An efficient hydrophilic interaction liquid chromatographic method for the simultaneous determination of metformin and pioglitazone using high-purity silica column

For the determination of Metformin in human serum, a facile colorimetric and spectrophotometric sensor was designed based on citrate-capped gold nanoparticles (citrate-GNPs). In this probe, the addition of Metformin to GNP solution generates a naked-eye color change resulting from the aggregation of GNPs. Study of this color conversion and quantity analysis of analyte is operated by spectrophotometric instruments. The three factors pH, time, and GNP ratio were selected to examine their effects on sensing results and their values optimization. The optimization of parameters was done by means of central composite design and one-at-a-time methods. The sensing results proved the highly selective and sensitive performance of the sensor for Metformin in a linear range of 6.25–133.3 ppm with a detection limit of 1.79 ppm. The relative standard deviation (RSD) of the reported method is 2.53%.

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“…However, these methods have low sensitivity and a time-consuming sample pretreatment procedure is required. 1,9−11…”

Section: Introductionmentioning

confidence: 99%

A Facile Colorimetric and Spectrophotometric Method for Sensitive Determination of Metformin in Human Serum Based on Citrate-Capped Gold Nanoparticles: Central Composite Design Optimization

Shahbazi

Zare‐Dorabei

2019

ACS Omega

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“…Following a thorough study of the literature, several methods for analyzing MET alone in various samples (plasma, urine, liver, brain, kidney, and muscles) were reported, including HPLC [14][15][16][17][18][19], HILIC [20], LC/MS/MS [21][22][23][24], HILIC/MS [25], and GC/MS [26]. MET in conjunction with various medications in various plasma samples was also estimated by HPLC [27][28][29][30][31][32], LC/MS/MS [33][34][35][36][37][38][39][40], and HILIC/MS [41,42] methods.…”

Section: Introductionmentioning

confidence: 99%

Development and Greenness Assessment of HPLC Method for Studying the Pharmacokinetics of Co-Administered Metformin and Papaya Extract

et al. 2022

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Foods with medical value have been proven to be beneficial, and they are extensively employed since they integrate two essential elements: food and medication. Accordingly, diabetic patients can benefit from papaya because the fruit is low in sugar and high in antioxidants. An RP-HPLC method was designed for studying the pharmacokinetics of metformin (MET) when concurrently administered with papaya extract. A mobile phase of 0.5 mM of KH2PO4 solution and methanol (65:35, v/v), pH = 5 ± 0.2 using aqueous phosphoric acid and NaOH, and guaifenesin (GUF) were used as an internal standard. To perform non-compartmental pharmacokinetic analysis, the Pharmacokinetic program (PK Solver) was used. The method’s greenness was analyzed using two tools: the Analytical GREEnness calculator and the RGB additive color model. Taking papaya with MET improved the rate of absorption substantially (time for reaching maximum concentration (Tmax) significantly decreased by 75% while maximum plasma concentration (Cmax) increased by 7.33%). The extent of absorption reduced by 22.90%. Furthermore, the amount of medication distributed increased (30.83 L for MET concurrently used with papaya extract versus 24.25 L for MET used alone) and the clearance rate rose by roughly 13.50%. The results of the greenness assessment indicated that the method is environmentally friendly. Taking papaya with MET changed the pharmacokinetics of the drug dramatically. Hence, this combination will be particularly effective in maintaining quick blood glucose control.

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“…Analysis of PIO was achieved using different methods and reported in the literature [ 19 ]. The recently developed techniques included spectrophotometry [ 20 , 21 , 22 ], chromatography including TLC [ 23 ] and HPLC [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Induced mathematical filtration as an innovative strategy for discrimination and estimation of glycemic control drugs in fixed dose combination

Lotfy

Mohamed

2020

Heliyon

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An innovative strategy was developed for the estimation of a fixed dose combination containing Alogliptin (ALO) and pioglitazone (PIO) using induced concept for resolving the overlapped spectra, lacking isoabsorptive point. This strategy is based on coupling factors as numerical values or ratios as spectrum form with the recorded signals leading to induced mathematical filtration of the drug of interest and complete elimination of the interfering one in the combination without prior physical separation. The calculated factors were factor of equality in induced dual wavelength (IDW) or absorptivity factor in induced concentration subtraction method (ICS) while absorptivity ratio spectrum for induced amplitude modulation method (IAM). The calibration curves displayed linearity within 1.0–16.0 μg/mL for ALO and 2.0–22.0 μg/mL for PIO with good correlation coefficients. The induced methods specificity was also assured through the assaying different synthetic mixtures prepared to contain the two drugs in ratios approaching the ratio actually found in the marketed dosage form. The methods were applicable and suitable for estimating ALO and PIO in both bulk form and their fixed dose combination. Induced methods have been extensively validated in accordance with ICH guidelines and results demonstrated the accuracy and reproducibility in comparison to the reported method.

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An efficient hydrophilic interaction liquid chromatographic method for the simultaneous determination of metformin and pioglitazone using high-purity silica column

Cited by 22 publications

References 18 publications

A Facile Colorimetric and Spectrophotometric Method for Sensitive Determination of Metformin in Human Serum Based on Citrate-Capped Gold Nanoparticles: Central Composite Design Optimization

A Facile Colorimetric and Spectrophotometric Method for Sensitive Determination of Metformin in Human Serum Based on Citrate-Capped Gold Nanoparticles: Central Composite Design Optimization

Development and Greenness Assessment of HPLC Method for Studying the Pharmacokinetics of Co-Administered Metformin and Papaya Extract

Induced mathematical filtration as an innovative strategy for discrimination and estimation of glycemic control drugs in fixed dose combination

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