Site-Specific Immobilization of Single-Walled Carbon Nanotubes onto Single and One-Dimensional DNA Origami

Abstract

Development of a simple and efficient methodology to control the placement, spacing, and alignment of single-walled carbon nanotubes (SWCNTs) is essential for nanotechnology device application. Building on the growing understanding that the strong π-π interaction between the bases of single-stranded DNA (ssDNA) and CNTs is sufficient not only to drive CNT solubility in water but also to stabilize individual nanotubes against clustering in aqueous solution, a new motif for functionalizing DNA origami (DO) with CNTs is demonstrated. CNTs solubilized via wrapping with ssDNA react with DO constructs displaying linear arrays of ssDNA, leading to immobilization of the CNTs onto the DO scaffold. This study demonstrates the immobilization of ssDNA-wrapped CNTs at specific positions on single DO constructs. Furthermore, multiple DO constructs assembled into extended one-dimensional arrays have been used to successfully align pairs of CNTs exceeding 500 nm in length in a parallel orientation. This result provides a simplified, alternative approach to immobilization of CNTs with programmed spacing and orientation.

Investigation of the self‐assembly process for discrete and polymerized bivalve DNA origami structures

“…The DNA origami structures can be robustly produced utilizing the self‐assembly formation of DNA double helices during an annealing procedure to fold a long, single‐stranded DNA (scaffold strand) with the help of a group of short, single‐stranded DNAs (staple strands) into a discrete nanoscale object. Diverse nanoscale materials such as metal particles , carbon nanotubes (CNTs), and quantum dots have been selectively arranged at specific positions of a DNA origami template with spatial addressability of a few nanometers. These DNA origami templates have been used for a wide range of applications , such as optical or chemical sensing.…”

Hybrid material of structural DNA with inorganic compound: synthesis, applications, and perspective

“…Controlled assembly of carbon nanotubes has been also executed [101][102][103]. Inspired by the accurate positioning of carbon nanotubes on a two-dimensional DNA origami, it was possible to design a universal set of carbon nanotube circuit [104].…”

Fault Tolerant Design and Analysis of Carbon Nanotube Circuits Affixed on DNA Origami Tiles

“…In this way, highly complex patterns can, and have been, experimentally achieved, with an unprecedented high level of accuracy and reliability. Moreover, several authors have announced the formation of DNA origami tiles capable of further assembly into larger, fully addressable, 1D and 2D template lattices [10], [11], [12]. Such scaffolds make possible the construction of highly complex structures on top of them [6], [10], [13], [14], prospectively including nanocircuits.…”

“…After the introduction of the DNA origami technique, researchers concentrated over the integration of elemental nanoelectric components on top of these individualized structures [1], [5]. Current developments in the field are particularly achieving a high level of accuracy [10], [13]. Two recent surveying presentations on the topic of DNAassisted nanoelectric circuitry are [6], [8].…”