Simultaneous Estimation of Glimepiride, Rosiglitazone and Pioglitazone Hydrochloride in the Pharmaceutical Dosage Form

Havaldar, Freddy H.; Vairal, Dharmendra L.

doi:10.1155/2010/262810

Cited by 15 publications

(7 citation statements)

References 7 publications

(7 reference statements)

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“…Analytical methods for the determination of PIO in pharmaceutical dosages forms using HPLC [35] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] , [67] are shown in Table 3 , while Fig. 5 shows the best HPLC methods for the analysis of PIO.…”

Section: Methodsmentioning

confidence: 99%

Pioglitazone: A review of analytical methods

Satheeshkumar

Saladi

Srinivas

2014

Journal of Pharmaceutical Analysis

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Pioglitazone is an oral anti-hyperglycemic agent. It is used for the treatment of diabetes mellitus type 2. It selectively stimulates nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-gamma). It was the tenth-best-selling drug in the U.S. in 2008. This article examines published analytical methods reported so far in the literature for the determination of pioglitazone in biological samples and pharmaceutical formulations. They include various techniques like electrochemical methods, spectrophotometry, capillary electrophoresis, high-performance liquid chromatography, liquid chromatography–electrospray ionization-tandem mass spectrometry and high-performance thin layer chromatography.

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Section: Methodsmentioning

confidence: 99%

Pioglitazone: A review of analytical methods

Satheeshkumar

Saladi

Srinivas

2014

Journal of Pharmaceutical Analysis

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“…There are some HPLC methods [17][18][19][20][21][22][23][24] reported in the literature, but these methods are developed only for individual analysis of Pioglitazone and Rosiglitazone in bulk and formulation studies. The developed HPLC method was utilized for the estimation of the combined drugs by in vitro method.…”

Section: Introductionmentioning

confidence: 99%

New Validated Method for the Estimation of Pioglitazone and Rosiglitazone Using RP-HPLC

Rafi¹,

Rambabu²

2021

JPRI

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New, simple and economical high pressure liquid chromatography method has been developed for the simultaneous quantification of Pioglitazone and Rosiglitazone.By using Waters HPLC e-2695 quaternary pump with a PDA detector of 2998 instrument the chromatographic separation of Pioglitazone and Rosiglitazone was achieved on the column of Inertsil ODS (150x4.6mm, 3.5 µ) using an isocratic elution with a buffer containing 0.1percentformic acid and acetonitrile at a rate of 30:70 as a mobile phase with a flow rate of 1 ml/min at ambient temperature. A detector wavelength of 261 nm utilizing the PDA detector were given in the instrumental settings. The linearity was studied between the concentration range of 3-45 µg/ml of Pioglitazone and 1-15 µg/ml of Rosiglitazone were injected.The plotted calibration curves were linear with a regression coefficient of R2> 0.999, indicates that the linearity was with in the limit. As a part of method validation the parameters like specificity, linearity, accuracy, ruggedness, robustness were determined and the results were found to be within the allowable limit. The method developed was found to be applicable to routine analysis and to be used for the measurement of both active pharmaceutical ingredients (i.e, Pioglitazone and Rosiglitazone).Validation of the proposed method was carried out according to an International Conference on Harmonization (ICH) guidelines. Since, there is no HPLC method reported in the literature for the estimation of Pioglitazone and Rosiglitazone, there is a need to develop quantitative methods under different conditions to achieve improvement in specificity, selecivity etc.

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“…Although several analytical methods were reported for analysis of each component individually, few methods were reported for the simultaneous determination of MET and PIO in combination dosage forms. These methods include; spectroscopic and chemometric methods [7][8], thin layer chromatographic (TLC) methods [9] and high performance liquid chromatographic (HPLC) method [10][11][12][13][14][15][16]. On the other hand, the only report for their simultaneous determination in plasma sample was using LC-MS technique [17].…”

Section: Introductionmentioning

confidence: 99%