Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.
Background: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity.Keywords: Antioxidants, Chitosan, Haruan fish scalesABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity. Keywords: Antioxidants, Chitosan, Haruan fish scales.
Background: Karamunting leaf extract has secondary metabolite compounds such as tannin, flavonoid, and phenol that have antibacteria potential to be used as an alternative to mouthwash in addition to Chlorhexidine 0.12% to lower the incidence of tooth caries. Caries is caused by several important virulence factors, including Streptococcus mutans Glucosyltransferase (GTF) enzyme. Karamunting leaf extract can inhibit the work of GTF enzyme by precipitating, denaturing protein and damaging cell walls. Inhibition of Streptococcus bacteria enzyme activity will decrease plaque formation, thus decreasing the potential of dental caries. Objective: The purpose of this study was to determine the effect of Karamunting leaf extract and 0,12% Chlorhexidine on Glucosyltransferase enzyme activity of Streptococcus mutans. Methods: This study used true experimental design with post test only with control group design using 7 groups, including Karamunting leaf extract group with 5%, 10%, 15%, 20%, and 25% concentrations to determine the activity of S. mutans GTF enzyme. Results: The results showed that Karamunting leaf extract with 20% concentration was able to decrease the activity of Glucosyltransferase enzyme in Streptococcus mutans. Conclusion: Karamunting leaf extract affects Streptococcus mutans Glucosyltransferase enzyme activity. Keywords: Chlorhexidine 0,12%, Glucosiltransferase, Melastoma malabathricum L., Streptococcus mutans.
212, 140, 81, 65, 61, 48, 45, 44, 40, 39, 33 , 25,23,19,17,12, and 11 kDa. On the other hand, results of the protein profile analysis of S. sanguis biofilms using 12% SDS-PAGE showed that there were 15 bands, each of which molecular weight was 130, 85, 65, 61, 48, 46, 40, 37, 29, 25,23,21,17,15, and 12 kDa. And, results
Background: Periodontal disease is one of the dental and oral diseases that is often found in people in Indonesia. RISKESDAS (2018) stated that the prevalence of people who have dental and oral health problems in Indonesia is 57.6%. The prevalence of oral and dental problems in South Kalimantan is 60%. Periodontal disease is damage to the supporting tissues of the teeth caused by specific microorganisms such as Porphyromonas gingivalis which are gram negative bacteria. The growth of these bacteria can be inhibited and killed by chitosan from haruan fish scales. Chitosan is a natural product of chitin, chitosan is the basic ingredient of fish scales. Chitosan has advantages and more safety levels, because it has an active group that will bind to microbes so chitosan can inhibit and kill microbial growth. Objective: to analyze the antibacterial activity of chitosan scales (Channa striata) on the growth of Porphyromonas gingivalis. Methods: This research used true experimental with randomized pre and post test with only control group design with eight treatments. Chitosan of haruan fish scales with liquid dilution method to obtain MIC and solid dilution test to obtain MBC. Results: The results showed that Minimum Inhibitory Concentration (MIC) of chitosan haruan scales was found at a concentration of 2.5% characterized by a decrease in absorbance value of 0.092 and Minimum Bactericidal Concentration found at a concentration of 20% characterized by the absence of Porphyromonas gingivalis. Conclusion: There is an inhibitory activity and a bactericidal activity from chitosan of haruan fish scales on Porphyromonas gingivalis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.