Investigation of the self‐assembly process for discrete and polymerized bivalve DNA origami structures

Ma, Zhipeng; Park, Seongsu; Yamashita, Naoki; Hirai, Yoshikazu; Tsuchiya, Toshiyuki; Tabata, Osamu

doi:10.1002/tee.22249

Cited by 1 publication

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References 24 publications

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“…The collapse and reduced thickness of DNA origami nanostructures in air were also observed previously in the single-layered and multilayered DNA origami nanostructures. 23,24) The thickness of the single layer of DNA origami in zigzag lattice is measured in air to be about 1.5 nm, 23,24) which is in good agreement with the current measurement of DNA origami nanotubes. The measured diameters also show a clear dependence on the crossover design along the tube axis.…”

Section: Structural Characterization Of Dna Origami Nanotubessupporting

confidence: 84%

Tuning porosity and radial mechanical properties of DNA origami nanotubes via crossover design

Kawai

Hirai

et al. 2017

Jpn. J. Appl. Phys.

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DNA origami nanotubes are utilized as structural platforms for the fabrication of various micro/nanosystems for drug delivery, optical or biological sensing, and even nanoscale robots. Their radial structural and mechanical properties, which play a crucial role in the effective use of micro/nanosystems, have not been fully studied. In particular, the effects of crossovers, which are basic structures for rationally assembling double-stranded DNA (dsDNA) helices into a nanotube configuration, have not yet been characterized experimentally. To investigate the effects of crossovers on the porosity and the radial mechanical properties of DNA origami nanotubes, we fabricated a DNA origami nanotube with varied crossover designs along the nanotube axis. The radial geometry of the DNA origami nanotube is experimentally characterized by both atomic force microscopy (AFM) and electron cryomicroscopy (cryo-EM). Moreover, the radial mechanical properties of the DNA origami nanotube including the radial modulus are directly measured by force–distance-based AFM. These measurements reveal that the porosity and the radial modulus of DNA origami nanotubes can be tuned by adjusting the crossover design, which enables the optimal design and construction of DNA origami nanostructures for various applications.

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