Simultaneous determination of sulfamethoxazole and trimethoprim in microgram quantities from low plasma volume by liquid chromatography–tandem mass spectrometry

Mistri, Hiren N.; Jangid, Arvind G.; Pudage, Ashutosh; Shah, Alay; Shrivastav, Pranav S.

doi:10.1016/j.microc.2009.10.002

Cited by 30 publications

(13 citation statements)

References 26 publications

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“…Several analytical techniques have been reported for determination of SMX in pharmaceutical formulations and biological fluids. Most of these techniques employed separation methods, such as solid phase extraction-liquid chromatography using an on line clean-up column coupled with amperometric detection employing a boron-doped diamond (BDD) electrode (Andrade et al, 2009), HPLC-tandem mass spectrometry (LC-MS/MS) (Mistri et al, 2010) or capillary electrophoresis (Qing-Cui et al, 2008). Other techniques such as differential pulse voltammetry (DPV) (Joseph and Kumar, 2010), square wave voltammetry (SWV) (Souza et al, 2008), micellar electrokinetic capillary chromatography (Injac et al, 2008), partial least square regression method (Givianrad et al, 2013), flow injection system /HPLC (Sabriye et al, 2011) and ratio derivative spectrophotometry (Hajian et al, 2010) have been also used for determination of SMX.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Sulfamethoxazole in Pure and in Pharmaceutical Preparations by Diazotization and Coupling Reaction

Mohammed¹,

Al-Hamdany²,

Abdulkader³

2018

RJS

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A highly sensitive, simple and accurate spectrophotometric method has been developed for quantitative determination of sulfamethoxazole(SMX) in both pure form and pharmaceutical preparations. In this method SMX is diazotized with equimolar of sodium nitrite(NaNO 2) in acid medium of hydrochloric acid to form diazonium ion , which is reacted with 2,4,6-trihydroxybenzoic acid in alkaline medium of NaOH to form a yellow water soluble azo dye that has absorption maximum at 416 nm versus reagent blank. Beer's law is obeyed over the concentration range 0.2-16 µg. ml-1 with an excellent determination coefficient (r 2 = 0.9996) and molar absorptivity 1.84×10 4 l.mol-1 .cm-1. The recoveries are obtained in the range of 97.8-99.8% and the relative standard deviation is better than ±0.23%. The stoichiometry of the resulting azo dye has been also worked out and it is found to be 1:1 SMX: 2,4,6-trihydroxybenzoic acid. This method has been applied successfully for the determination of SMX in pharmaceutical preparations (tablets and oral suspension).

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Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Sulfamethoxazole in Pure and in Pharmaceutical Preparations by Diazotization and Coupling Reaction

Mohammed¹,

Al-Hamdany²,

Abdulkader³

2018

RJS

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“…Literature survey reveals that various techniques have been used for the determination of SMZ, such as spectrophotometry 13, ratio spectra derivative spectrophotometry 14, flow injection spectrophotometry 15, gas chromatography‐mass spectrometry 16, capillary electrophoresis 17, liquid chromatography 18, high performance liquid chromatography (HPLC) using an on‐line clean‐up column 19, high performance thin layer chromatography 20, liquid Chromatography‐mass spectrometry (LC‐MS) 21 and visible and UV spectrophotometry 22. Most of these methods were time consuming, expensive and required tedious extraction and separation steps.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Sensitive Poly‐1,5‐Diaminonaphthalene Modified Sensor for the Determination of Sulfamethoxazole in Biological Samples

Chasta

Goyal

2015

Electroanalysis

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Sulfamethoxazole (SMZ), an antibacterial sulfonamide drug, has been selectively determined using poly‐1,5‐diaminonaphthalene (p‐DAN) modified glassy carbon electrode (GCE). The modified sensor was characterized by field emission scanning electron microscopy (FE‐SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). SMZ showed linear response in the concentration range of 0.5–150 µM by using square wave voltammetry (SWV) and the detection limit was found to be 0.05 nM with sensitivity of 0.085 µA µM−1. The proposed sensor has been successfully employed to determine SMZ in the pharmaceutical tablets and human urine samples.

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“…Various methods have been reported for determination of SMX and TMP in pharmaceutical formulations and biological fluids [3][4][5][6][7][8][9][10][11][12][13][14] . Most of these methods use a separation method such as high-performance liquid chromatography (HPLC) [3][4][5][6][7][8] or capillary electrophoresis 9 . The USP monograph 15 lists a high-performance liquid chromatographic method as the official assay procedure for quality control.…”

Section: Introductionmentioning

confidence: 99%

Net Analyte Signal Standard Additions Method for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Pharmaceutical Formulations and Biological Fluids

Givianrad

Mohagheghian

2012

E-Journal of Chemistry

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The applicability of a novel net analyte signal standard addition method (NASSAM) to the resolving of overlapping spectra corresponding to the sulfamethoxazole and trimethoprim was verified by UV-visible spectrophotometry. The results confirmed that the net analyte signal standard additions method with simultaneous addition of both analytes is suitable for the simultaneous determination of sulfamethoxazole and trimethoprim in aqueous media. Moreover, applying the net analyte signal standard additions method revealed that the two drugs could be determined simultaneously with the concentration ratios of sulfamethoxazole to trimethoprim varying from 1:35 to 60:1 in the mixed samples. In addition, the limits of detections were 0.26 and 0.23 μmol L-1for sulfamethoxazole and trimethoprim, respectively. The proposed method has been effectively applied to the simultaneous determination of sulfamethoxazole and trimethoprim in some synthetic, pharmaceutical formulation and biological fluid samples.

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Simultaneous determination of sulfamethoxazole and trimethoprim in microgram quantities from low plasma volume by liquid chromatography–tandem mass spectrometry

Cited by 30 publications

References 26 publications

Spectrophotometric Determination of Sulfamethoxazole in Pure and in Pharmaceutical Preparations by Diazotization and Coupling Reaction

Spectrophotometric Determination of Sulfamethoxazole in Pure and in Pharmaceutical Preparations by Diazotization and Coupling Reaction

A Simple and Sensitive Poly‐1,5‐Diaminonaphthalene Modified Sensor for the Determination of Sulfamethoxazole in Biological Samples

Net Analyte Signal Standard Additions Method for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Pharmaceutical Formulations and Biological Fluids

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