Simultaneous Determination of Pyridoxine Hydrochloride and Doxylamine Succinate in Tablets by HPTLC

Simultaneous determination of clobutinol hydrochloride and doxylamine succinate from syrups by RP HPLC using a new stationary phase containing embedded urea polar groups

“…The organic solvents acetonitrile and methanol have different effects on the solvation of the analytes and therefore they also affect the symmetry and selectivity of the peaks (Argekar, Sawant, 2005). The best chromatographic condition was obtained with a mobile phase containing a mixture of 27% acetonitrile, 2% methanol and 71% buffer; this condition provided the best resolution of CLO and DOX.…”

“…It is used for treating nausea during pregnancy and it is present in over the counter cold and sedative pharmaceuticals (Sthal, 1998). Some chromatographic methods have been proposed for its determination at trace levels (Argekar, Sawant, 1999;Argekar, Sawant, 2005), mass spectrometry (Hansen et al, 1985), capillary electrophoresis (Tang et al, 2000), UV spectrometry in a micellar medium (Monferrer-Pons et al, 1996) and Fourier transform infrared spectroscopy (FTIR) (Ventura-Gayete et al, 2006) have also been used. Simultaneous determination of DOX in combination with carbinoxamine maleate by spectrophotometry (MonferrerPons at al., 1996), in combination with dextromethorphan hydrobromide by HPTLC (Indrayanto, 1996), in combination with pseudoephedrine hydrochloride and dextromethorphan hydrobromide by HPLC (Fong, Eickhoff, 1989), and in combination with pyridoxine hydrochloride by HPLC (Argekar, Sawant, 2005) have been reported in the literature.…”

“…Some chromatographic methods have been proposed for its determination at trace levels (Argekar, Sawant, 1999;Argekar, Sawant, 2005), mass spectrometry (Hansen et al, 1985), capillary electrophoresis (Tang et al, 2000), UV spectrometry in a micellar medium (Monferrer-Pons et al, 1996) and Fourier transform infrared spectroscopy (FTIR) (Ventura-Gayete et al, 2006) have also been used. Simultaneous determination of DOX in combination with carbinoxamine maleate by spectrophotometry (MonferrerPons at al., 1996), in combination with dextromethorphan hydrobromide by HPTLC (Indrayanto, 1996), in combination with pseudoephedrine hydrochloride and dextromethorphan hydrobromide by HPLC (Fong, Eickhoff, 1989), and in combination with pyridoxine hydrochloride by HPLC (Argekar, Sawant, 2005) have been reported in the literature. Simultaneous determination of DOX in combination with other antihistamines in pharmaceutical preparations by liquid chromatography with a micelar mobile phase of sodium dodecyl sulfate and pentanol (Paciolla et al, 2001;Gil-Augusti et al, 2001A), of DOX in combination with phenethylamine-antihistamine using C8, C18 or cyano columns with a micellar sodium dodecyl sulfate-pentanol mobile phase (Gil-Augusti et al, 2001B), and of DOX in combination with phenylpropanolamine hydrochloride, chlorpheniramine maleate, dextromethorphan hydrobromide, paracetamol and gaifenesin using ion pair reversed-phase by HPLC, have also been reported.…”

P‐Dimethyl Amino Benzaldehyde as a New Chromogenic Reagent for the Determination of Nonsteroidal Anti‐Inflammatory Drug by First‐Order Derivative Spectrophotometry

“…A number of solvents and extractions are involved in the indirect determination of some analgesic-inflammatory drugs by atomic absorption spectrometry (Alpdogan and Sungur 1999). In the HPLC with atomic ionization mass spectrometry (Song et al 2000), HPTLC (Argekar and Sawant 1999), and HPLC with isocratic elution (Mikan et al 2000), three pairs of compounds were either coeluted or unresolved (Song et al 2000), and detection and quantification had to be performed densitometrically (Argekar and Sawant 1999). The relative standard deviation was 1.0% in the nonaqueous titrimetric assay using tetra-n-butylammonium hydroxide as titrant (Cakirer et al 1999).…”

A New Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Pyridoxine Hydrochloride and Meclizine Hydrochloride in Pharmaceutical Solid Dosage Forms

“…The pharmacopeias describe potentiometric, spectrophotometrics and HPLC method for the determination of PYH and MEH individually from the bulk and tablet dosage form. No reversed-phase high-performance liquid chromatography (RP-HPLC) method is reported in pharmacopeias for the simultaneous estimation of PYH and MEH from their combined formulation, though there are various methods for the determination of PYH and MEH by spectrophotometric [7][8][9][10][11][12], voltammetric [13], HPLC [14][15][16], electrophoresis [17], GLC [18], HPTLC [19], and TLC [20] methods in different pharmaceutical dosages forms. The present work describes the development and validation of stability indicating RP-HPLC method, which can quantify PYH and MEH simultaneously in pharmaceutical solid dosage form.…”