Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668

Rahman, Friska Ani; Haniastuti, Tetiana

doi:10.22146/majkedgiind.11325

Cited by 50 publications

(58 citation statements)

References 5 publications

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“…The type of extraction used in this study was maceration extraction [17]. The selection of this extraction method is because it is a simple, easy, and without a heating process, so that the possibility of damage to components of chemical compounds can be minimized [18]. Maceration is also an easy way to do it and uses simple equipment [12].…”

Section: 3qualitative Test Results Of Total Flavonoid Levels In Yomentioning

confidence: 99%

The Analysis of Total Flavonoid Levels In Young Leaves and Old Soursop Leaves (Annona muricata L.) Using UV-Vis Sepctrofotometry Methods

Makuasa

Ningsih

2020

J. Appl. Sci. Eng. Technol. Educ.

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Soursop ( Annona muricata L.) is a type of fruit plant that contains nutrients and is a traditional medicinal ingredient that has multiple properties. This study aims to determine total flavonoid levels in young and old soursop leaf extracts using a UV-Vis spectrophotometry. Extraction of chemical content in young and soursop (Annona muricata L.) leaf extracts was carried out by maceration methos using 96% ehtanol solvent. Determination of total flavonoid levels of sample extracts was carried out through absorpation measurements at a wavelength of 432 nm. The result of the quantitative test were obtained from this study, namely total flavonoid levels in the samples of young soursop leaf extract as much as 410,833 mg/100g, whereas in large soursop leaf extract samples as many as 505,208 mg/100g.

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Section: 3qualitative Test Results Of Total Flavonoid Levels In Yomentioning

confidence: 99%

The Analysis of Total Flavonoid Levels In Young Leaves and Old Soursop Leaves (Annona muricata L.) Using UV-Vis Sepctrofotometry Methods

Makuasa

Ningsih

2020

J. Appl. Sci. Eng. Technol. Educ.

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“…Hydrogen bond formed between phenol group from tannin and carboxyl group (aromatic and aliphatic) from protein, then strong bond between tannin and protein causes inactive enzyme. 17 This was supported by Akiyama and Chung in Rahman FA et al (2017) who stated that tannin can precipitate protein through cell membrane damage because of tannin toxicity and the formation of metal ion bond from tannin which acts in tannin toxicity. Bacteria that grow in aerobic condition need iron for various functions, including reduction from precursor ribonucleotide DNA.…”

Section: Discussionmentioning

confidence: 94%

“…This was caused by tannin in the extract could precipitate protein with tannin complex bonding. 11,[13][14][15][16][17] Tannin is a growth inhibitor which can inhibit the growth of bacteria. The enzyme released by bacteria is basically protein, and protein is precipitated by tannin, thus the enzyme became inactive.…”

Section: Discussionmentioning

confidence: 99%

EFFECT OF KARAMUNTING LEAF EXTRACT (Melastoma malabathricum L.) ON GLUCOSYLTRANSFERASE ENZYME OF Streptococcus mutans

Fajar¹,

Putri²,

Sukmana³

2020

Dentino

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Background: Karamunting leaf extract has secondary metabolite compounds such as tannin, flavonoid, and phenol that have antibacteria potential to be used as an alternative to mouthwash in addition to Chlorhexidine 0.12% to lower the incidence of tooth caries. Caries is caused by several important virulence factors, including Streptococcus mutans Glucosyltransferase (GTF) enzyme. Karamunting leaf extract can inhibit the work of GTF enzyme by precipitating, denaturing protein and damaging cell walls. Inhibition of Streptococcus bacteria enzyme activity will decrease plaque formation, thus decreasing the potential of dental caries. Objective: The purpose of this study was to determine the effect of Karamunting leaf extract and 0,12% Chlorhexidine on Glucosyltransferase enzyme activity of Streptococcus mutans. Methods: This study used true experimental design with post test only with control group design using 7 groups, including Karamunting leaf extract group with 5%, 10%, 15%, 20%, and 25% concentrations to determine the activity of S. mutans GTF enzyme. Results: The results showed that Karamunting leaf extract with 20% concentration was able to decrease the activity of Glucosyltransferase enzyme in Streptococcus mutans. Conclusion: Karamunting leaf extract affects Streptococcus mutans Glucosyltransferase enzyme activity. Keywords: Chlorhexidine 0,12%, Glucosiltransferase, Melastoma malabathricum L., Streptococcus mutans.

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“…Kandungan senyawa bioaktif suatu tumbuhan mengalami perbedaan di-sebabkan juga oleh beberapa faktor. Menurut Rahman et al, (2017) pengaruh lingkungan tempat tumbuh tanaman yaitu iklim, kualitas tanah, dan mutu air dapat mempengaruhi kualitas dan kuantitas metabolit sekunder. Perbedaan habitat tanaman berpengaruh terhadap kandungan fitokimia pada tanaman tersebut.…”

Section: Hasil Dan Pembahasanunclassified

Pemanfaatan Ekstrak Daun Kersen (Muntingia calabura L) untuk Menghambat Pertumbuhan Bakteri Edwardsiella tarda

Zebua

Syawal

Lukistyowati

2019

Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan

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Penelitian telah dilaksanakan pada bulan Juli sampai dengan Oktober 2018. Penelitian ini bertujuan mengetahui bahan bioaktif yang terkandung di dalam ekstrak daun kersen (Muntingia calabura L), sensitivitas ekstrak daun kersen menghambat pertumbuhan bakteri Edwardsiella tarda, dosis Minimum Inhibitory Concentration (MIC) ekstrak daun kersen yang dapat menghambat pertumbuhan bakteri E. tarda dan Lethal dosis50ekstrak daun kersen terhadap ikan lele dumbo (Clarias gariepinus) berukuran 7-9 cm.Daun kersen diekstraksi menggunakan metode maserasi dengan pelarut metanol.Dosis ekstrak daun kersen yang digunakan adalah 10000 ppm, 9000 ppm, 8000 ppm, 7000 ppm, 6000 ppm, 5000 ppm, 4000 ppm, 3000 ppm, 2000 ppm, 1000 ppm dan Novobiocin sebagai kontrol. Hasil uji fitokimia ekstrak daun kersen adalah terpenoid/steroid,flavonoid, fenolik, dan saponin. Hasil uji kromatografi lapis tipis dengan nilai Rf terpenoid/steroid 0,88 cm, flavonoid 0,6 cm, dan fenolik 0,6 cm. Ekstrak daun kersen mampu menghambat pertumbuhan E. tarda hingga dosis 700 ppm dengan rata-rata zona hambat 6,14 mm, Minimum Inhibitory Concentration (MIC) ekstrak daun kersen adalah 700 ppm dengan rerata jumlah koloni 261,66 CFU/mL. Hasil uji toksisitas LD50 ekstrak daun kersen terhadap ikan lele dumbo (Clarias gariepinus) dengan metode perendaman adalah pada dosis 720,06 ppm.Kata kunci : Ekstrak Daun Kersen, Edwardsiella tarda, antibakteri, LD50

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Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668

Cited by 50 publications

References 5 publications

The Analysis of Total Flavonoid Levels In Young Leaves and Old Soursop Leaves (Annona muricata L.) Using UV-Vis Sepctrofotometry Methods

The Analysis of Total Flavonoid Levels In Young Leaves and Old Soursop Leaves (Annona muricata L.) Using UV-Vis Sepctrofotometry Methods

EFFECT OF KARAMUNTING LEAF EXTRACT (Melastoma malabathricum L.) ON GLUCOSYLTRANSFERASE ENZYME OF Streptococcus mutans

Pemanfaatan Ekstrak Daun Kersen (Muntingia calabura L) untuk Menghambat Pertumbuhan Bakteri Edwardsiella tarda

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