Net Analyte Signal Standard Additions Method for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Pharmaceutical Formulations and Biological Fluids

Givianrad, Mohammad Hadi; Mohagheghian, M.

doi:10.1155/2012/274396

Cited by 9 publications

(4 citation statements)

References 17 publications

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“…Nowadays, NASSAM procedure has been widely applied alone or in conjugation with various sophisticated analytical methods, including DT of cocrystal forms [5] . Other recent examples include simultaneous determination of sulphadiazine and trimethoprim in bovine milk and veterinary medicines [6] , determination of sulfamethoxazole and trimethoprim in pharmaceutical formulations and biological fluids [7] and antazoline and naphazoline determination with NASSAM and spectrophotometric methods [8] . Simultaneous estimation is the analysis of standards present in multiple combination dosage form at the same time period.…”

Section: Introductionmentioning

confidence: 99%

Application of RP–HPLC method in dissolution testing and statistical evaluation by NASSAM for simultaneous estimation of tertiary combined dosages forms

Yogesh

Sharma

Sarma

et al. 2015

Journal of Pharmaceutical Analysis

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A dissolution method with robust high performance liquid chromatographic (HPLC) analysis for immediate release tablet formulation was developed and validated to meet the requirement as per International Conference on Harmonization (ICH) and United States Food and Drug Administration (USFDA) guidelines. The method involved the use of Agilent ZORBAX Eclipse XDB C18 column, and temperature was maintained at 30 °C. After optimization, the mobile phase was selected as phosphate buffer (KH2PO4, 30 mM) : ACN (60:40, v/v) with pH 3.0, and retention time Rt was found as 3.24, 4.16, and 2.55 min for paracetamol (PCM), chlorpheniramine maleate (CPM) and phenylephrine hydrochloride (PH) respectively at 265 nm and at a flow rate of 1 mL/min. The relative standard deviation (%RSD) for 6 replicate measurements was found to be less than 2%. Furthermore net analyte signal standard addition method (NASSAM) with spectrophotometer was performed for standard and liquid oral suspension. On the basis of selectivity, sensitivity and accuracy analysis, it was confirmed that this novel method could be useful for simultaneous estimation of the given drug combinations. Two-way analysis of variance (ANOVA) was applied for evaluating the statistical difference between the assay results obtained via both NASSAM and RP–HPLC methods and ultimately no significant difference was found between both the methods. All the methods and results were acceptable and confirmed that the method was suitable for intended use.

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Section: Introductionmentioning

confidence: 99%

Application of RP–HPLC method in dissolution testing and statistical evaluation by NASSAM for simultaneous estimation of tertiary combined dosages forms

Yogesh

Sharma

Sarma

et al. 2015

Journal of Pharmaceutical Analysis

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“…Frequently, the formulation products contain a mixture of sulfonamide with other drugs to increases the potential of the product such as sulphamethoxazole and trimethoprim product which is known as cotrimoxazole [1]. Searching the literature showed various instrumental methods for the analysis of sulpha drugs such as, GC [2], HPLC [3][4][5], flow injection [6][7][8], HPTLC [9], micellar electrokinetic capillary chromatography (MEKC) [10], voltammetry [11], solid phase extraction (SPE) [12] and spectrophotometric methods [13][14][15][16]. Some of the reported spectrophotometric methods require long heating times and a lot of data manipulation, which makes some difficult for their application as standard routine methods.…”

Section: Sulphamethoxazolementioning

confidence: 99%

Development Method for Spectrophotometric Analysis of Sulfamethoxazole Using Vanilline Reagent

Mohammed

Sabha

Jabar

2020

AJACR

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This study involves a development method for spectrophotometric analysis of sulfamethoxazole (SMZ) with vanillin reagent in acidic medium. The reaction mechanism was proposed by Schiff's base reaction of the amine group in SMZ with the carbonyl group in vanillin. The spectrum of SMZ complex showed high absorbance at 399.09 nm following the Beer–Lambert law in the concentration levels ranging from 5 to 80 µg.mL-1 with good sensitivity. The stability constant of the product was determined. The values of relative standard deviation (≤ 6.63) and recovery (> 98.73%) indicated a reasonably precise and accurate method with a good limit of detection and quantification. The present method was compared with other spectrophotometric methods in the literature. The procedure of the method showed to be simple since no need for temperature control and solvent extraction. The suggested method was used to determine of SMZ in commercial pharmaceutical drops and compared with the certified method.

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“…According to a literature review, only a few analytical procedures for Sulfamethoxazole analysis have been published. Some spectrophotometric methods [6,10], HPLC [11,18], As For an example, Flow Injection Analysis (FIA) [19] and Micellar Electro Kinetic Capillary Chromatography (MEKC) [20]. The purpose of this study is to develop an improved spectrophotometric approach based on the ion pair reaction with the new reagent EBT for estimating trace concentrations utilizing dispersive liquid-liquid extraction.…”

Section: Introductionmentioning

confidence: 99%

New spectrophotometric determination sulfamethoxazole drug via various analytical methods in pharmaceutical formulation

Jamil,

Raghda S. AlSayed,

Nisreen Kais Abood

2023

J. K. Chem. Sci.

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For the determination of sulfamethaxazole (SMZ) in pure and pharmaceutical formulations, new simple sensitive spectrophotometric methods have been devised. The first technique involved forming an ion pair complex between the basic nitrogen of the SMZ medication and Eriochrome black (EBT) in an acidic buffer solution, and extracting the created complex with chloroform to get a persistent reddish-orange colored dye with a maximum wavelength of 490 nm. Beer's law was followed, and 0.9998 was the correlation coefficient., 0.471×104L. mol-1.cm-1was the molar absorptivity, and the detection limit was 0.177 g/mL. Dispersive liquid-liquid extraction (DLLE) is a second method for estimating a trace amount in an aqueous solution product using an ion pair and a UV-visible spectrophotometer with a maximum wavelength of 490 nm. The concentration range was 0.5-6 g/mL, with a correlation coefficient of 0.9995, 0.889×105 L.mol-1.cm-1, a molar absorptivity of ,the detection limit of 0.013 g/L, and a pre-concentration factor of 25 and an enrichment factor of 18.20. Sulfamethaxazole in pharmaceutical formulations was successfully determined using the provided methodology

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Net Analyte Signal Standard Additions Method for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Pharmaceutical Formulations and Biological Fluids

Cited by 9 publications

References 17 publications

Application of RP–HPLC method in dissolution testing and statistical evaluation by NASSAM for simultaneous estimation of tertiary combined dosages forms

Application of RP–HPLC method in dissolution testing and statistical evaluation by NASSAM for simultaneous estimation of tertiary combined dosages forms

Development Method for Spectrophotometric Analysis of Sulfamethoxazole Using Vanilline Reagent

New spectrophotometric determination sulfamethoxazole drug via various analytical methods in pharmaceutical formulation

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