Single hair analysis: Validation of a screening method for over 150 analytes and application on documented single‐dose cases

Wiedfeld, Christopher; Skopp, Gisela; Mußhoff, Frank

doi:10.1002/dta.2997

Cited by 15 publications

(32 citation statements)

References 24 publications

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“…The characteristic profile of three consecutive concentration peaks obtained for each of the three substances detected in all analyzed hairs reveals a repeated intake. Due to the distinct separation of the peaks and an approximate distance of at least 5 mm between their maxima, a contribution to the peak pattern due to different routes of incorporation appears unlikely 8,12,13 . Therefore, each peak can be assigned to a separate and probably single intake of the respective substance; in total, peaks indicate toward at least three time points of administration.…”

Section: Discussionmentioning

confidence: 99%

“…The detailed segmentation and extraction procedure has been described in our previous publication also covering validation data and parameters of the applied liquid chromatography–tandem mass spectrometry method (LC‐MS/MS) 8 …”

Section: Methodsmentioning

confidence: 99%

“…To gain further information on the frequency and time point of drug administration, single hair analysis appears to be a promising tool and has aroused interest by various working groups in the past years 9–11 . We have recently presented a fully validated multi‐analyte screening method (156 analytes) for single hair analysis that had been applied successfully to single‐dose cases 8 . This method and resulting knowledge derived from interpreting the single‐dose profiles were applied to a routine DFSA case of a child.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Application of single hair analysis reveals multiple administration of a drug mixture in a case of drug‐facilitated sexual assault of a child

Wiedfeld

Skopp

Kuepper

et al. 2021

Drug Testing and Analysis

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of single hair analysis reveals multiple administration of a drug mixture in a case of drug‐facilitated sexual assault of a child

Wiedfeld

Skopp

Kuepper

et al. 2021

Drug Testing and Analysis

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“…LA-ICP-MS technology was used to investigate the longitudinal distribution of arsenic in a single hair in situ , and a mathematical model was established to predict arsenic entry time based on the retention position of arsenic in the hair and the growth rate of the hair [ 49 ] ( Figure 4 ). A method for manually segmenting single hairs in 2-mm sections and screening for 156 analytes using LC-MS/MS was developed, and applied in 15 single dose cases by analyzing ten hairs each [ 50 ]. Many substances including mirtazapine could be detected in the presented cases, whereas detection of benzodiazepines and low dosed opioids remains challenging.…”

Section: Further Development Of Hair Analysismentioning

confidence: 99%

Current status of hair analysis in forensic toxicology in China

Yan

Xiang

Shen

2021

Forensic Sciences Research

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Hair analysis has been mainly used to document drug use history in abusers, drug-facilitated crime cases, doping control analysis and postmortem toxicology in the fields of forensic toxicology, clinical toxicology, and doping control. Hair analysis has also gained more attention in the last 30 years in China. Relevant technology has been promoted as more research has appeared concerning hair analysis, and consensus has been sought among forensic toxicologists regarding aspects such as hair decontamination treatment, detection of abused substances in hair, segmental hair analysis and interpretation of analytical results. However, there are still some limitations in the estimation of drug intake time and frequency by segmental hair analysis due to the different growth cycles evident within a bundle of hairs, the drug incorporation mechanism and sampling errors. Microsampling and imaging mass spectrometry (iMS) technology based on a single hair may be a good choice to estimate drug intake time more accurately. Analysis of hair root samples may also be used to document acute poisoning in postmortem toxicology, and the analysis of the hair shaft can document long-term use of drugs depending on the length of the hair being evaluated. Current application of hair analysis in China Confirmation of drug intake and investigation of drug use historyCompared with biological samples such as blood and urine, hair analysis has the advantages of stable presence of exogenous drugs, extended detection window, and the ability to reflect longterm drug abuse history. In 1999, meperidine was detected in

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“…Wiedfeld et al recently developed the method to analyze over 150 analytes using 2-mm segmentation of a single hair strand and LC-MS/MS 29. They estimated the mean growth rate of a hair strand by comparing the hair positions of a targeted drug between hair strands collected on two different days.…”

mentioning

confidence: 99%

Development of an improved method to estimate the days of continuous drug ingestion, based on the micro‐segmental hair analysis

Kuwayama

Miyaguchi

Kanamori

et al. 2021

Drug Testing and Analysis

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To prove drug-related crimes, it is important to estimate the date on which a specific drug was ingested. Previously, we developed a method, "micro-segmental hair analysis," to estimate the day of ingestion of a single-dose drug by segmenting a hair strand into 0.4-mm segments, which correspond to daily hair growth. In this study, the method was improved to estimate the days of continuous drug ingestion. The subjects ingested four hay-fever medicines (fexofenadine, epinastine, cetirizine, and loratadine) continuously (1-18 days) and chlorpheniramine as a single dose at intervals of several weeks as an internal temporal marker (ITM). The hair strands of the subjects were collected and subjected to a micro-segmental analysis. The distribution curves of each hay-fever medicine in a hair strand had broad peaks reflecting the number of days of drug ingestion. The positions on the curves corresponding to the first and final ingestion days of hay-fever medicines were identified using the ITM.The positions were near the hair segments on both ends of full width at half maximum (W 2 ) of the broad peak. When the first and final days of continuous ingestion were estimated using W 2 , independent of peak shape, the absolute average error from the actual ingestion days was approximately 2 days. Overall, we established a method to estimate the days of both single-dose and continuous drug ingestions.Furthermore, the method would be useful to investigate drug ingestion history in various scenes such as drug-related crimes and therapeutic drug monitoring.

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Single hair analysis: Validation of a screening method for over 150 analytes and application on documented single‐dose cases

Cited by 15 publications

References 24 publications

Application of single hair analysis reveals multiple administration of a drug mixture in a case of drug‐facilitated sexual assault of a child

Application of single hair analysis reveals multiple administration of a drug mixture in a case of drug‐facilitated sexual assault of a child

Current status of hair analysis in forensic toxicology in China

Development of an improved method to estimate the days of continuous drug ingestion, based on the micro‐segmental hair analysis

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