stomatal index were evaluated. Colchicine provided induction of satisfactory polyploidy in C. tigrina at all concentrations and times of exposure, obtaining a greater number of polyploid individuals in the concentration of 12.5 mM for 48 hours. Oryzalin did not induce chromosome duplication at the tested concentrations.

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Section: Resultsmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Chromosome doubling in Cattleya tigrina A. Rich

et al. 2019

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“…Latado et al (2007) have found that colchicine was toxic to explants of orange 'Pera-de-abril' and tangor 'Murcott'. They observed symptoms of toxicity and mortality, including of explants was relatively low after exposure to 5.0 mM colchicine for 48 or 24 h. Unemoto et al (2009) have stated that the duration of exposure to colchicine is an important factor that affects both the survival and the vegetative development of the plants.…”

Section: Resultsmentioning

confidence: 99%

The effects of colchicine on banana stem apex

Rodrigues

Soares

Brant

et al. 2013

RCA

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The cultivation of traditional varieties of banana in Brazil has suffered from major pests and diseases. The breeding of new strains of banana based on the duplication of chromosomes via treatment with antimitotic agents has been proposed as a way of introducing disease resistance into the hybrids produced by banana breeding programs. The aim of this study was to evaluate the action of colchicine in the apex of diploid banana using an antimitotic at different concentrations and exposure times. Apexes of diploid banana 1318-01 were used as plant material. Colchicine was used at concentrations of 0 (control), 2.5, 5.0, 7.5, 10.0 and 12.5 mM under agitation (20 rpm) for periods of 24 and 48 h. We assessed the survival rate of plants subjected to colchicine. The highest survival rate of the explants (91.7%) in 2.5 mM colchicine was found for 24 h of exposure to the antimitotic, and a survival rate of 50% was found at a concentration of 7.5 mM colchicine for 48 h of exposure. The study showed that the survival of the explants was relatively high after the exposure of diploid 1318-01 to colchicine for a period of 24 h. RESUMO: O plantio de variedades de bananeira tradicionais no Brasil tem sido um fator de vulnerabilidade às principais pragas e doenças que afetam a cultura. Diante disso, estratégias alternativas de melhoramento genético, fundamentadas na duplicação de cromossomos induzida por tratamentos com agente antimitótico, têm sido propostas como forma de introduzir resistência a doenças nos híbridos gerados pelos programas de melhoramento genético da bananeira. O objetivo deste trabalho foi verificar a ação da colchicina em ápices caulinares de bananeira diplóide, utilizando-se diferentes concentrações e tempos de exposição ao antimitótico. Como material vegetal, foram utilizados ápices caulinares de bananeira do diplóide 1318-01. A colchicina foi utilizada nas concentrações de zero (tratamento controle); 2,5; 5,0; 7,5; 10,0; 12,5 mM, em solução, sob agitação (20 rpm), por períodos de 24 e 48 h. Foi avaliada a taxa de sobrevivência de plantas submetidas à colchicina. Verificou-se maior sobrevivência dos explantes (91,7%) em 2,5 mM de colchicina por 24 horas de exposição ao antimitótico e 50% na concentração de 7,5 mM de colchicina por 48 horas de exposição. Conclui-se que a exposição do diplóide 1318-01 à colchicina por um período de 24 h possibilita maior taxa de sobrevivência dos explantes.

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Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), the induction, regeneration or multiplication of shoots, and the preparation and proliferation of plantlets suitable for acclimatization. The initiation of an aseptic culture is closely related to the appropriate selection of an explant source and its preparation, including its (in vivo) pre-treatment if necessary and subsequent disinfection procedures. Care in the choice of explant and the application of an appropriate disinfection protocol can successfully reduce, or eliminate, contamination in in vitro cultures while reducing the negative impact on plant tissues and plantlet regeneration. Many unique aseptic culture procedures for Dendrobium genus have been reported in the literature, very often specific to particular tissues or genotypes, and this review not only highlights the details of such protocols, but also provides practical advice for novice -and even seasoned -orchidologists who wish to research Dendrobium in vitro, although it is cautioned that there is currently no universal aseptic culture procedure that can be applied to all conditions, all explants or all genotypes.

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Sobrevivência e diferenciação de protocormos de Oncidium flexuosum submetidos a tratamento com ácido peracético e colchicina

Cited by 4 publications

References 3 publications

Chromosome doubling in Cattleya tigrina A. Rich

Chromosome doubling in Cattleya tigrina A. Rich

The effects of colchicine on banana stem apex

Tissue disinfection for preparation of Dendrobium in vitro culture

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