Skiving stacked sheets of paper into test paper for rapid and multiplexed assay

Yang, Ming; Zhang, Wei; Yang, Junchuan; Hu, Binfeng; Cao, Fengjing; Zheng, Wenshu; Chen, Yiping; Jiang, Xingyu

doi:10.1126/sciadv.aao4862

Cited by 76 publications

(54 citation statements)

References 36 publications

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“…As a result, during the movement of the liquid front, the majority of conjugates passed outside the binding zone and were, therefore, lost. Furthermore, the previously described integration of the parallel flows of reagents of different specificities was accomplished with a significant increase in the complexity of the test strip, through additional modifications of the working membrane or the inclusion of additional components in the test strip [17][18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Development of a Lateral Flow Highway: Ultra-Rapid Multitracking Immunosensor for Cardiac Markers

Byzova

Vengerov

Voloshchuk

et al. 2019

Sensors

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The integration of several controlled parameters within a single test system is experiencing increased demand. However, multiplexed test systems typically require complex manufacturing. Here, we describe a multiplexed immunochromatographic assay that incorporates a conventional nitrocellulose membrane, which is used together with microspot printing, to construct adjacent microfluidic "tracks" for multiplexed detection. The 1 mm distance between tracks allows for the detection of up to four different analytes. The following reagents are applied in separate zones: (a) gold nanoparticle conjugates with antibodies against each analyte, (b) other antibodies against each analyte, and (c) antispecies antibodies. The immersion of the test strip in the sample initiates the lateral flow, during which reagents of different specificities move along their tracks without track erosion or reagent mixing. An essential advantage of the proposed assay is its extreme rapidity (1-1.5 min compared with 10 min for common test strips). This assay format was applied to the detection of cardiac and inflammatory markers (myoglobin, D-dimer, and C-reactive protein) in human blood, and was characterized by high reproducibility (8%-15% coefficient of variation) with stored working ranges of conventional tests. The universal character of the proposed approach will facilitate its use for various analytes.

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Section: Discussionmentioning

confidence: 99%

Development of a Lateral Flow Highway: Ultra-Rapid Multitracking Immunosensor for Cardiac Markers

Byzova

Vengerov

Voloshchuk

et al. 2019

Sensors

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Section: Materials For Microfluidic Devicesmentioning

confidence: 99%

“…Due to the flexibility and capillary‐based wettability, paper‐based microfluidics technology has great potential for bioassay‐based personalized healthcare. A paper‐based barcode chip has been developed for multiplexed immunoassays by a rapid mass manufacturing method (Figure C) . Compared with conventional lateral flow immunoassay, this paper‐based barcode assay system can provide simultaneous detection of multiplex targets that allow automated readout by a barcode scanner.…”

Section: Materials For Microfluidic Devicesmentioning

confidence: 99%

Microfluidics‐Based Biomaterials and Biodevices

et al. 2018

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The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adma.201805033.The rapid development of microfluidics technology has promoted new innovations in materials science, particularly by interacting with biological systems, based on precise manipulation of fluids and cells within microscale confinements. This article reviews the latest advances in microfluidics-based biomaterials and biodevices, highlighting some burgeoning areas such as functional biomaterials, cell manipulations, and flexible biodevices. These areas are interconnected not only in their basic principles, in that they all employ microfluidics to control the makeup and morphology of materials, but also unify at the ultimate goals in human healthcare. The challenges and future development trends in biological application are also presented. Microfluidics

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“…[53,54] Paper stacking was demonstrated as a novel approach for assay implementation. [55] Paper sheets, pre-incubated with biologic reagents, were skived into multiple test sheets facilitating mass device manufacture for multiplex applications. The width of a single paper sheet formed each reaction site, with multiple sheets assembled to implement multiplex barcode assays.…”

Section: Cell Targetsmentioning

confidence: 99%