The integration of several controlled parameters within a single test system is experiencing increased demand. However, multiplexed test systems typically require complex manufacturing. Here, we describe a multiplexed immunochromatographic assay that incorporates a conventional nitrocellulose membrane, which is used together with microspot printing, to construct adjacent microfluidic "tracks" for multiplexed detection. The 1 mm distance between tracks allows for the detection of up to four different analytes. The following reagents are applied in separate zones: (a) gold nanoparticle conjugates with antibodies against each analyte, (b) other antibodies against each analyte, and (c) antispecies antibodies. The immersion of the test strip in the sample initiates the lateral flow, during which reagents of different specificities move along their tracks without track erosion or reagent mixing. An essential advantage of the proposed assay is its extreme rapidity (1-1.5 min compared with 10 min for common test strips). This assay format was applied to the detection of cardiac and inflammatory markers (myoglobin, D-dimer, and C-reactive protein) in human blood, and was characterized by high reproducibility (8%-15% coefficient of variation) with stored working ranges of conventional tests. The universal character of the proposed approach will facilitate its use for various analytes.
Complex problems of three-dimensional elasticity theory for thick elastic plates with arbitrary geometry of their lateral surface are solved. Analytical dependencies of the components of the elastic dynamic displacements’ field of the plate’s inner points from the boundary-surface external-dynamic disturbing factors, defined by continuous functions or their values’ vectors, are constructed. It is assumed, that these disturbances have a classically defined powerful character, or are specified by a certain number of differential transformations of the field’s components of the plate’s dynamic displacement points. The absence of quantitative and qualitative restrictions on the determined transformations of the initial-boundary problems of the considered plates’ dynamics makes it incorrect and unsolvable by methods of classical and computational mathematics. The methodology of root-mean square mathematical modeling of discretely and continuously specified observations for the initial-boundary plate’s condition by the system of modeling functions and their values’ vectors is proposed in the paper. Constructed in this way field’s components of spatial-dynamic displacements of the plate’s points, precisely satisfying classical Lyame equation, with the available information on its initial-boundary condition, are agreed according to the root-mean square criterion. The problem of the obtained solutions’ ambiguity is investigated, their accuracy evaluation in accordance with the information on the external- dynamic condition of the investigated plate is conducted. The plate’s dynamics in the particular mode, for cases of information lack on external- dynamic influences on it and under the conditions of its geometric background according to spatial coordinates. The computer realization of the obtained mathematical results is engineeringly simple and can be easily implemented with the help of well-known methods of computational mathematics.
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